Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00735 and BWI76_RS00740 are separated by 60 nucleotides BWI76_RS00740 and BWI76_RS00745 are separated by 92 nucleotides BWI76_RS00745 and BWI76_RS00750 are separated by 244 nucleotides
BWI76_RS00735: BWI76_RS00735 - ATP-dependent 6-phosphofructokinase, at 149,499 to 150,461
_RS00735
BWI76_RS00740: BWI76_RS00740 - ribonuclease E activity regulator RraA, at 150,522 to 151,007
_RS00740
BWI76_RS00745: BWI76_RS00745 - 1,4-dihydroxy-2-naphthoate polyprenyltransferase, at 151,100 to 152,026
_RS00745
BWI76_RS00750: BWI76_RS00750 - ATP-dependent protease ATP-binding subunit HslU, at 152,271 to 153,605
_RS00750
Position (kb) 151
152
153 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 150.559 kb on - strand at 150.683 kb on - strand, within BWI76_RS00740 at 150.827 kb on + strand, within BWI76_RS00740 at 150.827 kb on - strand, within BWI76_RS00740 at 150.867 kb on - strand, within BWI76_RS00740 at 150.867 kb on - strand, within BWI76_RS00740 at 150.963 kb on - strand at 151.169 kb on - strand at 151.176 kb on - strand at 151.316 kb on - strand, within BWI76_RS00745 at 151.316 kb on - strand, within BWI76_RS00745 at 151.443 kb on + strand, within BWI76_RS00745 at 151.489 kb on + strand, within BWI76_RS00745 at 151.489 kb on + strand, within BWI76_RS00745 at 151.497 kb on - strand, within BWI76_RS00745 at 151.497 kb on - strand, within BWI76_RS00745 at 152.095 kb on + strand at 152.172 kb on + strand at 152.206 kb on - strand at 152.239 kb on + strand at 152.239 kb on + strand at 152.243 kb on - strand at 152.247 kb on - strand at 152.481 kb on + strand, within BWI76_RS00750 at 152.533 kb on + strand, within BWI76_RS00750 at 152.745 kb on - strand, within BWI76_RS00750 at 152.784 kb on - strand, within BWI76_RS00750 at 152.931 kb on - strand, within BWI76_RS00750
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 150,559 - +1.0 150,683 - BWI76_RS00740 0.33 +0.0 150,827 + BWI76_RS00740 0.63 -0.0 150,827 - BWI76_RS00740 0.63 +0.0 150,867 - BWI76_RS00740 0.71 +0.7 150,867 - BWI76_RS00740 0.71 -0.4 150,963 - +0.8 151,169 - -0.9 151,176 - -1.5 151,316 - BWI76_RS00745 0.23 -3.6 151,316 - BWI76_RS00745 0.23 -1.5 151,443 + BWI76_RS00745 0.37 -0.9 151,489 + BWI76_RS00745 0.42 -0.4 151,489 + BWI76_RS00745 0.42 -0.9 151,497 - BWI76_RS00745 0.43 -0.9 151,497 - BWI76_RS00745 0.43 -1.7 152,095 + -0.0 152,172 + -0.8 152,206 - -0.1 152,239 + +0.3 152,239 + -0.2 152,243 - +0.6 152,247 - -0.1 152,481 + BWI76_RS00750 0.16 -0.3 152,533 + BWI76_RS00750 0.20 +0.4 152,745 - BWI76_RS00750 0.36 -0.3 152,784 - BWI76_RS00750 0.38 -0.4 152,931 - BWI76_RS00750 0.49 -0.5
Or see this region's nucleotide sequence