Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04015 and BWI76_RS04020 are separated by 284 nucleotides BWI76_RS04020 and BWI76_RS04025 are separated by 23 nucleotides
BWI76_RS04015: BWI76_RS04015 - membrane protein, at 830,331 to 831,107
_RS04015
BWI76_RS04020: BWI76_RS04020 - 4-hydroxybenzoate 3-monooxygenase, at 831,392 to 832,576
_RS04020
BWI76_RS04025: BWI76_RS04025 - AraC family transcriptional regulator, at 832,600 to 833,458
_RS04025
Position (kb) 831
832
833 Strain fitness (log2 ratio) -2
-1
0
1 at 830.566 kb on - strand, within BWI76_RS04015 at 830.566 kb on - strand, within BWI76_RS04015 at 830.625 kb on + strand, within BWI76_RS04015 at 830.763 kb on + strand, within BWI76_RS04015 at 830.778 kb on - strand, within BWI76_RS04015 at 830.926 kb on - strand, within BWI76_RS04015 at 830.934 kb on - strand, within BWI76_RS04015 at 830.950 kb on - strand, within BWI76_RS04015 at 830.973 kb on - strand, within BWI76_RS04015 at 831.008 kb on + strand, within BWI76_RS04015 at 831.063 kb on - strand at 831.117 kb on - strand at 831.197 kb on - strand at 831.382 kb on + strand at 831.474 kb on + strand at 831.488 kb on - strand at 831.509 kb on - strand at 831.509 kb on - strand at 831.546 kb on + strand, within BWI76_RS04020 at 831.625 kb on + strand, within BWI76_RS04020 at 831.659 kb on + strand, within BWI76_RS04020 at 831.996 kb on - strand, within BWI76_RS04020 at 832.095 kb on - strand, within BWI76_RS04020 at 832.180 kb on - strand, within BWI76_RS04020 at 832.190 kb on - strand, within BWI76_RS04020 at 832.241 kb on - strand, within BWI76_RS04020 at 832.285 kb on + strand, within BWI76_RS04020 at 832.422 kb on + strand, within BWI76_RS04020 at 832.431 kb on - strand, within BWI76_RS04020 at 832.514 kb on + strand at 832.528 kb on - strand at 832.528 kb on - strand at 832.528 kb on - strand at 832.528 kb on - strand at 832.528 kb on - strand at 832.571 kb on + strand at 832.571 kb on + strand at 832.579 kb on - strand at 832.579 kb on - strand at 832.687 kb on + strand, within BWI76_RS04025 at 832.786 kb on + strand, within BWI76_RS04025 at 832.827 kb on - strand, within BWI76_RS04025 at 832.876 kb on + strand, within BWI76_RS04025 at 832.885 kb on - strand, within BWI76_RS04025 at 832.897 kb on - strand, within BWI76_RS04025 at 832.908 kb on - strand, within BWI76_RS04025 at 832.959 kb on - strand, within BWI76_RS04025 at 832.999 kb on + strand, within BWI76_RS04025 at 832.999 kb on + strand, within BWI76_RS04025 at 833.007 kb on - strand, within BWI76_RS04025 at 833.007 kb on - strand, within BWI76_RS04025 at 833.025 kb on - strand, within BWI76_RS04025 at 833.055 kb on + strand, within BWI76_RS04025 at 833.064 kb on - strand, within BWI76_RS04025 at 833.065 kb on + strand, within BWI76_RS04025 at 833.065 kb on + strand, within BWI76_RS04025 at 833.073 kb on - strand, within BWI76_RS04025 at 833.073 kb on - strand, within BWI76_RS04025 at 833.073 kb on - strand at 833.075 kb on + strand, within BWI76_RS04025 at 833.110 kb on + strand, within BWI76_RS04025 at 833.117 kb on + strand, within BWI76_RS04025 at 833.122 kb on + strand, within BWI76_RS04025 at 833.122 kb on + strand, within BWI76_RS04025 at 833.140 kb on + strand, within BWI76_RS04025 at 833.143 kb on - strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.192 kb on + strand, within BWI76_RS04025 at 833.200 kb on - strand, within BWI76_RS04025 at 833.200 kb on - strand, within BWI76_RS04025 at 833.200 kb on - strand, within BWI76_RS04025 at 833.200 kb on - strand, within BWI76_RS04025 at 833.200 kb on - strand, within BWI76_RS04025 at 833.200 kb on - strand, within BWI76_RS04025 at 833.200 kb on - strand, within BWI76_RS04025 at 833.200 kb on - strand, within BWI76_RS04025 at 833.214 kb on + strand, within BWI76_RS04025 at 833.215 kb on - strand, within BWI76_RS04025 at 833.215 kb on - strand, within BWI76_RS04025 at 833.220 kb on + strand, within BWI76_RS04025 at 833.239 kb on - strand, within BWI76_RS04025 at 833.274 kb on + strand, within BWI76_RS04025 at 833.274 kb on + strand, within BWI76_RS04025 at 833.274 kb on + strand, within BWI76_RS04025 at 833.274 kb on + strand, within BWI76_RS04025 at 833.274 kb on + strand, within BWI76_RS04025 at 833.279 kb on + strand, within BWI76_RS04025 at 833.282 kb on - strand, within BWI76_RS04025 at 833.287 kb on - strand, within BWI76_RS04025 at 833.292 kb on - strand, within BWI76_RS04025 at 833.301 kb on + strand, within BWI76_RS04025 at 833.302 kb on - strand, within BWI76_RS04025 at 833.309 kb on - strand, within BWI76_RS04025 at 833.324 kb on + strand, within BWI76_RS04025 at 833.325 kb on + strand, within BWI76_RS04025 at 833.325 kb on + strand, within BWI76_RS04025 at 833.329 kb on + strand, within BWI76_RS04025 at 833.330 kb on + strand, within BWI76_RS04025 at 833.330 kb on + strand, within BWI76_RS04025 at 833.332 kb on + strand, within BWI76_RS04025 at 833.332 kb on + strand, within BWI76_RS04025 at 833.332 kb on + strand, within BWI76_RS04025 at 833.333 kb on - strand, within BWI76_RS04025 at 833.335 kb on - strand, within BWI76_RS04025 at 833.338 kb on - strand, within BWI76_RS04025 at 833.340 kb on - strand, within BWI76_RS04025 at 833.340 kb on - strand, within BWI76_RS04025 at 833.340 kb on - strand, within BWI76_RS04025 at 833.340 kb on - strand, within BWI76_RS04025 at 833.374 kb on + strand at 833.376 kb on - strand at 833.415 kb on + strand at 833.421 kb on - strand at 833.421 kb on - strand at 833.421 kb on - strand at 833.487 kb on - strand at 833.535 kb on + strand at 833.541 kb on + strand at 833.541 kb on + strand at 833.549 kb on - strand at 833.565 kb on + strand at 833.570 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 830,566 - BWI76_RS04015 0.30 +0.2 830,566 - BWI76_RS04015 0.30 +0.2 830,625 + BWI76_RS04015 0.38 +0.3 830,763 + BWI76_RS04015 0.56 +0.7 830,778 - BWI76_RS04015 0.58 +0.0 830,926 - BWI76_RS04015 0.77 +0.5 830,934 - BWI76_RS04015 0.78 +0.1 830,950 - BWI76_RS04015 0.80 -1.2 830,973 - BWI76_RS04015 0.83 +0.1 831,008 + BWI76_RS04015 0.87 +0.2 831,063 - -0.3 831,117 - +0.1 831,197 - -0.1 831,382 + -0.3 831,474 + +0.0 831,488 - +0.4 831,509 - +1.7 831,509 - +0.7 831,546 + BWI76_RS04020 0.13 +0.5 831,625 + BWI76_RS04020 0.20 +0.8 831,659 + BWI76_RS04020 0.23 +0.0 831,996 - BWI76_RS04020 0.51 -1.7 832,095 - BWI76_RS04020 0.59 +0.4 832,180 - BWI76_RS04020 0.66 +0.4 832,190 - BWI76_RS04020 0.67 -0.2 832,241 - BWI76_RS04020 0.72 -0.2 832,285 + BWI76_RS04020 0.75 -0.2 832,422 + BWI76_RS04020 0.87 +0.1 832,431 - BWI76_RS04020 0.88 +0.6 832,514 + +0.4 832,528 - +0.1 832,528 - -0.1 832,528 - -0.0 832,528 - -0.4 832,528 - +0.1 832,571 + +0.1 832,571 + -0.1 832,579 - -1.3 832,579 - +0.0 832,687 + BWI76_RS04025 0.10 +0.2 832,786 + BWI76_RS04025 0.22 +0.5 832,827 - BWI76_RS04025 0.26 +0.5 832,876 + BWI76_RS04025 0.32 -1.2 832,885 - BWI76_RS04025 0.33 +0.4 832,897 - BWI76_RS04025 0.35 -0.7 832,908 - BWI76_RS04025 0.36 -0.1 832,959 - BWI76_RS04025 0.42 -0.6 832,999 + BWI76_RS04025 0.46 -0.6 832,999 + BWI76_RS04025 0.46 +0.9 833,007 - BWI76_RS04025 0.47 -0.4 833,007 - BWI76_RS04025 0.47 +0.2 833,025 - BWI76_RS04025 0.49 +0.1 833,055 + BWI76_RS04025 0.53 +0.2 833,064 - BWI76_RS04025 0.54 +1.0 833,065 + BWI76_RS04025 0.54 +0.5 833,065 + BWI76_RS04025 0.54 -0.4 833,073 - BWI76_RS04025 0.55 -0.0 833,073 - BWI76_RS04025 0.55 -0.4 833,073 - -1.4 833,075 + BWI76_RS04025 0.55 +1.6 833,110 + BWI76_RS04025 0.59 +1.1 833,117 + BWI76_RS04025 0.60 -1.5 833,122 + BWI76_RS04025 0.61 +0.2 833,122 + BWI76_RS04025 0.61 +0.5 833,140 + BWI76_RS04025 0.63 -0.7 833,143 - BWI76_RS04025 0.63 +0.1 833,192 + BWI76_RS04025 0.69 -0.0 833,192 + BWI76_RS04025 0.69 -0.5 833,192 + BWI76_RS04025 0.69 +0.4 833,192 + BWI76_RS04025 0.69 +0.2 833,192 + BWI76_RS04025 0.69 +0.4 833,192 + BWI76_RS04025 0.69 -0.3 833,192 + BWI76_RS04025 0.69 +0.1 833,192 + BWI76_RS04025 0.69 -0.4 833,192 + BWI76_RS04025 0.69 -0.5 833,200 - BWI76_RS04025 0.70 +0.1 833,200 - BWI76_RS04025 0.70 -0.9 833,200 - BWI76_RS04025 0.70 +0.1 833,200 - BWI76_RS04025 0.70 -0.2 833,200 - BWI76_RS04025 0.70 +0.7 833,200 - BWI76_RS04025 0.70 +0.1 833,200 - BWI76_RS04025 0.70 -0.2 833,200 - BWI76_RS04025 0.70 +0.1 833,214 + BWI76_RS04025 0.71 +0.1 833,215 - BWI76_RS04025 0.72 +0.3 833,215 - BWI76_RS04025 0.72 -0.2 833,220 + BWI76_RS04025 0.72 -0.4 833,239 - BWI76_RS04025 0.74 +0.1 833,274 + BWI76_RS04025 0.78 +0.4 833,274 + BWI76_RS04025 0.78 +0.2 833,274 + BWI76_RS04025 0.78 +0.4 833,274 + BWI76_RS04025 0.78 -0.1 833,274 + BWI76_RS04025 0.78 -1.9 833,279 + BWI76_RS04025 0.79 -0.5 833,282 - BWI76_RS04025 0.79 +0.6 833,287 - BWI76_RS04025 0.80 -1.2 833,292 - BWI76_RS04025 0.81 +0.2 833,301 + BWI76_RS04025 0.82 -0.5 833,302 - BWI76_RS04025 0.82 +0.2 833,309 - BWI76_RS04025 0.83 +0.6 833,324 + BWI76_RS04025 0.84 +1.5 833,325 + BWI76_RS04025 0.84 +0.4 833,325 + BWI76_RS04025 0.84 -1.2 833,329 + BWI76_RS04025 0.85 -0.1 833,330 + BWI76_RS04025 0.85 -0.0 833,330 + BWI76_RS04025 0.85 -0.3 833,332 + BWI76_RS04025 0.85 +0.6 833,332 + BWI76_RS04025 0.85 -0.8 833,332 + BWI76_RS04025 0.85 -0.6 833,333 - BWI76_RS04025 0.85 -0.3 833,335 - BWI76_RS04025 0.86 -0.0 833,338 - BWI76_RS04025 0.86 -0.8 833,340 - BWI76_RS04025 0.86 -0.1 833,340 - BWI76_RS04025 0.86 +0.0 833,340 - BWI76_RS04025 0.86 -0.5 833,340 - BWI76_RS04025 0.86 +0.6 833,374 + +0.6 833,376 - +0.6 833,415 + +0.7 833,421 - +0.2 833,421 - +0.9 833,421 - -1.0 833,487 - +0.3 833,535 + +0.7 833,541 + -1.3 833,541 + +0.3 833,549 - +1.1 833,565 + -0.7 833,570 + -0.2
Or see this region's nucleotide sequence