Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02355 and BWI76_RS02360 are separated by 134 nucleotides BWI76_RS02360 and BWI76_RS02365 are separated by 86 nucleotides BWI76_RS02365 and BWI76_RS02370 are separated by 23 nucleotides
BWI76_RS02355: BWI76_RS02355 - molecular chaperone GroEL, at 483,512 to 485,158
_RS02355
BWI76_RS02360: BWI76_RS02360 - hypothetical protein, at 485,293 to 485,646
_RS02360
BWI76_RS02365: BWI76_RS02365 - hypothetical protein, at 485,733 to 486,596
_RS02365
BWI76_RS02370: BWI76_RS02370 - colicin V synthesis protein, at 486,620 to 488,743
_RS02370
Position (kb)
485
486
487 Strain fitness (log2 ratio)
-2
-1
0
1 at 485.083 kb on + strand at 485.174 kb on - strand at 485.259 kb on - strand at 485.329 kb on + strand, within BWI76_RS02360 at 485.329 kb on + strand, within BWI76_RS02360 at 485.342 kb on - strand, within BWI76_RS02360 at 485.342 kb on - strand, within BWI76_RS02360 at 485.414 kb on - strand, within BWI76_RS02360 at 485.435 kb on - strand, within BWI76_RS02360 at 485.472 kb on + strand, within BWI76_RS02360 at 485.480 kb on - strand, within BWI76_RS02360 at 485.517 kb on + strand, within BWI76_RS02360 at 485.550 kb on + strand, within BWI76_RS02360 at 485.591 kb on + strand, within BWI76_RS02360 at 485.591 kb on + strand, within BWI76_RS02360 at 485.599 kb on - strand, within BWI76_RS02360 at 485.599 kb on - strand, within BWI76_RS02360 at 485.625 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.645 kb on + strand at 485.656 kb on + strand at 485.656 kb on + strand at 485.657 kb on + strand at 485.662 kb on + strand at 485.662 kb on + strand at 485.664 kb on - strand at 485.665 kb on - strand at 485.669 kb on + strand at 485.670 kb on - strand at 485.672 kb on + strand at 485.680 kb on + strand at 485.683 kb on - strand at 485.712 kb on - strand at 485.738 kb on - strand at 485.738 kb on - strand at 485.771 kb on + strand at 485.788 kb on + strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.861 kb on - strand, within BWI76_RS02365 at 485.954 kb on + strand, within BWI76_RS02365 at 485.989 kb on + strand at 486.097 kb on + strand, within BWI76_RS02365 at 486.105 kb on - strand, within BWI76_RS02365 at 486.105 kb on - strand, within BWI76_RS02365 at 486.112 kb on - strand, within BWI76_RS02365 at 486.193 kb on + strand at 486.201 kb on - strand, within BWI76_RS02365 at 486.203 kb on + strand, within BWI76_RS02365 at 486.313 kb on - strand, within BWI76_RS02365 at 486.407 kb on + strand, within BWI76_RS02365 at 486.415 kb on - strand, within BWI76_RS02365 at 486.415 kb on - strand, within BWI76_RS02365 at 486.420 kb on - strand, within BWI76_RS02365 at 486.470 kb on + strand, within BWI76_RS02365 at 486.471 kb on + strand, within BWI76_RS02365 at 486.479 kb on - strand, within BWI76_RS02365 at 486.479 kb on - strand, within BWI76_RS02365 at 486.553 kb on + strand at 486.634 kb on + strand at 486.636 kb on - strand at 486.641 kb on - strand at 486.642 kb on - strand at 486.642 kb on - strand at 486.642 kb on - strand at 486.696 kb on - strand at 486.706 kb on + strand at 486.714 kb on - strand at 486.724 kb on - strand at 486.865 kb on - strand, within BWI76_RS02370 at 486.865 kb on - strand, within BWI76_RS02370 at 487.038 kb on + strand, within BWI76_RS02370 at 487.038 kb on + strand, within BWI76_RS02370 at 487.093 kb on + strand, within BWI76_RS02370 at 487.093 kb on + strand, within BWI76_RS02370 at 487.101 kb on - strand, within BWI76_RS02370 at 487.104 kb on - strand, within BWI76_RS02370 at 487.154 kb on - strand, within BWI76_RS02370 at 487.154 kb on - strand, within BWI76_RS02370 at 487.154 kb on - strand, within BWI76_RS02370 at 487.156 kb on + strand, within BWI76_RS02370 at 487.164 kb on - strand, within BWI76_RS02370 at 487.194 kb on - strand, within BWI76_RS02370 at 487.286 kb on - strand, within BWI76_RS02370 at 487.301 kb on + strand, within BWI76_RS02370 at 487.321 kb on - strand at 487.321 kb on - strand, within BWI76_RS02370 at 487.321 kb on - strand at 487.407 kb on - strand, within BWI76_RS02370 at 487.451 kb on - strand, within BWI76_RS02370 at 487.462 kb on - strand, within BWI76_RS02370 at 487.468 kb on - strand, within BWI76_RS02370 at 487.550 kb on - strand, within BWI76_RS02370 at 487.564 kb on - strand, within BWI76_RS02370 at 487.578 kb on - strand, within BWI76_RS02370
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand, cultured with Sinorhizobium meliloti and Methylobacterium sp. UNC378MF remove 485,083 + -1.2 485,174 - -0.7 485,259 - -0.2 485,329 + BWI76_RS02360 0.10 +1.0 485,329 + BWI76_RS02360 0.10 -0.1 485,342 - BWI76_RS02360 0.14 -1.2 485,342 - BWI76_RS02360 0.14 +1.1 485,414 - BWI76_RS02360 0.34 -0.4 485,435 - BWI76_RS02360 0.40 -0.4 485,472 + BWI76_RS02360 0.51 +0.4 485,480 - BWI76_RS02360 0.53 +0.3 485,517 + BWI76_RS02360 0.63 +1.3 485,550 + BWI76_RS02360 0.73 +0.7 485,591 + BWI76_RS02360 0.84 -0.0 485,591 + BWI76_RS02360 0.84 +0.5 485,599 - BWI76_RS02360 0.86 -0.3 485,599 - BWI76_RS02360 0.86 +0.4 485,625 + -1.2 485,626 + -0.3 485,626 + +0.2 485,626 + -0.5 485,626 + -1.1 485,626 + -0.1 485,626 + +1.4 485,626 + -0.2 485,626 + -0.5 485,626 + +0.2 485,634 - -0.5 485,634 - +1.4 485,634 - +0.3 485,634 - -0.5 485,645 + -0.3 485,656 + -2.0 485,656 + -0.2 485,657 + -0.1 485,662 + -1.0 485,662 + +0.3 485,664 - +0.1 485,665 - +0.4 485,669 + -1.2 485,670 - +0.2 485,672 + -0.5 485,680 + +0.2 485,683 - -0.1 485,712 - +0.0 485,738 - +0.1 485,738 - +0.2 485,771 + +0.2 485,788 + -0.2 485,796 - +0.4 485,796 - -1.0 485,796 - -0.1 485,796 - -0.2 485,796 - -0.7 485,861 - BWI76_RS02365 0.15 -0.8 485,954 + BWI76_RS02365 0.26 -0.9 485,989 + -0.5 486,097 + BWI76_RS02365 0.42 -0.9 486,105 - BWI76_RS02365 0.43 +0.6 486,105 - BWI76_RS02365 0.43 -1.0 486,112 - BWI76_RS02365 0.44 -0.5 486,193 + +0.5 486,201 - BWI76_RS02365 0.54 -0.1 486,203 + BWI76_RS02365 0.54 -1.1 486,313 - BWI76_RS02365 0.67 +0.1 486,407 + BWI76_RS02365 0.78 -1.3 486,415 - BWI76_RS02365 0.79 +0.1 486,415 - BWI76_RS02365 0.79 +0.2 486,420 - BWI76_RS02365 0.80 -1.3 486,470 + BWI76_RS02365 0.85 -0.7 486,471 + BWI76_RS02365 0.85 -0.2 486,479 - BWI76_RS02365 0.86 -0.5 486,479 - BWI76_RS02365 0.86 -0.6 486,553 + +0.1 486,634 + +0.0 486,636 - -0.6 486,641 - -0.5 486,642 - +0.4 486,642 - +0.2 486,642 - -0.1 486,696 - +0.2 486,706 + -0.3 486,714 - -2.6 486,724 - +0.2 486,865 - BWI76_RS02370 0.12 +0.6 486,865 - BWI76_RS02370 0.12 -1.2 487,038 + BWI76_RS02370 0.20 +0.9 487,038 + BWI76_RS02370 0.20 -1.0 487,093 + BWI76_RS02370 0.22 -0.2 487,093 + BWI76_RS02370 0.22 +0.2 487,101 - BWI76_RS02370 0.23 -0.3 487,104 - BWI76_RS02370 0.23 +0.5 487,154 - BWI76_RS02370 0.25 -0.1 487,154 - BWI76_RS02370 0.25 -1.2 487,154 - BWI76_RS02370 0.25 -2.3 487,156 + BWI76_RS02370 0.25 -1.2 487,164 - BWI76_RS02370 0.26 -0.3 487,194 - BWI76_RS02370 0.27 -0.6 487,286 - BWI76_RS02370 0.31 +0.0 487,301 + BWI76_RS02370 0.32 +0.0 487,321 - +0.6 487,321 - BWI76_RS02370 0.33 +0.9 487,321 - +1.0 487,407 - BWI76_RS02370 0.37 -2.0 487,451 - BWI76_RS02370 0.39 -1.2 487,462 - BWI76_RS02370 0.40 +0.6 487,468 - BWI76_RS02370 0.40 -0.5 487,550 - BWI76_RS02370 0.44 -0.2 487,564 - BWI76_RS02370 0.44 -0.5 487,578 - BWI76_RS02370 0.45 +0.4
Or see this region's nucleotide sequence