Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4703

Experiment: BHIS with Lincomycin 0.0015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4701 and BT4702 are separated by 3 nucleotidesBT4702 and BT4703 are separated by 3 nucleotidesBT4703 and BT4704 overlap by 4 nucleotides BT4701: BT4701 - 2-oxoglutarate decarboxylase and 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (NCBI ptt file), at 6,170,350 to 6,172,017 BT4701 BT4702: BT4702 - dihydroxynapthoic acid synthetase (NCBI ptt file), at 6,172,021 to 6,172,845 BT4702 BT4703: BT4703 - chloromuconate cycloisomerase (NCBI ptt file), at 6,172,849 to 6,173,874 BT4703 BT4704: BT4704 - O-succinylbenzoic acid--CoA ligase (NCBI ptt file), at 6,173,871 to 6,174,977 BT4704 Position (kb) 6172 6173 6174Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 6172.020 kb on + strandat 6172.078 kb on + strandat 6172.319 kb on + strand, within BT4702at 6172.535 kb on + strand, within BT4702at 6172.593 kb on + strand, within BT4702at 6172.599 kb on - strand, within BT4702at 6172.756 kb on + strand, within BT4702at 6172.760 kb on - strand, within BT4702at 6172.809 kb on + strandat 6173.053 kb on - strand, within BT4703at 6173.184 kb on + strand, within BT4703at 6173.211 kb on + strand, within BT4703at 6173.385 kb on - strand, within BT4703at 6173.465 kb on + strand, within BT4703at 6173.521 kb on - strand, within BT4703at 6173.565 kb on - strand, within BT4703at 6173.570 kb on - strand, within BT4703at 6173.571 kb on + strand, within BT4703at 6173.574 kb on + strand, within BT4703at 6173.575 kb on - strand, within BT4703at 6173.626 kb on - strand, within BT4703at 6173.628 kb on + strand, within BT4703at 6173.711 kb on + strand, within BT4703at 6173.807 kb on + strandat 6173.820 kb on + strandat 6173.825 kb on + strandat 6173.922 kb on + strandat 6174.068 kb on + strand, within BT4704at 6174.158 kb on - strand, within BT4704at 6174.178 kb on - strand, within BT4704at 6174.233 kb on - strand, within BT4704at 6174.235 kb on + strand, within BT4704at 6174.236 kb on - strand, within BT4704at 6174.313 kb on - strand, within BT4704at 6174.313 kb on - strand, within BT4704at 6174.317 kb on + strand, within BT4704at 6174.318 kb on - strand, within BT4704at 6174.318 kb on - strand, within BT4704at 6174.371 kb on + strand, within BT4704at 6174.513 kb on + strand, within BT4704at 6174.517 kb on - strand, within BT4704at 6174.569 kb on + strand, within BT4704at 6174.570 kb on - strand, within BT4704at 6174.612 kb on - strand, within BT4704at 6174.674 kb on + strand, within BT4704at 6174.675 kb on - strand, within BT4704at 6174.687 kb on - strand, within BT4704at 6174.815 kb on - strand, within BT4704at 6174.831 kb on - strand, within BT4704at 6174.855 kb on + strand, within BT4704at 6174.856 kb on - strand, within BT4704at 6174.859 kb on - strand, within BT4704at 6174.873 kb on - strandat 6174.873 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Lincomycin 0.0015625 mM
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6,172,020 + -1.8
6,172,078 + -1.3
6,172,319 + BT4702 0.36 -1.2
6,172,535 + BT4702 0.62 -1.8
6,172,593 + BT4702 0.69 +0.2
6,172,599 - BT4702 0.70 -0.8
6,172,756 + BT4702 0.89 -1.3
6,172,760 - BT4702 0.90 -0.8
6,172,809 + -1.9
6,173,053 - BT4703 0.20 +0.2
6,173,184 + BT4703 0.33 -3.5
6,173,211 + BT4703 0.35 -2.9
6,173,385 - BT4703 0.52 -2.2
6,173,465 + BT4703 0.60 -2.3
6,173,521 - BT4703 0.65 -2.0
6,173,565 - BT4703 0.70 +0.2
6,173,570 - BT4703 0.70 -1.8
6,173,571 + BT4703 0.70 -1.3
6,173,574 + BT4703 0.71 -0.8
6,173,575 - BT4703 0.71 -1.8
6,173,626 - BT4703 0.76 -1.3
6,173,628 + BT4703 0.76 +2.2
6,173,711 + BT4703 0.84 -1.3
6,173,807 + -0.8
6,173,820 + +0.2
6,173,825 + -1.3
6,173,922 + -1.8
6,174,068 + BT4704 0.18 -2.1
6,174,158 - BT4704 0.26 -2.2
6,174,178 - BT4704 0.28 -0.3
6,174,233 - BT4704 0.33 +0.2
6,174,235 + BT4704 0.33 +0.2
6,174,236 - BT4704 0.33 -1.3
6,174,313 - BT4704 0.40 -1.9
6,174,313 - BT4704 0.40 -1.3
6,174,317 + BT4704 0.40 -1.8
6,174,318 - BT4704 0.40 -1.3
6,174,318 - BT4704 0.40 -0.8
6,174,371 + BT4704 0.45 -0.6
6,174,513 + BT4704 0.58 -1.2
6,174,517 - BT4704 0.58 -2.3
6,174,569 + BT4704 0.63 -1.3
6,174,570 - BT4704 0.63 -3.5
6,174,612 - BT4704 0.67 -0.8
6,174,674 + BT4704 0.73 -1.3
6,174,675 - BT4704 0.73 +0.4
6,174,687 - BT4704 0.74 +0.2
6,174,815 - BT4704 0.85 -0.8
6,174,831 - BT4704 0.87 +0.2
6,174,855 + BT4704 0.89 -2.5
6,174,856 - BT4704 0.89 -1.8
6,174,859 - BT4704 0.89 -2.3
6,174,873 - -1.3
6,174,873 - +1.2

Or see this region's nucleotide sequence