Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05220

Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05215 and BWI76_RS05220 are separated by 146 nucleotidesBWI76_RS05220 and BWI76_RS05225 are separated by 205 nucleotidesBWI76_RS05225 and BWI76_RS05230 are separated by 226 nucleotides BWI76_RS05215: BWI76_RS05215 - elongation factor Ts, at 1,098,546 to 1,099,397 _RS05215 BWI76_RS05220: BWI76_RS05220 - UMP kinase, at 1,099,544 to 1,100,269 _RS05220 BWI76_RS05225: BWI76_RS05225 - ribosome-recycling factor, at 1,100,475 to 1,101,032 _RS05225 BWI76_RS05230: BWI76_RS05230 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase, at 1,101,259 to 1,102,458 _RS05230 Position (kb) 1099 1100 1101Strain fitness (log2 ratio) -1 0 1at 1101.050 kb on + strandat 1101.058 kb on - strandat 1101.058 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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1,101,050 + +1.1
1,101,058 - -0.3
1,101,058 - -0.4

Or see this region's nucleotide sequence