Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07470

Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07460 and BWI76_RS07465 overlap by 1 nucleotidesBWI76_RS07465 and BWI76_RS07470 are separated by 190 nucleotidesBWI76_RS07470 and BWI76_RS07475 are separated by 32 nucleotidesBWI76_RS07475 and BWI76_RS07480 are separated by 36 nucleotides BWI76_RS07460: BWI76_RS07460 - hypothetical protein, at 1,574,562 to 1,574,930 _RS07460 BWI76_RS07465: BWI76_RS07465 - TetR family transcriptional regulator, at 1,574,930 to 1,575,511 _RS07465 BWI76_RS07470: BWI76_RS07470 - MBL fold metallo-hydrolase, at 1,575,702 to 1,576,802 _RS07470 BWI76_RS07475: BWI76_RS07475 - DNA-binding transcriptional regulator RamA, at 1,576,835 to 1,577,176 _RS07475 BWI76_RS07480: BWI76_RS07480 - TonB-dependent siderophore receptor, at 1,577,213 to 1,579,357 _RS07480 Position (kb) 1575 1576 1577Strain fitness (log2 ratio) -2 -1 0 1at 1574.702 kb on + strand, within BWI76_RS07460at 1574.900 kb on + strandat 1574.947 kb on - strandat 1575.250 kb on - strand, within BWI76_RS07465at 1575.306 kb on + strand, within BWI76_RS07465at 1575.514 kb on - strandat 1575.575 kb on - strandat 1575.835 kb on - strand, within BWI76_RS07470at 1575.968 kb on + strand, within BWI76_RS07470at 1576.364 kb on + strand, within BWI76_RS07470at 1576.519 kb on - strand, within BWI76_RS07470at 1576.693 kb on + strandat 1576.703 kb on + strandat 1576.801 kb on + strandat 1576.969 kb on + strand, within BWI76_RS07475at 1577.368 kb on + strandat 1577.598 kb on + strand, within BWI76_RS07480at 1577.656 kb on - strand, within BWI76_RS07480at 1577.711 kb on + strand, within BWI76_RS07480at 1577.711 kb on + strand, within BWI76_RS07480at 1577.711 kb on + strand, within BWI76_RS07480at 1577.724 kb on + strand, within BWI76_RS07480at 1577.724 kb on + strand, within BWI76_RS07480at 1577.734 kb on + strand, within BWI76_RS07480at 1577.749 kb on + strand, within BWI76_RS07480

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
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1,574,702 + BWI76_RS07460 0.38 -0.1
1,574,900 + -1.3
1,574,947 - +0.3
1,575,250 - BWI76_RS07465 0.55 +0.0
1,575,306 + BWI76_RS07465 0.65 +0.4
1,575,514 - -1.6
1,575,575 - +0.4
1,575,835 - BWI76_RS07470 0.12 +0.4
1,575,968 + BWI76_RS07470 0.24 -1.0
1,576,364 + BWI76_RS07470 0.60 -0.6
1,576,519 - BWI76_RS07470 0.74 -1.1
1,576,693 + -1.4
1,576,703 + +0.6
1,576,801 + -0.4
1,576,969 + BWI76_RS07475 0.39 -0.8
1,577,368 + -0.9
1,577,598 + BWI76_RS07480 0.18 +0.3
1,577,656 - BWI76_RS07480 0.21 +0.7
1,577,711 + BWI76_RS07480 0.23 -2.2
1,577,711 + BWI76_RS07480 0.23 +0.2
1,577,711 + BWI76_RS07480 0.23 +0.3
1,577,724 + BWI76_RS07480 0.24 -0.3
1,577,724 + BWI76_RS07480 0.24 +0.2
1,577,734 + BWI76_RS07480 0.24 +0.5
1,577,749 + BWI76_RS07480 0.25 +0.3

Or see this region's nucleotide sequence