Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04055 and BWI76_RS04060 are separated by 262 nucleotides BWI76_RS04060 and BWI76_RS04065 are separated by 20 nucleotides BWI76_RS04065 and BWI76_RS04070 are separated by 31 nucleotides BWI76_RS04070 and BWI76_RS04075 are separated by 28 nucleotides BWI76_RS04075 and BWI76_RS04080 are separated by 167 nucleotides
BWI76_RS04055: BWI76_RS04055 - GGDEF domain-containing protein, at 838,202 to 839,248
_RS04055
BWI76_RS04060: BWI76_RS04060 - hypothetical protein, at 839,511 to 839,750
_RS04060
BWI76_RS04065: BWI76_RS04065 - tRNA-Leu, at 839,771 to 839,857
_RS04065
BWI76_RS04070: BWI76_RS04070 - tRNA-Leu, at 839,889 to 839,975
_RS04070
BWI76_RS04075: BWI76_RS04075 - tRNA-Leu, at 840,004 to 840,090
_RS04075
BWI76_RS04080: BWI76_RS04080 - 16S rRNA (guanine(1207)-N(2))-methyltransferase, at 840,258 to 841,286
_RS04080
Position (kb) 839
840 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 838.534 kb on + strand, within BWI76_RS04055 at 838.535 kb on + strand, within BWI76_RS04055 at 838.605 kb on - strand, within BWI76_RS04055 at 838.605 kb on - strand, within BWI76_RS04055 at 838.607 kb on + strand, within BWI76_RS04055 at 838.607 kb on + strand at 838.610 kb on - strand, within BWI76_RS04055 at 838.615 kb on - strand, within BWI76_RS04055 at 838.616 kb on - strand, within BWI76_RS04055 at 838.624 kb on - strand, within BWI76_RS04055 at 838.660 kb on - strand, within BWI76_RS04055 at 838.662 kb on + strand, within BWI76_RS04055 at 838.677 kb on + strand, within BWI76_RS04055 at 838.690 kb on + strand, within BWI76_RS04055 at 838.690 kb on + strand, within BWI76_RS04055 at 838.698 kb on - strand at 838.705 kb on - strand, within BWI76_RS04055 at 838.706 kb on - strand, within BWI76_RS04055 at 838.732 kb on - strand, within BWI76_RS04055 at 838.759 kb on + strand, within BWI76_RS04055 at 838.767 kb on - strand, within BWI76_RS04055 at 838.769 kb on + strand, within BWI76_RS04055 at 838.783 kb on - strand, within BWI76_RS04055 at 838.802 kb on + strand, within BWI76_RS04055 at 838.803 kb on + strand, within BWI76_RS04055 at 838.810 kb on - strand, within BWI76_RS04055 at 838.811 kb on - strand, within BWI76_RS04055 at 838.811 kb on - strand, within BWI76_RS04055 at 838.820 kb on + strand, within BWI76_RS04055 at 838.820 kb on + strand, within BWI76_RS04055 at 838.820 kb on + strand, within BWI76_RS04055 at 838.820 kb on + strand, within BWI76_RS04055 at 838.828 kb on - strand, within BWI76_RS04055 at 838.848 kb on - strand, within BWI76_RS04055 at 838.848 kb on - strand, within BWI76_RS04055 at 838.848 kb on - strand, within BWI76_RS04055 at 838.850 kb on + strand, within BWI76_RS04055 at 838.850 kb on + strand, within BWI76_RS04055 at 838.850 kb on + strand, within BWI76_RS04055 at 838.850 kb on + strand, within BWI76_RS04055 at 838.857 kb on - strand, within BWI76_RS04055 at 838.867 kb on - strand, within BWI76_RS04055 at 838.867 kb on - strand, within BWI76_RS04055 at 838.871 kb on + strand, within BWI76_RS04055 at 838.871 kb on - strand, within BWI76_RS04055 at 838.876 kb on + strand, within BWI76_RS04055 at 838.876 kb on - strand, within BWI76_RS04055 at 838.942 kb on + strand, within BWI76_RS04055 at 838.987 kb on + strand, within BWI76_RS04055 at 838.996 kb on + strand, within BWI76_RS04055 at 839.001 kb on + strand, within BWI76_RS04055 at 839.001 kb on + strand, within BWI76_RS04055 at 839.001 kb on + strand, within BWI76_RS04055 at 839.004 kb on - strand, within BWI76_RS04055 at 839.004 kb on - strand, within BWI76_RS04055 at 839.009 kb on - strand, within BWI76_RS04055 at 839.009 kb on - strand, within BWI76_RS04055 at 839.009 kb on - strand, within BWI76_RS04055 at 839.011 kb on + strand, within BWI76_RS04055 at 839.011 kb on + strand, within BWI76_RS04055 at 839.058 kb on + strand, within BWI76_RS04055 at 839.058 kb on + strand, within BWI76_RS04055 at 839.058 kb on + strand, within BWI76_RS04055 at 839.058 kb on + strand, within BWI76_RS04055 at 839.058 kb on + strand, within BWI76_RS04055 at 839.058 kb on + strand, within BWI76_RS04055 at 839.066 kb on + strand, within BWI76_RS04055 at 839.066 kb on + strand, within BWI76_RS04055 at 839.066 kb on + strand, within BWI76_RS04055 at 839.066 kb on + strand, within BWI76_RS04055 at 839.066 kb on - strand, within BWI76_RS04055 at 839.074 kb on - strand, within BWI76_RS04055 at 839.075 kb on + strand, within BWI76_RS04055 at 839.075 kb on + strand at 839.075 kb on + strand, within BWI76_RS04055 at 839.075 kb on + strand, within BWI76_RS04055 at 839.075 kb on - strand, within BWI76_RS04055 at 839.080 kb on + strand, within BWI76_RS04055 at 839.083 kb on - strand, within BWI76_RS04055 at 839.083 kb on - strand, within BWI76_RS04055 at 839.083 kb on - strand, within BWI76_RS04055 at 839.083 kb on - strand, within BWI76_RS04055 at 839.088 kb on - strand, within BWI76_RS04055 at 839.088 kb on - strand, within BWI76_RS04055 at 839.098 kb on - strand, within BWI76_RS04055 at 839.101 kb on + strand, within BWI76_RS04055 at 839.106 kb on + strand, within BWI76_RS04055 at 839.109 kb on - strand, within BWI76_RS04055 at 839.111 kb on + strand, within BWI76_RS04055 at 839.111 kb on + strand, within BWI76_RS04055 at 839.111 kb on + strand, within BWI76_RS04055 at 839.114 kb on - strand, within BWI76_RS04055 at 839.114 kb on - strand, within BWI76_RS04055 at 839.114 kb on - strand, within BWI76_RS04055 at 839.114 kb on - strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.116 kb on + strand, within BWI76_RS04055 at 839.119 kb on - strand at 839.119 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.124 kb on - strand, within BWI76_RS04055 at 839.130 kb on + strand, within BWI76_RS04055 at 839.138 kb on - strand, within BWI76_RS04055 at 839.138 kb on - strand, within BWI76_RS04055 at 839.140 kb on - strand, within BWI76_RS04055 at 839.141 kb on - strand, within BWI76_RS04055 at 839.145 kb on - strand at 839.145 kb on - strand at 839.163 kb on + strand at 839.172 kb on + strand at 839.172 kb on + strand at 839.218 kb on + strand at 839.228 kb on - strand at 839.229 kb on + strand at 839.229 kb on + strand at 839.229 kb on + strand at 839.229 kb on + strand at 839.229 kb on + strand at 839.229 kb on + strand at 839.229 kb on + strand at 839.234 kb on + strand at 839.236 kb on - strand at 839.237 kb on - strand at 839.237 kb on - strand at 839.237 kb on - strand at 839.237 kb on - strand at 839.237 kb on - strand at 839.237 kb on - strand at 839.237 kb on - strand at 839.239 kb on + strand at 839.239 kb on + strand at 839.239 kb on + strand at 839.244 kb on + strand at 839.248 kb on + strand at 839.253 kb on + strand at 839.289 kb on - strand at 839.292 kb on + strand at 839.386 kb on + strand at 839.398 kb on + strand at 839.422 kb on + strand at 839.422 kb on + strand at 839.422 kb on + strand at 839.422 kb on + strand at 839.430 kb on - strand at 839.430 kb on - strand at 839.430 kb on - strand at 839.531 kb on - strand at 839.555 kb on - strand, within BWI76_RS04060 at 839.638 kb on - strand, within BWI76_RS04060 at 839.697 kb on + strand, within BWI76_RS04060 at 840.292 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 838,534 + BWI76_RS04055 0.32 +0.6 838,535 + BWI76_RS04055 0.32 +0.4 838,605 - BWI76_RS04055 0.38 +0.3 838,605 - BWI76_RS04055 0.38 +1.2 838,607 + BWI76_RS04055 0.39 -0.6 838,607 + +2.1 838,610 - BWI76_RS04055 0.39 -0.2 838,615 - BWI76_RS04055 0.39 +0.2 838,616 - BWI76_RS04055 0.40 +0.5 838,624 - BWI76_RS04055 0.40 +0.1 838,660 - BWI76_RS04055 0.44 +1.5 838,662 + BWI76_RS04055 0.44 +0.5 838,677 + BWI76_RS04055 0.45 -0.1 838,690 + BWI76_RS04055 0.47 +0.7 838,690 + BWI76_RS04055 0.47 +0.2 838,698 - -0.7 838,705 - BWI76_RS04055 0.48 +1.2 838,706 - BWI76_RS04055 0.48 -0.5 838,732 - BWI76_RS04055 0.51 +0.5 838,759 + BWI76_RS04055 0.53 +0.6 838,767 - BWI76_RS04055 0.54 +0.8 838,769 + BWI76_RS04055 0.54 -0.1 838,783 - BWI76_RS04055 0.55 +1.3 838,802 + BWI76_RS04055 0.57 +0.0 838,803 + BWI76_RS04055 0.57 +0.3 838,810 - BWI76_RS04055 0.58 +0.7 838,811 - BWI76_RS04055 0.58 -0.3 838,811 - BWI76_RS04055 0.58 +0.2 838,820 + BWI76_RS04055 0.59 +0.8 838,820 + BWI76_RS04055 0.59 +0.7 838,820 + BWI76_RS04055 0.59 -0.3 838,820 + BWI76_RS04055 0.59 -0.0 838,828 - BWI76_RS04055 0.60 -0.3 838,848 - BWI76_RS04055 0.62 +0.0 838,848 - BWI76_RS04055 0.62 +0.7 838,848 - BWI76_RS04055 0.62 +0.5 838,850 + BWI76_RS04055 0.62 +0.1 838,850 + BWI76_RS04055 0.62 +0.6 838,850 + BWI76_RS04055 0.62 -1.1 838,850 + BWI76_RS04055 0.62 +0.0 838,857 - BWI76_RS04055 0.63 -0.1 838,867 - BWI76_RS04055 0.64 -0.1 838,867 - BWI76_RS04055 0.64 -0.1 838,871 + BWI76_RS04055 0.64 +0.2 838,871 - BWI76_RS04055 0.64 -0.6 838,876 + BWI76_RS04055 0.64 -0.1 838,876 - BWI76_RS04055 0.64 -0.1 838,942 + BWI76_RS04055 0.71 +0.7 838,987 + BWI76_RS04055 0.75 +0.1 838,996 + BWI76_RS04055 0.76 +1.1 839,001 + BWI76_RS04055 0.76 -0.4 839,001 + BWI76_RS04055 0.76 -0.3 839,001 + BWI76_RS04055 0.76 -2.8 839,004 - BWI76_RS04055 0.77 +0.4 839,004 - BWI76_RS04055 0.77 +2.5 839,009 - BWI76_RS04055 0.77 -0.2 839,009 - BWI76_RS04055 0.77 +0.3 839,009 - BWI76_RS04055 0.77 -0.8 839,011 + BWI76_RS04055 0.77 -0.3 839,011 + BWI76_RS04055 0.77 +0.3 839,058 + BWI76_RS04055 0.82 +0.7 839,058 + BWI76_RS04055 0.82 +0.0 839,058 + BWI76_RS04055 0.82 -0.9 839,058 + BWI76_RS04055 0.82 +0.1 839,058 + BWI76_RS04055 0.82 +0.3 839,058 + BWI76_RS04055 0.82 +0.9 839,066 + BWI76_RS04055 0.83 -0.6 839,066 + BWI76_RS04055 0.83 -0.9 839,066 + BWI76_RS04055 0.83 +0.2 839,066 + BWI76_RS04055 0.83 -0.1 839,066 - BWI76_RS04055 0.83 +0.4 839,074 - BWI76_RS04055 0.83 +0.4 839,075 + BWI76_RS04055 0.83 +1.6 839,075 + +1.9 839,075 + BWI76_RS04055 0.83 +0.4 839,075 + BWI76_RS04055 0.83 +0.4 839,075 - BWI76_RS04055 0.83 +0.1 839,080 + BWI76_RS04055 0.84 -0.0 839,083 - BWI76_RS04055 0.84 +0.5 839,083 - BWI76_RS04055 0.84 +0.3 839,083 - BWI76_RS04055 0.84 +0.5 839,083 - BWI76_RS04055 0.84 +0.9 839,088 - BWI76_RS04055 0.85 +0.7 839,088 - BWI76_RS04055 0.85 +1.5 839,098 - BWI76_RS04055 0.86 +0.0 839,101 + BWI76_RS04055 0.86 -1.1 839,106 + BWI76_RS04055 0.86 -0.1 839,109 - BWI76_RS04055 0.87 +0.1 839,111 + BWI76_RS04055 0.87 +0.3 839,111 + BWI76_RS04055 0.87 +0.5 839,111 + BWI76_RS04055 0.87 +0.6 839,114 - BWI76_RS04055 0.87 +0.2 839,114 - BWI76_RS04055 0.87 +0.3 839,114 - BWI76_RS04055 0.87 +0.1 839,114 - BWI76_RS04055 0.87 +1.1 839,116 + BWI76_RS04055 0.87 -0.2 839,116 + BWI76_RS04055 0.87 -0.6 839,116 + BWI76_RS04055 0.87 +0.1 839,116 + BWI76_RS04055 0.87 -0.1 839,116 + BWI76_RS04055 0.87 +0.2 839,116 + BWI76_RS04055 0.87 +0.6 839,116 + BWI76_RS04055 0.87 +1.4 839,116 + BWI76_RS04055 0.87 +0.3 839,116 + BWI76_RS04055 0.87 +0.7 839,116 + BWI76_RS04055 0.87 -0.3 839,116 + BWI76_RS04055 0.87 +0.3 839,119 - +1.1 839,119 - BWI76_RS04055 0.88 +0.3 839,124 - BWI76_RS04055 0.88 +0.5 839,124 - BWI76_RS04055 0.88 +0.6 839,124 - BWI76_RS04055 0.88 +1.1 839,124 - BWI76_RS04055 0.88 +0.4 839,124 - BWI76_RS04055 0.88 +0.8 839,124 - BWI76_RS04055 0.88 +0.1 839,124 - BWI76_RS04055 0.88 +0.5 839,124 - BWI76_RS04055 0.88 +0.1 839,124 - BWI76_RS04055 0.88 +0.6 839,124 - BWI76_RS04055 0.88 +0.1 839,124 - BWI76_RS04055 0.88 +0.4 839,124 - BWI76_RS04055 0.88 -1.6 839,124 - BWI76_RS04055 0.88 -0.1 839,124 - BWI76_RS04055 0.88 -0.5 839,124 - BWI76_RS04055 0.88 +0.6 839,130 + BWI76_RS04055 0.89 +1.0 839,138 - BWI76_RS04055 0.89 -0.0 839,138 - BWI76_RS04055 0.89 +0.0 839,140 - BWI76_RS04055 0.90 +0.3 839,141 - BWI76_RS04055 0.90 +1.0 839,145 - -0.2 839,145 - +1.2 839,163 + +1.2 839,172 + +0.4 839,172 + +1.2 839,218 + -0.1 839,228 - -0.3 839,229 + -0.2 839,229 + +0.1 839,229 + +0.7 839,229 + -0.3 839,229 + +0.7 839,229 + +0.4 839,229 + -0.5 839,234 + -0.2 839,236 - +0.0 839,237 - +0.6 839,237 - +0.2 839,237 - -1.5 839,237 - -0.6 839,237 - -0.1 839,237 - -0.2 839,237 - -0.1 839,239 + +0.1 839,239 + +1.3 839,239 + -0.3 839,244 + +0.3 839,248 + +0.5 839,253 + -0.0 839,289 - +0.4 839,292 + +1.4 839,386 + -0.0 839,398 + -0.7 839,422 + +0.9 839,422 + +0.9 839,422 + +0.8 839,422 + +0.3 839,430 - -0.6 839,430 - +0.3 839,430 - -0.5 839,531 - -0.7 839,555 - BWI76_RS04060 0.18 -1.3 839,638 - BWI76_RS04060 0.53 -0.1 839,697 + BWI76_RS04060 0.78 -1.1 840,292 - -0.2
Or see this region's nucleotide sequence