Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17260

Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17250 and BWI76_RS17255 are separated by 176 nucleotidesBWI76_RS17255 and BWI76_RS17260 are separated by 144 nucleotidesBWI76_RS17260 and BWI76_RS17265 are separated by 93 nucleotidesBWI76_RS17265 and BWI76_RS17270 are separated by 134 nucleotides BWI76_RS17250: BWI76_RS17250 - bifunctional acetaldehyde-CoA/alcohol dehydrogenase, at 3,574,176 to 3,576,851 _RS17250 BWI76_RS17255: BWI76_RS17255 - thymidine kinase, at 3,577,028 to 3,577,645 _RS17255 BWI76_RS17260: BWI76_RS17260 - hypothetical protein, at 3,577,790 to 3,578,108 _RS17260 BWI76_RS17265: BWI76_RS17265 - DNA-binding protein, at 3,578,202 to 3,578,609 _RS17265 BWI76_RS17270: BWI76_RS17270 - UTP--glucose-1-phosphate uridylyltransferase, at 3,578,744 to 3,579,646 _RS17270 Position (kb) 3577 3578 3579Strain fitness (log2 ratio) -2 -1 0 1 2at 3576.797 kb on - strandat 3576.815 kb on + strandat 3576.823 kb on - strandat 3577.054 kb on - strandat 3577.203 kb on - strand, within BWI76_RS17255at 3577.203 kb on - strand, within BWI76_RS17255at 3577.301 kb on - strand, within BWI76_RS17255at 3577.313 kb on - strand, within BWI76_RS17255at 3577.338 kb on + strand, within BWI76_RS17255at 3577.338 kb on + strandat 3577.338 kb on + strand, within BWI76_RS17255at 3577.338 kb on + strand, within BWI76_RS17255at 3577.339 kb on + strand, within BWI76_RS17255at 3577.346 kb on - strand, within BWI76_RS17255at 3577.346 kb on - strand, within BWI76_RS17255at 3577.365 kb on - strand, within BWI76_RS17255at 3577.368 kb on - strand, within BWI76_RS17255at 3577.376 kb on - strand, within BWI76_RS17255at 3577.382 kb on + strand, within BWI76_RS17255at 3577.421 kb on - strand, within BWI76_RS17255at 3577.421 kb on - strand, within BWI76_RS17255at 3577.423 kb on - strand, within BWI76_RS17255at 3577.425 kb on + strand, within BWI76_RS17255at 3577.433 kb on - strand, within BWI76_RS17255at 3577.463 kb on - strand, within BWI76_RS17255at 3577.473 kb on + strand, within BWI76_RS17255at 3577.481 kb on - strand, within BWI76_RS17255at 3577.536 kb on + strand, within BWI76_RS17255at 3577.536 kb on + strand, within BWI76_RS17255at 3577.536 kb on + strand, within BWI76_RS17255at 3577.536 kb on + strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.544 kb on - strand, within BWI76_RS17255at 3577.589 kb on - strandat 3577.593 kb on + strandat 3577.599 kb on - strandat 3577.602 kb on - strandat 3577.623 kb on - strandat 3577.659 kb on - strandat 3577.723 kb on + strandat 3577.728 kb on + strandat 3577.729 kb on - strandat 3577.740 kb on - strandat 3577.757 kb on - strandat 3577.759 kb on - strandat 3577.759 kb on - strandat 3577.773 kb on - strandat 3577.797 kb on + strandat 3577.802 kb on - strandat 3577.805 kb on - strandat 3577.816 kb on - strandat 3577.825 kb on + strand, within BWI76_RS17260at 3577.840 kb on - strand, within BWI76_RS17260at 3577.848 kb on - strand, within BWI76_RS17260at 3577.848 kb on - strand, within BWI76_RS17260at 3577.915 kb on - strand, within BWI76_RS17260at 3577.945 kb on + strand, within BWI76_RS17260at 3577.963 kb on - strand, within BWI76_RS17260at 3577.963 kb on - strand, within BWI76_RS17260at 3577.992 kb on - strand, within BWI76_RS17260at 3578.014 kb on - strand, within BWI76_RS17260at 3578.118 kb on + strandat 3578.119 kb on - strandat 3578.123 kb on + strandat 3578.123 kb on + strandat 3578.522 kb on + strand, within BWI76_RS17265at 3578.673 kb on - strandat 3578.747 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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3,576,797 - -1.1
3,576,815 + -0.5
3,576,823 - -0.6
3,577,054 - +0.2
3,577,203 - BWI76_RS17255 0.28 -0.3
3,577,203 - BWI76_RS17255 0.28 -0.2
3,577,301 - BWI76_RS17255 0.44 -0.1
3,577,313 - BWI76_RS17255 0.46 +0.3
3,577,338 + BWI76_RS17255 0.50 -1.7
3,577,338 + -1.4
3,577,338 + BWI76_RS17255 0.50 -0.7
3,577,338 + BWI76_RS17255 0.50 -0.1
3,577,339 + BWI76_RS17255 0.50 +0.3
3,577,346 - BWI76_RS17255 0.51 -0.2
3,577,346 - BWI76_RS17255 0.51 +0.7
3,577,365 - BWI76_RS17255 0.55 -1.0
3,577,368 - BWI76_RS17255 0.55 -0.5
3,577,376 - BWI76_RS17255 0.56 -0.8
3,577,382 + BWI76_RS17255 0.57 -0.3
3,577,421 - BWI76_RS17255 0.64 -1.2
3,577,421 - BWI76_RS17255 0.64 -0.6
3,577,423 - BWI76_RS17255 0.64 -0.5
3,577,425 + BWI76_RS17255 0.64 -0.0
3,577,433 - BWI76_RS17255 0.66 -0.1
3,577,463 - BWI76_RS17255 0.70 -1.4
3,577,473 + BWI76_RS17255 0.72 +0.4
3,577,481 - BWI76_RS17255 0.73 -0.3
3,577,536 + BWI76_RS17255 0.82 -0.6
3,577,536 + BWI76_RS17255 0.82 -0.6
3,577,536 + BWI76_RS17255 0.82 -1.2
3,577,536 + BWI76_RS17255 0.82 -0.7
3,577,544 - BWI76_RS17255 0.83 -0.8
3,577,544 - BWI76_RS17255 0.83 +0.4
3,577,544 - BWI76_RS17255 0.83 -0.4
3,577,544 - BWI76_RS17255 0.83 -0.3
3,577,544 - BWI76_RS17255 0.83 -0.3
3,577,544 - BWI76_RS17255 0.83 -1.0
3,577,544 - BWI76_RS17255 0.83 -0.5
3,577,544 - BWI76_RS17255 0.83 +0.0
3,577,544 - BWI76_RS17255 0.83 -0.6
3,577,544 - BWI76_RS17255 0.83 -0.7
3,577,544 - BWI76_RS17255 0.83 -0.8
3,577,544 - BWI76_RS17255 0.83 +0.8
3,577,589 - -0.2
3,577,593 + -0.6
3,577,599 - +1.0
3,577,602 - -0.1
3,577,623 - -0.7
3,577,659 - -0.0
3,577,723 + -1.7
3,577,728 + -0.6
3,577,729 - -1.7
3,577,740 - -0.9
3,577,757 - -0.2
3,577,759 - -0.0
3,577,759 - +0.2
3,577,773 - -1.9
3,577,797 + -2.3
3,577,802 - -0.4
3,577,805 - -0.3
3,577,816 - +0.0
3,577,825 + BWI76_RS17260 0.11 -0.0
3,577,840 - BWI76_RS17260 0.16 -0.9
3,577,848 - BWI76_RS17260 0.18 -0.5
3,577,848 - BWI76_RS17260 0.18 +0.8
3,577,915 - BWI76_RS17260 0.39 +0.5
3,577,945 + BWI76_RS17260 0.49 -1.0
3,577,963 - BWI76_RS17260 0.54 -0.3
3,577,963 - BWI76_RS17260 0.54 +0.3
3,577,992 - BWI76_RS17260 0.63 +0.7
3,578,014 - BWI76_RS17260 0.70 -0.0
3,578,118 + +0.4
3,578,119 - +1.9
3,578,123 + -0.4
3,578,123 + -0.8
3,578,522 + BWI76_RS17265 0.78 -2.3
3,578,673 - -1.9
3,578,747 - -0.6

Or see this region's nucleotide sequence