Experiment: MoLS4 with L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mgtE and nifB are separated by 449 nucleotides nifB and nifN are separated by 12 nucleotides
DVUA0006: mgtE - magnesium transporter MgtE, putative (TIGR), at 7,456 to 8,451
mgtE
DVUA0007: nifB - nitrogenase cofactor biosynthesis protein NifB (TIGR), at 8,901 to 10,445
nifB
DVUA0008: nifN - nitrogenase molybdenum-iron cofactor biosynthesis protein NifN, putative (TIGR), at 10,458 to 11,972
nifN
Position (kb)
8
9
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 8.000 kb on - strand, within mgtE at 8.071 kb on + strand, within mgtE at 8.072 kb on - strand, within mgtE at 8.152 kb on + strand, within mgtE at 8.160 kb on - strand, within mgtE at 8.251 kb on - strand, within mgtE at 8.404 kb on - strand at 8.643 kb on - strand at 8.695 kb on + strand at 8.741 kb on + strand at 8.762 kb on + strand at 8.785 kb on + strand at 8.841 kb on + strand at 8.849 kb on - strand at 8.931 kb on - strand at 8.931 kb on - strand at 8.933 kb on - strand at 9.042 kb on - strand at 9.042 kb on - strand at 9.104 kb on + strand, within nifB at 9.124 kb on - strand, within nifB at 9.142 kb on - strand, within nifB at 9.178 kb on - strand, within nifB at 9.261 kb on - strand, within nifB at 9.313 kb on + strand, within nifB at 9.330 kb on + strand, within nifB at 9.338 kb on - strand, within nifB at 9.340 kb on - strand, within nifB at 9.340 kb on - strand, within nifB at 9.363 kb on - strand, within nifB at 9.448 kb on + strand, within nifB at 9.521 kb on - strand, within nifB at 9.548 kb on - strand, within nifB at 9.561 kb on - strand, within nifB at 9.612 kb on - strand, within nifB at 9.633 kb on + strand, within nifB at 9.641 kb on + strand, within nifB at 9.663 kb on - strand, within nifB at 9.669 kb on + strand, within nifB at 9.674 kb on + strand, within nifB at 9.698 kb on + strand, within nifB at 9.698 kb on + strand, within nifB at 9.706 kb on - strand, within nifB at 9.737 kb on - strand, within nifB at 9.744 kb on - strand, within nifB at 9.795 kb on - strand, within nifB at 9.814 kb on + strand, within nifB at 9.871 kb on + strand, within nifB at 9.879 kb on - strand, within nifB at 9.879 kb on - strand, within nifB at 9.991 kb on + strand, within nifB at 9.991 kb on - strand, within nifB at 9.998 kb on + strand, within nifB at 9.999 kb on - strand, within nifB at 9.999 kb on - strand, within nifB at 10.084 kb on + strand, within nifB at 10.097 kb on - strand, within nifB at 10.153 kb on - strand, within nifB at 10.262 kb on + strand, within nifB at 10.275 kb on - strand, within nifB at 10.277 kb on - strand, within nifB at 10.370 kb on + strand at 10.426 kb on - strand at 10.443 kb on - strand at 10.509 kb on + strand at 10.532 kb on + strand at 10.743 kb on + strand, within nifN at 10.744 kb on - strand, within nifN at 10.789 kb on - strand, within nifN at 10.806 kb on + strand, within nifN at 10.818 kb on + strand, within nifN at 10.826 kb on - strand, within nifN at 10.843 kb on + strand, within nifN at 10.843 kb on + strand, within nifN at 10.846 kb on - strand, within nifN at 10.859 kb on - strand, within nifN at 10.873 kb on + strand, within nifN at 10.997 kb on + strand, within nifN at 11.011 kb on + strand, within nifN at 11.037 kb on - strand, within nifN at 11.038 kb on + strand, within nifN at 11.041 kb on - strand, within nifN at 11.284 kb on - strand, within nifN at 11.293 kb on + strand, within nifN at 11.293 kb on + strand, within nifN at 11.327 kb on - strand, within nifN at 11.338 kb on + strand, within nifN at 11.347 kb on - strand, within nifN at 11.382 kb on - strand, within nifN at 11.394 kb on + strand, within nifN at 11.417 kb on - strand, within nifN
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Tryptophan (N) remove 8,000 - mgtE DVUA0006 0.55 -0.3 8,071 + mgtE DVUA0006 0.62 +0.1 8,072 - mgtE DVUA0006 0.62 -0.1 8,152 + mgtE DVUA0006 0.70 +1.5 8,160 - mgtE DVUA0006 0.71 -0.2 8,251 - mgtE DVUA0006 0.80 +0.5 8,404 - +0.0 8,643 - -0.3 8,695 + -0.3 8,741 + -0.5 8,762 + -0.2 8,785 + -0.3 8,841 + -0.5 8,849 - -0.2 8,931 - -1.2 8,931 - -0.5 8,933 - -1.7 9,042 - -2.6 9,042 - -0.8 9,104 + nifB DVUA0007 0.13 -0.2 9,124 - nifB DVUA0007 0.14 -1.4 9,142 - nifB DVUA0007 0.16 -1.6 9,178 - nifB DVUA0007 0.18 -1.2 9,261 - nifB DVUA0007 0.23 +0.8 9,313 + nifB DVUA0007 0.27 -1.6 9,330 + nifB DVUA0007 0.28 -1.1 9,338 - nifB DVUA0007 0.28 -1.4 9,340 - nifB DVUA0007 0.28 -1.3 9,340 - nifB DVUA0007 0.28 -1.8 9,363 - nifB DVUA0007 0.30 -2.8 9,448 + nifB DVUA0007 0.35 -2.7 9,521 - nifB DVUA0007 0.40 -0.9 9,548 - nifB DVUA0007 0.42 -1.6 9,561 - nifB DVUA0007 0.43 -0.4 9,612 - nifB DVUA0007 0.46 -1.4 9,633 + nifB DVUA0007 0.47 -1.6 9,641 + nifB DVUA0007 0.48 -1.3 9,663 - nifB DVUA0007 0.49 -1.8 9,669 + nifB DVUA0007 0.50 -1.4 9,674 + nifB DVUA0007 0.50 -0.2 9,698 + nifB DVUA0007 0.52 +0.5 9,698 + nifB DVUA0007 0.52 -1.3 9,706 - nifB DVUA0007 0.52 -0.7 9,737 - nifB DVUA0007 0.54 -0.2 9,744 - nifB DVUA0007 0.55 -2.5 9,795 - nifB DVUA0007 0.58 -1.7 9,814 + nifB DVUA0007 0.59 -2.5 9,871 + nifB DVUA0007 0.63 -0.9 9,879 - nifB DVUA0007 0.63 -1.3 9,879 - nifB DVUA0007 0.63 -2.5 9,991 + nifB DVUA0007 0.71 -1.2 9,991 - nifB DVUA0007 0.71 -1.7 9,998 + nifB DVUA0007 0.71 -1.0 9,999 - nifB DVUA0007 0.71 -1.5 9,999 - nifB DVUA0007 0.71 -1.5 10,084 + nifB DVUA0007 0.77 -1.9 10,097 - nifB DVUA0007 0.77 -1.2 10,153 - nifB DVUA0007 0.81 -0.8 10,262 + nifB DVUA0007 0.88 -1.8 10,275 - nifB DVUA0007 0.89 -0.9 10,277 - nifB DVUA0007 0.89 -2.1 10,370 + -1.0 10,426 - -1.7 10,443 - -1.5 10,509 + -0.9 10,532 + -1.3 10,743 + nifN DVUA0008 0.19 -1.1 10,744 - nifN DVUA0008 0.19 -1.8 10,789 - nifN DVUA0008 0.22 -1.8 10,806 + nifN DVUA0008 0.23 -1.8 10,818 + nifN DVUA0008 0.24 -0.8 10,826 - nifN DVUA0008 0.24 -1.1 10,843 + nifN DVUA0008 0.25 +0.5 10,843 + nifN DVUA0008 0.25 -3.5 10,846 - nifN DVUA0008 0.26 -1.7 10,859 - nifN DVUA0008 0.26 -1.5 10,873 + nifN DVUA0008 0.27 -0.3 10,997 + nifN DVUA0008 0.36 -1.3 11,011 + nifN DVUA0008 0.37 +1.5 11,037 - nifN DVUA0008 0.38 -3.7 11,038 + nifN DVUA0008 0.38 -1.8 11,041 - nifN DVUA0008 0.38 -2.2 11,284 - nifN DVUA0008 0.55 -1.3 11,293 + nifN DVUA0008 0.55 -2.7 11,293 + nifN DVUA0008 0.55 -1.5 11,327 - nifN DVUA0008 0.57 -0.9 11,338 + nifN DVUA0008 0.58 +1.0 11,347 - nifN DVUA0008 0.59 -1.5 11,382 - nifN DVUA0008 0.61 -2.2 11,394 + nifN DVUA0008 0.62 -2.4 11,417 - nifN DVUA0008 0.63 -1.9
Or see this region's nucleotide sequence