Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17250 and BWI76_RS17255 are separated by 176 nucleotides BWI76_RS17255 and BWI76_RS17260 are separated by 144 nucleotides BWI76_RS17260 and BWI76_RS17265 are separated by 93 nucleotides BWI76_RS17265 and BWI76_RS17270 are separated by 134 nucleotides
BWI76_RS17250: BWI76_RS17250 - bifunctional acetaldehyde-CoA/alcohol dehydrogenase, at 3,574,176 to 3,576,851
_RS17250
BWI76_RS17255: BWI76_RS17255 - thymidine kinase, at 3,577,028 to 3,577,645
_RS17255
BWI76_RS17260: BWI76_RS17260 - hypothetical protein, at 3,577,790 to 3,578,108
_RS17260
BWI76_RS17265: BWI76_RS17265 - DNA-binding protein, at 3,578,202 to 3,578,609
_RS17265
BWI76_RS17270: BWI76_RS17270 - UTP--glucose-1-phosphate uridylyltransferase, at 3,578,744 to 3,579,646
_RS17270
Position (kb)
3577
3578
3579 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3576.797 kb on - strand at 3576.815 kb on + strand at 3576.823 kb on - strand at 3577.054 kb on - strand at 3577.203 kb on - strand, within BWI76_RS17255 at 3577.203 kb on - strand, within BWI76_RS17255 at 3577.301 kb on - strand, within BWI76_RS17255 at 3577.313 kb on - strand, within BWI76_RS17255 at 3577.338 kb on + strand, within BWI76_RS17255 at 3577.338 kb on + strand at 3577.338 kb on + strand, within BWI76_RS17255 at 3577.338 kb on + strand, within BWI76_RS17255 at 3577.339 kb on + strand, within BWI76_RS17255 at 3577.346 kb on - strand, within BWI76_RS17255 at 3577.346 kb on - strand, within BWI76_RS17255 at 3577.365 kb on - strand, within BWI76_RS17255 at 3577.368 kb on - strand, within BWI76_RS17255 at 3577.376 kb on - strand, within BWI76_RS17255 at 3577.382 kb on + strand, within BWI76_RS17255 at 3577.421 kb on - strand, within BWI76_RS17255 at 3577.421 kb on - strand, within BWI76_RS17255 at 3577.423 kb on - strand, within BWI76_RS17255 at 3577.425 kb on + strand, within BWI76_RS17255 at 3577.433 kb on - strand, within BWI76_RS17255 at 3577.463 kb on - strand, within BWI76_RS17255 at 3577.473 kb on + strand, within BWI76_RS17255 at 3577.481 kb on - strand, within BWI76_RS17255 at 3577.536 kb on + strand, within BWI76_RS17255 at 3577.536 kb on + strand, within BWI76_RS17255 at 3577.536 kb on + strand, within BWI76_RS17255 at 3577.536 kb on + strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.544 kb on - strand, within BWI76_RS17255 at 3577.589 kb on - strand at 3577.593 kb on + strand at 3577.599 kb on - strand at 3577.602 kb on - strand at 3577.623 kb on - strand at 3577.659 kb on - strand at 3577.723 kb on + strand at 3577.728 kb on + strand at 3577.729 kb on - strand at 3577.740 kb on - strand at 3577.757 kb on - strand at 3577.759 kb on - strand at 3577.759 kb on - strand at 3577.773 kb on - strand at 3577.797 kb on + strand at 3577.802 kb on - strand at 3577.805 kb on - strand at 3577.816 kb on - strand at 3577.825 kb on + strand, within BWI76_RS17260 at 3577.840 kb on - strand, within BWI76_RS17260 at 3577.848 kb on - strand, within BWI76_RS17260 at 3577.848 kb on - strand, within BWI76_RS17260 at 3577.915 kb on - strand, within BWI76_RS17260 at 3577.945 kb on + strand, within BWI76_RS17260 at 3577.963 kb on - strand, within BWI76_RS17260 at 3577.963 kb on - strand, within BWI76_RS17260 at 3577.992 kb on - strand, within BWI76_RS17260 at 3578.014 kb on - strand, within BWI76_RS17260 at 3578.118 kb on + strand at 3578.119 kb on - strand at 3578.123 kb on + strand at 3578.123 kb on + strand at 3578.522 kb on + strand, within BWI76_RS17265 at 3578.673 kb on - strand at 3578.747 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads remove 3,576,797 - -1.1 3,576,815 + -0.5 3,576,823 - -0.6 3,577,054 - +0.2 3,577,203 - BWI76_RS17255 0.28 -0.3 3,577,203 - BWI76_RS17255 0.28 -0.2 3,577,301 - BWI76_RS17255 0.44 -0.1 3,577,313 - BWI76_RS17255 0.46 +0.3 3,577,338 + BWI76_RS17255 0.50 -1.7 3,577,338 + -1.4 3,577,338 + BWI76_RS17255 0.50 -0.7 3,577,338 + BWI76_RS17255 0.50 -0.1 3,577,339 + BWI76_RS17255 0.50 +0.3 3,577,346 - BWI76_RS17255 0.51 -0.2 3,577,346 - BWI76_RS17255 0.51 +0.7 3,577,365 - BWI76_RS17255 0.55 -1.0 3,577,368 - BWI76_RS17255 0.55 -0.5 3,577,376 - BWI76_RS17255 0.56 -0.8 3,577,382 + BWI76_RS17255 0.57 -0.3 3,577,421 - BWI76_RS17255 0.64 -1.2 3,577,421 - BWI76_RS17255 0.64 -0.6 3,577,423 - BWI76_RS17255 0.64 -0.5 3,577,425 + BWI76_RS17255 0.64 -0.0 3,577,433 - BWI76_RS17255 0.66 -0.1 3,577,463 - BWI76_RS17255 0.70 -1.4 3,577,473 + BWI76_RS17255 0.72 +0.4 3,577,481 - BWI76_RS17255 0.73 -0.3 3,577,536 + BWI76_RS17255 0.82 -0.6 3,577,536 + BWI76_RS17255 0.82 -0.6 3,577,536 + BWI76_RS17255 0.82 -1.2 3,577,536 + BWI76_RS17255 0.82 -0.7 3,577,544 - BWI76_RS17255 0.83 -0.8 3,577,544 - BWI76_RS17255 0.83 +0.4 3,577,544 - BWI76_RS17255 0.83 -0.4 3,577,544 - BWI76_RS17255 0.83 -0.3 3,577,544 - BWI76_RS17255 0.83 -0.3 3,577,544 - BWI76_RS17255 0.83 -1.0 3,577,544 - BWI76_RS17255 0.83 -0.5 3,577,544 - BWI76_RS17255 0.83 +0.0 3,577,544 - BWI76_RS17255 0.83 -0.6 3,577,544 - BWI76_RS17255 0.83 -0.7 3,577,544 - BWI76_RS17255 0.83 -0.8 3,577,544 - BWI76_RS17255 0.83 +0.8 3,577,589 - -0.2 3,577,593 + -0.6 3,577,599 - +1.0 3,577,602 - -0.1 3,577,623 - -0.7 3,577,659 - -0.0 3,577,723 + -1.7 3,577,728 + -0.6 3,577,729 - -1.7 3,577,740 - -0.9 3,577,757 - -0.2 3,577,759 - -0.0 3,577,759 - +0.2 3,577,773 - -1.9 3,577,797 + -2.3 3,577,802 - -0.4 3,577,805 - -0.3 3,577,816 - +0.0 3,577,825 + BWI76_RS17260 0.11 -0.0 3,577,840 - BWI76_RS17260 0.16 -0.9 3,577,848 - BWI76_RS17260 0.18 -0.5 3,577,848 - BWI76_RS17260 0.18 +0.8 3,577,915 - BWI76_RS17260 0.39 +0.5 3,577,945 + BWI76_RS17260 0.49 -1.0 3,577,963 - BWI76_RS17260 0.54 -0.3 3,577,963 - BWI76_RS17260 0.54 +0.3 3,577,992 - BWI76_RS17260 0.63 +0.7 3,578,014 - BWI76_RS17260 0.70 -0.0 3,578,118 + +0.4 3,578,119 - +1.9 3,578,123 + -0.4 3,578,123 + -0.8 3,578,522 + BWI76_RS17265 0.78 -2.3 3,578,673 - -1.9 3,578,747 - -0.6
Or see this region's nucleotide sequence