Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11515 and BWI76_RS11520 are separated by 42 nucleotides BWI76_RS11520 and BWI76_RS11525 are separated by 60 nucleotides BWI76_RS11525 and BWI76_RS11530 are separated by 100 nucleotides BWI76_RS11530 and BWI76_RS11535 are separated by 324 nucleotides
BWI76_RS11515: BWI76_RS11515 - peptide-methionine (R)-S-oxide reductase, at 2,423,817 to 2,424,230
_RS11515
BWI76_RS11520: BWI76_RS11520 - hypothetical protein, at 2,424,273 to 2,424,548
_RS11520
BWI76_RS11525: BWI76_RS11525 - HNH endonuclease, at 2,424,609 to 2,424,953
_RS11525
BWI76_RS11530: BWI76_RS11530 - chitinase, at 2,425,054 to 2,426,307
_RS11530
BWI76_RS11535: BWI76_RS11535 - putative oxidoreductase, Zn-dependent and NAD(P)-binding protein, at 2,426,632 to 2,427,708
_RS11535
Position (kb)
2425
2426
2427 Strain fitness (log2 ratio)
-1
0
1 at 2424.443 kb on + strand, within BWI76_RS11520 at 2424.573 kb on + strand at 2424.759 kb on + strand, within BWI76_RS11525 at 2424.772 kb on - strand, within BWI76_RS11525 at 2425.060 kb on - strand at 2425.203 kb on + strand, within BWI76_RS11530 at 2425.553 kb on + strand, within BWI76_RS11530 at 2425.558 kb on + strand, within BWI76_RS11530 at 2425.566 kb on - strand, within BWI76_RS11530 at 2425.605 kb on + strand, within BWI76_RS11530 at 2425.646 kb on - strand, within BWI76_RS11530 at 2425.753 kb on + strand, within BWI76_RS11530 at 2425.924 kb on + strand, within BWI76_RS11530 at 2426.128 kb on - strand, within BWI76_RS11530 at 2426.467 kb on + strand at 2427.190 kb on - strand, within BWI76_RS11535
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads remove 2,424,443 + BWI76_RS11520 0.62 -0.0 2,424,573 + +0.9 2,424,759 + BWI76_RS11525 0.43 +0.1 2,424,772 - BWI76_RS11525 0.47 -0.4 2,425,060 - -0.6 2,425,203 + BWI76_RS11530 0.12 +0.3 2,425,553 + BWI76_RS11530 0.40 +0.7 2,425,558 + BWI76_RS11530 0.40 +0.4 2,425,566 - BWI76_RS11530 0.41 -0.3 2,425,605 + BWI76_RS11530 0.44 +1.5 2,425,646 - BWI76_RS11530 0.47 +0.3 2,425,753 + BWI76_RS11530 0.56 +0.1 2,425,924 + BWI76_RS11530 0.69 +0.4 2,426,128 - BWI76_RS11530 0.86 +1.1 2,426,467 + -0.5 2,427,190 - BWI76_RS11535 0.52 -0.2
Or see this region's nucleotide sequence