Experiment: MoLS4 with L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnpB and DVU1455 are separated by 12 nucleotides DVU1455 and DVU1456 are separated by 40 nucleotides DVU1456 and tRNA-Met are separated by 119 nucleotides tRNA-Met and trxB are separated by 48 nucleotides
DvrnpB1: rnpB - RnpB RNA: catalytic subunit of RNase P (VIMSS-AUTO), at 1,533,841 to 1,534,196
rnpB
DVU1455: DVU1455 - conserved hypothetical protein (TIGR), at 1,534,209 to 1,534,952
DVU1455
DVU1456: DVU1456 - NIF3 family protein (TIGR), at 1,534,993 to 1,536,003
DVU1456
DVU_tRNA-Met-2: tRNA-Met - tRNA with anticodon CAT for Met (VIMSS-AUTO), at 1,536,123 to 1,536,198
tRNA-Met
DVU1457: trxB - thioredoxin reductase, putative (TIGR), at 1,536,247 to 1,537,176
trxB
Position (kb)
1534
1535
1536
1537 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1534.231 kb on + strand at 1534.383 kb on + strand, within DVU1455 at 1534.549 kb on - strand, within DVU1455 at 1534.580 kb on - strand, within DVU1455 at 1534.835 kb on + strand, within DVU1455 at 1534.835 kb on + strand, within DVU1455 at 1534.843 kb on - strand, within DVU1455 at 1534.874 kb on - strand, within DVU1455 at 1534.908 kb on + strand at 1534.934 kb on + strand at 1535.032 kb on - strand at 1535.084 kb on - strand at 1535.357 kb on + strand, within DVU1456 at 1535.482 kb on - strand, within DVU1456 at 1535.539 kb on + strand, within DVU1456 at 1535.557 kb on - strand, within DVU1456 at 1535.575 kb on + strand, within DVU1456 at 1535.575 kb on - strand, within DVU1456 at 1535.582 kb on - strand, within DVU1456 at 1535.745 kb on - strand, within DVU1456 at 1535.764 kb on - strand, within DVU1456 at 1535.776 kb on - strand, within DVU1456 at 1536.073 kb on - strand at 1536.275 kb on - strand at 1536.294 kb on - strand at 1536.296 kb on + strand at 1536.302 kb on + strand at 1536.315 kb on - strand at 1536.334 kb on - strand at 1536.404 kb on - strand, within trxB at 1536.415 kb on - strand, within trxB at 1536.558 kb on + strand, within trxB at 1536.602 kb on - strand, within trxB at 1536.634 kb on + strand, within trxB at 1536.691 kb on + strand, within trxB at 1536.699 kb on + strand, within trxB at 1536.699 kb on - strand, within trxB at 1536.749 kb on - strand, within trxB at 1536.776 kb on + strand, within trxB at 1536.804 kb on - strand, within trxB at 1536.805 kb on + strand, within trxB at 1536.891 kb on + strand, within trxB at 1536.965 kb on - strand, within trxB
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Tryptophan (N) remove 1,534,231 + -1.1 1,534,383 + DVU1455 0.23 -0.9 1,534,549 - DVU1455 0.46 +1.5 1,534,580 - DVU1455 0.50 +1.1 1,534,835 + DVU1455 0.84 +0.9 1,534,835 + DVU1455 0.84 +1.1 1,534,843 - DVU1455 0.85 +0.3 1,534,874 - DVU1455 0.89 -1.6 1,534,908 + -0.5 1,534,934 + +0.7 1,535,032 - -1.5 1,535,084 - +0.7 1,535,357 + DVU1456 0.36 -1.3 1,535,482 - DVU1456 0.48 +1.1 1,535,539 + DVU1456 0.54 -0.8 1,535,557 - DVU1456 0.56 -1.6 1,535,575 + DVU1456 0.58 -1.3 1,535,575 - DVU1456 0.58 -0.1 1,535,582 - DVU1456 0.58 -1.8 1,535,745 - DVU1456 0.74 +2.1 1,535,764 - DVU1456 0.76 -0.0 1,535,776 - DVU1456 0.77 -0.7 1,536,073 - +0.2 1,536,275 - -0.1 1,536,294 - -1.3 1,536,296 + +0.3 1,536,302 + -0.9 1,536,315 - -0.4 1,536,334 - -0.9 1,536,404 - trxB DVU1457 0.17 -0.2 1,536,415 - trxB DVU1457 0.18 -0.2 1,536,558 + trxB DVU1457 0.33 -1.0 1,536,602 - trxB DVU1457 0.38 -0.4 1,536,634 + trxB DVU1457 0.42 -1.7 1,536,691 + trxB DVU1457 0.48 -0.2 1,536,699 + trxB DVU1457 0.49 -0.6 1,536,699 - trxB DVU1457 0.49 -0.4 1,536,749 - trxB DVU1457 0.54 -0.4 1,536,776 + trxB DVU1457 0.57 -0.7 1,536,804 - trxB DVU1457 0.60 -1.5 1,536,805 + trxB DVU1457 0.60 -0.4 1,536,891 + trxB DVU1457 0.69 -0.4 1,536,965 - trxB DVU1457 0.77 -0.0
Or see this region's nucleotide sequence