Experiment: MoLS4 with L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrA and DVU1988 are separated by 107 nucleotides DVU1988 and DVU1990 are separated by 176 nucleotides DVU1990 and tRNA-Leu are separated by 65 nucleotides
DVU1987: uvrA - excinuclease ABC, A subunit (TIGR), at 2,065,139 to 2,068,009
uvrA
DVU1988: DVU1988 - membrane protein, putative (TIGR), at 2,068,117 to 2,068,803
DVU1988
DVU1990: DVU1990 - hypothetical protein (TIGR), at 2,068,980 to 2,069,627
DVU1990
DVU_tRNA-Leu-5: tRNA-Leu - tRNA with anticodon CAA for Leu (VIMSS-AUTO), at 2,069,693 to 2,069,779
tRNA-Leu
Position (kb)
2068
2069 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2067.142 kb on - strand, within uvrA at 2067.166 kb on + strand, within uvrA at 2067.166 kb on + strand, within uvrA at 2067.166 kb on + strand, within uvrA at 2067.169 kb on - strand, within uvrA at 2067.174 kb on - strand, within uvrA at 2067.174 kb on - strand, within uvrA at 2067.174 kb on - strand, within uvrA at 2067.174 kb on - strand, within uvrA at 2067.198 kb on + strand, within uvrA at 2067.200 kb on + strand, within uvrA at 2067.321 kb on + strand, within uvrA at 2067.329 kb on - strand, within uvrA at 2067.500 kb on - strand, within uvrA at 2067.518 kb on + strand, within uvrA at 2067.525 kb on + strand, within uvrA at 2067.525 kb on + strand, within uvrA at 2067.527 kb on + strand, within uvrA at 2067.527 kb on + strand, within uvrA at 2067.570 kb on + strand, within uvrA at 2067.738 kb on - strand at 2067.904 kb on + strand at 2067.957 kb on + strand at 2067.973 kb on + strand at 2068.100 kb on - strand at 2068.132 kb on + strand at 2068.140 kb on - strand at 2068.145 kb on + strand at 2068.145 kb on + strand at 2068.158 kb on - strand at 2068.257 kb on + strand, within DVU1988 at 2068.268 kb on + strand, within DVU1988 at 2068.268 kb on + strand, within DVU1988 at 2068.366 kb on - strand, within DVU1988 at 2068.391 kb on + strand, within DVU1988 at 2068.407 kb on + strand, within DVU1988 at 2068.474 kb on + strand, within DVU1988 at 2068.482 kb on - strand, within DVU1988 at 2068.487 kb on + strand, within DVU1988 at 2068.487 kb on + strand, within DVU1988 at 2068.735 kb on + strand at 2068.780 kb on - strand at 2068.892 kb on + strand at 2068.892 kb on + strand at 2068.900 kb on - strand at 2068.962 kb on + strand at 2068.970 kb on - strand at 2068.978 kb on - strand at 2068.997 kb on + strand at 2069.050 kb on + strand, within DVU1990 at 2069.159 kb on - strand, within DVU1990 at 2069.200 kb on + strand, within DVU1990 at 2069.208 kb on - strand, within DVU1990 at 2069.270 kb on + strand, within DVU1990 at 2069.289 kb on + strand, within DVU1990 at 2069.291 kb on + strand, within DVU1990 at 2069.315 kb on - strand, within DVU1990 at 2069.400 kb on - strand, within DVU1990 at 2069.402 kb on + strand, within DVU1990 at 2069.422 kb on + strand, within DVU1990 at 2069.422 kb on + strand, within DVU1990 at 2069.441 kb on + strand, within DVU1990 at 2069.488 kb on - strand, within DVU1990 at 2069.490 kb on + strand, within DVU1990 at 2069.515 kb on - strand, within DVU1990 at 2069.519 kb on + strand, within DVU1990 at 2069.594 kb on + strand at 2069.614 kb on - strand at 2069.624 kb on - strand at 2069.633 kb on + strand at 2069.646 kb on - strand at 2069.669 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Tryptophan (N) remove 2,067,142 - uvrA DVU1987 0.70 +0.9 2,067,166 + uvrA DVU1987 0.71 +0.3 2,067,166 + uvrA DVU1987 0.71 -0.4 2,067,166 + uvrA DVU1987 0.71 +0.5 2,067,169 - uvrA DVU1987 0.71 +0.3 2,067,174 - uvrA DVU1987 0.71 -0.7 2,067,174 - uvrA DVU1987 0.71 -0.3 2,067,174 - uvrA DVU1987 0.71 -0.1 2,067,174 - uvrA DVU1987 0.71 -0.3 2,067,198 + uvrA DVU1987 0.72 -0.5 2,067,200 + uvrA DVU1987 0.72 +0.6 2,067,321 + uvrA DVU1987 0.76 +0.4 2,067,329 - uvrA DVU1987 0.76 -0.7 2,067,500 - uvrA DVU1987 0.82 -0.2 2,067,518 + uvrA DVU1987 0.83 +0.1 2,067,525 + uvrA DVU1987 0.83 -0.7 2,067,525 + uvrA DVU1987 0.83 -0.2 2,067,527 + uvrA DVU1987 0.83 -0.3 2,067,527 + uvrA DVU1987 0.83 +0.5 2,067,570 + uvrA DVU1987 0.85 -0.1 2,067,738 - +0.7 2,067,904 + -0.3 2,067,957 + +2.5 2,067,973 + +1.1 2,068,100 - +0.4 2,068,132 + -0.4 2,068,140 - +0.1 2,068,145 + -0.0 2,068,145 + -0.3 2,068,158 - -0.4 2,068,257 + DVU1988 0.20 -0.5 2,068,268 + DVU1988 0.22 -0.4 2,068,268 + DVU1988 0.22 -0.2 2,068,366 - DVU1988 0.36 +1.6 2,068,391 + DVU1988 0.40 -0.8 2,068,407 + DVU1988 0.42 -0.6 2,068,474 + DVU1988 0.52 -0.8 2,068,482 - DVU1988 0.53 -0.3 2,068,487 + DVU1988 0.54 -0.4 2,068,487 + DVU1988 0.54 +1.0 2,068,735 + -0.8 2,068,780 - +1.6 2,068,892 + +0.1 2,068,892 + +0.0 2,068,900 - -0.0 2,068,962 + +1.1 2,068,970 - -0.3 2,068,978 - -0.2 2,068,997 + +0.8 2,069,050 + DVU1990 0.11 +2.9 2,069,159 - DVU1990 0.28 +0.1 2,069,200 + DVU1990 0.34 +0.1 2,069,208 - DVU1990 0.35 +0.2 2,069,270 + DVU1990 0.45 -0.8 2,069,289 + DVU1990 0.48 -0.9 2,069,291 + DVU1990 0.48 -0.1 2,069,315 - DVU1990 0.52 +1.2 2,069,400 - DVU1990 0.65 +1.0 2,069,402 + DVU1990 0.65 -1.2 2,069,422 + DVU1990 0.68 -0.4 2,069,422 + DVU1990 0.68 -0.7 2,069,441 + DVU1990 0.71 -1.3 2,069,488 - DVU1990 0.78 -0.4 2,069,490 + DVU1990 0.79 +0.5 2,069,515 - DVU1990 0.83 +0.4 2,069,519 + DVU1990 0.83 -0.1 2,069,594 + +0.5 2,069,614 - +0.5 2,069,624 - -0.4 2,069,633 + +1.2 2,069,646 - -1.1 2,069,669 + -0.7
Or see this region's nucleotide sequence