Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20171 and SM_b20172 are separated by 13 nucleotides SM_b20172 and SM_b20173 are separated by 177 nucleotides SM_b20173 and SM_b20174 are separated by 78 nucleotides SM_b20174 and SM_b20175 overlap by 44 nucleotides
SM_b20171: SM_b20171 - carboxylesterase, at 189,530 to 190,372
_b20171
SM_b20172: SM_b20172 - cytochrome c protein, at 190,386 to 190,796
_b20172
SM_b20173: SM_b20173 - methanol dehydrogenase, large subunit, at 190,974 to 192,779
_b20173
SM_b20174: SM_b20174 - cytochrome c protein, at 192,858 to 193,388
_b20174
SM_b20175: SM_b20175 - hypothetical protein, at 193,345 to 194,211
_b20175
Position (kb)
190
191
192
193 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 190.133 kb on + strand, within SM_b20171 at 190.133 kb on + strand, within SM_b20171 at 190.133 kb on + strand, within SM_b20171 at 190.178 kb on - strand, within SM_b20171 at 190.304 kb on + strand at 190.307 kb on + strand at 190.307 kb on + strand at 190.615 kb on - strand, within SM_b20172 at 190.615 kb on - strand, within SM_b20172 at 190.620 kb on + strand, within SM_b20172 at 190.621 kb on - strand, within SM_b20172 at 190.621 kb on - strand, within SM_b20172 at 190.658 kb on + strand, within SM_b20172 at 190.658 kb on + strand, within SM_b20172 at 190.768 kb on + strand at 190.769 kb on - strand at 190.769 kb on - strand at 190.776 kb on + strand at 190.836 kb on - strand at 190.836 kb on - strand at 190.836 kb on - strand at 190.843 kb on + strand at 190.988 kb on + strand at 190.988 kb on + strand at 190.989 kb on - strand at 190.989 kb on - strand at 191.003 kb on + strand at 191.003 kb on + strand at 191.100 kb on + strand at 191.235 kb on + strand, within SM_b20173 at 191.235 kb on + strand, within SM_b20173 at 191.235 kb on + strand, within SM_b20173 at 191.236 kb on - strand, within SM_b20173 at 191.236 kb on - strand, within SM_b20173 at 191.243 kb on + strand, within SM_b20173 at 191.265 kb on + strand, within SM_b20173 at 191.308 kb on - strand, within SM_b20173 at 191.477 kb on + strand, within SM_b20173 at 191.477 kb on + strand, within SM_b20173 at 191.616 kb on + strand, within SM_b20173 at 191.616 kb on + strand, within SM_b20173 at 191.617 kb on - strand, within SM_b20173 at 191.640 kb on - strand, within SM_b20173 at 191.758 kb on - strand, within SM_b20173 at 191.764 kb on - strand, within SM_b20173 at 191.785 kb on + strand, within SM_b20173 at 191.787 kb on + strand, within SM_b20173 at 191.788 kb on - strand at 191.790 kb on + strand, within SM_b20173 at 191.790 kb on + strand, within SM_b20173 at 191.901 kb on + strand, within SM_b20173 at 191.901 kb on + strand at 191.931 kb on + strand, within SM_b20173 at 191.931 kb on + strand, within SM_b20173 at 191.932 kb on - strand, within SM_b20173 at 192.025 kb on - strand, within SM_b20173 at 192.025 kb on - strand, within SM_b20173 at 192.070 kb on - strand, within SM_b20173 at 192.126 kb on + strand, within SM_b20173 at 192.154 kb on - strand, within SM_b20173 at 192.264 kb on + strand, within SM_b20173 at 192.264 kb on + strand, within SM_b20173 at 192.264 kb on + strand, within SM_b20173 at 192.264 kb on + strand, within SM_b20173 at 192.265 kb on - strand, within SM_b20173 at 192.265 kb on - strand, within SM_b20173 at 192.294 kb on + strand, within SM_b20173 at 192.315 kb on + strand, within SM_b20173 at 192.315 kb on + strand, within SM_b20173 at 192.316 kb on - strand, within SM_b20173 at 192.316 kb on - strand, within SM_b20173 at 192.316 kb on - strand, within SM_b20173 at 192.316 kb on - strand, within SM_b20173 at 192.334 kb on - strand, within SM_b20173 at 192.334 kb on - strand, within SM_b20173 at 192.334 kb on - strand, within SM_b20173 at 192.334 kb on - strand, within SM_b20173 at 192.597 kb on + strand, within SM_b20173 at 192.597 kb on + strand, within SM_b20173 at 192.598 kb on - strand, within SM_b20173 at 192.598 kb on - strand, within SM_b20173 at 192.598 kb on - strand, within SM_b20173 at 192.618 kb on + strand at 192.618 kb on + strand at 192.715 kb on - strand at 192.732 kb on + strand at 192.732 kb on + strand at 192.732 kb on + strand at 192.733 kb on - strand at 192.778 kb on - strand at 192.816 kb on - strand at 192.834 kb on + strand at 192.834 kb on + strand at 192.908 kb on + strand at 192.908 kb on + strand at 192.981 kb on + strand, within SM_b20174 at 192.981 kb on + strand, within SM_b20174 at 192.984 kb on + strand, within SM_b20174 at 192.984 kb on + strand, within SM_b20174 at 192.985 kb on - strand, within SM_b20174 at 192.985 kb on - strand, within SM_b20174 at 193.065 kb on + strand, within SM_b20174 at 193.116 kb on + strand, within SM_b20174 at 193.116 kb on + strand, within SM_b20174 at 193.117 kb on - strand, within SM_b20174 at 193.155 kb on + strand, within SM_b20174 at 193.156 kb on - strand, within SM_b20174 at 193.159 kb on - strand, within SM_b20174 at 193.257 kb on + strand, within SM_b20174 at 193.258 kb on - strand, within SM_b20174 at 193.272 kb on + strand, within SM_b20174 at 193.272 kb on + strand, within SM_b20174 at 193.272 kb on + strand, within SM_b20174 at 193.272 kb on + strand, within SM_b20174 at 193.272 kb on + strand, within SM_b20174 at 193.272 kb on + strand, within SM_b20174 at 193.272 kb on + strand, within SM_b20174 at 193.272 kb on + strand, within SM_b20174 at 193.273 kb on - strand, within SM_b20174 at 193.273 kb on - strand, within SM_b20174 at 193.273 kb on - strand, within SM_b20174 at 193.273 kb on - strand, within SM_b20174 at 193.273 kb on - strand, within SM_b20174 at 193.273 kb on - strand, within SM_b20174 at 193.273 kb on - strand, within SM_b20174 at 193.386 kb on + strand at 193.386 kb on + strand at 193.461 kb on - strand, within SM_b20175 at 193.588 kb on + strand, within SM_b20175 at 193.589 kb on - strand, within SM_b20175 at 193.702 kb on + strand, within SM_b20175 at 193.703 kb on - strand, within SM_b20175 at 193.705 kb on + strand, within SM_b20175 at 193.705 kb on + strand, within SM_b20175 at 193.706 kb on - strand, within SM_b20175 at 193.706 kb on - strand, within SM_b20175 at 193.706 kb on - strand, within SM_b20175 at 193.706 kb on - strand, within SM_b20175 at 193.708 kb on - strand, within SM_b20175 at 193.708 kb on - strand, within SM_b20175 at 193.708 kb on - strand, within SM_b20175 at 193.708 kb on - strand, within SM_b20175 at 193.708 kb on - strand, within SM_b20175 at 193.708 kb on - strand, within SM_b20175 at 193.708 kb on - strand, within SM_b20175 at 193.717 kb on + strand, within SM_b20175 at 193.718 kb on - strand, within SM_b20175 at 193.718 kb on - strand, within SM_b20175 at 193.718 kb on - strand, within SM_b20175 at 193.718 kb on - strand, within SM_b20175
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 190,133 + SM_b20171 0.72 +0.5 190,133 + SM_b20171 0.72 +0.3 190,133 + SM_b20171 0.72 +0.6 190,178 - SM_b20171 0.77 -0.5 190,304 + -1.5 190,307 + +1.4 190,307 + -2.2 190,615 - SM_b20172 0.56 +1.1 190,615 - SM_b20172 0.56 -1.7 190,620 + SM_b20172 0.57 +0.2 190,621 - SM_b20172 0.57 +0.8 190,621 - SM_b20172 0.57 -0.4 190,658 + SM_b20172 0.66 -0.2 190,658 + SM_b20172 0.66 +0.1 190,768 + -0.5 190,769 - -0.2 190,769 - -0.2 190,776 + +0.3 190,836 - -0.1 190,836 - +0.1 190,836 - -0.8 190,843 + -0.2 190,988 + +2.4 190,988 + -0.0 190,989 - -0.1 190,989 - +0.8 191,003 + +0.2 191,003 + +0.0 191,100 + -0.3 191,235 + SM_b20173 0.14 +2.2 191,235 + SM_b20173 0.14 +0.7 191,235 + SM_b20173 0.14 -0.5 191,236 - SM_b20173 0.15 +0.7 191,236 - SM_b20173 0.15 -0.2 191,243 + SM_b20173 0.15 +0.0 191,265 + SM_b20173 0.16 +1.1 191,308 - SM_b20173 0.18 -2.0 191,477 + SM_b20173 0.28 -2.0 191,477 + SM_b20173 0.28 +0.4 191,616 + SM_b20173 0.36 +0.3 191,616 + SM_b20173 0.36 +1.1 191,617 - SM_b20173 0.36 +0.3 191,640 - SM_b20173 0.37 +0.2 191,758 - SM_b20173 0.43 +0.1 191,764 - SM_b20173 0.44 -0.4 191,785 + SM_b20173 0.45 -0.1 191,787 + SM_b20173 0.45 +0.2 191,788 - -1.4 191,790 + SM_b20173 0.45 +0.7 191,790 + SM_b20173 0.45 +0.7 191,901 + SM_b20173 0.51 +0.2 191,901 + +0.7 191,931 + SM_b20173 0.53 +1.7 191,931 + SM_b20173 0.53 -0.6 191,932 - SM_b20173 0.53 +0.2 192,025 - SM_b20173 0.58 -0.5 192,025 - SM_b20173 0.58 +0.1 192,070 - SM_b20173 0.61 -0.3 192,126 + SM_b20173 0.64 +0.2 192,154 - SM_b20173 0.65 +1.3 192,264 + SM_b20173 0.71 -1.0 192,264 + SM_b20173 0.71 +1.0 192,264 + SM_b20173 0.71 +0.1 192,264 + SM_b20173 0.71 +0.6 192,265 - SM_b20173 0.71 -0.2 192,265 - SM_b20173 0.71 -0.9 192,294 + SM_b20173 0.73 -0.3 192,315 + SM_b20173 0.74 -0.0 192,315 + SM_b20173 0.74 -0.6 192,316 - SM_b20173 0.74 -0.3 192,316 - SM_b20173 0.74 +1.1 192,316 - SM_b20173 0.74 +0.6 192,316 - SM_b20173 0.74 +0.3 192,334 - SM_b20173 0.75 +0.1 192,334 - SM_b20173 0.75 +1.0 192,334 - SM_b20173 0.75 +0.9 192,334 - SM_b20173 0.75 -0.3 192,597 + SM_b20173 0.90 -0.1 192,597 + SM_b20173 0.90 -0.2 192,598 - SM_b20173 0.90 -0.4 192,598 - SM_b20173 0.90 +0.3 192,598 - SM_b20173 0.90 +0.3 192,618 + -0.0 192,618 + +0.4 192,715 - -0.2 192,732 + +0.1 192,732 + -0.9 192,732 + -2.4 192,733 - -0.6 192,778 - +0.1 192,816 - +0.9 192,834 + +0.4 192,834 + +0.7 192,908 + -1.2 192,908 + +0.1 192,981 + SM_b20174 0.23 -0.9 192,981 + SM_b20174 0.23 -0.9 192,984 + SM_b20174 0.24 -0.1 192,984 + SM_b20174 0.24 -0.5 192,985 - SM_b20174 0.24 -1.2 192,985 - SM_b20174 0.24 +0.0 193,065 + SM_b20174 0.39 +1.3 193,116 + SM_b20174 0.49 -0.3 193,116 + SM_b20174 0.49 +0.2 193,117 - SM_b20174 0.49 +1.6 193,155 + SM_b20174 0.56 +0.3 193,156 - SM_b20174 0.56 -0.6 193,159 - SM_b20174 0.57 -0.3 193,257 + SM_b20174 0.75 +0.0 193,258 - SM_b20174 0.75 -0.9 193,272 + SM_b20174 0.78 -0.3 193,272 + SM_b20174 0.78 -0.4 193,272 + SM_b20174 0.78 -0.4 193,272 + SM_b20174 0.78 +0.2 193,272 + SM_b20174 0.78 +0.5 193,272 + SM_b20174 0.78 -1.1 193,272 + SM_b20174 0.78 -0.1 193,272 + SM_b20174 0.78 -1.0 193,273 - SM_b20174 0.78 -0.3 193,273 - SM_b20174 0.78 +0.1 193,273 - SM_b20174 0.78 -0.1 193,273 - SM_b20174 0.78 +0.3 193,273 - SM_b20174 0.78 -0.4 193,273 - SM_b20174 0.78 +1.1 193,273 - SM_b20174 0.78 +0.2 193,386 + -0.0 193,386 + -0.0 193,461 - SM_b20175 0.13 +0.0 193,588 + SM_b20175 0.28 -0.8 193,589 - SM_b20175 0.28 -1.1 193,702 + SM_b20175 0.41 -0.3 193,703 - SM_b20175 0.41 -0.6 193,705 + SM_b20175 0.42 +0.9 193,705 + SM_b20175 0.42 +0.9 193,706 - SM_b20175 0.42 -1.6 193,706 - SM_b20175 0.42 +1.0 193,706 - SM_b20175 0.42 -0.2 193,706 - SM_b20175 0.42 +0.9 193,708 - SM_b20175 0.42 +1.2 193,708 - SM_b20175 0.42 -0.4 193,708 - SM_b20175 0.42 -0.7 193,708 - SM_b20175 0.42 +0.6 193,708 - SM_b20175 0.42 +1.2 193,708 - SM_b20175 0.42 +0.1 193,708 - SM_b20175 0.42 +0.1 193,717 + SM_b20175 0.43 +0.4 193,718 - SM_b20175 0.43 -0.5 193,718 - SM_b20175 0.43 -0.7 193,718 - SM_b20175 0.43 -0.6 193,718 - SM_b20175 0.43 -0.1
Or see this region's nucleotide sequence