Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05995 and BWI76_RS06000 are separated by 366 nucleotides BWI76_RS06000 and BWI76_RS06005 overlap by 4 nucleotides
BWI76_RS05995: BWI76_RS05995 - branched-chain amino acid ABC transporter substrate-binding protein, at 1,257,461 to 1,258,597
_RS05995
BWI76_RS06000: BWI76_RS06000 - ethanolamine ammonia-lyase small subunit, at 1,258,964 to 1,259,740
_RS06000
BWI76_RS06005: BWI76_RS06005 - ethanolamine ammonia lyase large subunit, at 1,259,737 to 1,261,125
_RS06005
Position (kb) 1258
1259
1260 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1258.192 kb on - strand, within BWI76_RS05995 at 1258.345 kb on - strand, within BWI76_RS05995 at 1258.384 kb on + strand, within BWI76_RS05995 at 1258.390 kb on + strand, within BWI76_RS05995 at 1258.420 kb on + strand, within BWI76_RS05995 at 1258.435 kb on - strand, within BWI76_RS05995 at 1258.439 kb on + strand, within BWI76_RS05995 at 1258.458 kb on + strand, within BWI76_RS05995 at 1258.467 kb on + strand, within BWI76_RS05995 at 1258.470 kb on - strand, within BWI76_RS05995 at 1258.470 kb on - strand, within BWI76_RS05995 at 1258.470 kb on - strand, within BWI76_RS05995 at 1258.477 kb on + strand, within BWI76_RS05995 at 1258.492 kb on - strand at 1258.504 kb on - strand at 1258.530 kb on - strand at 1258.533 kb on - strand at 1258.536 kb on + strand at 1258.536 kb on + strand at 1258.536 kb on + strand at 1258.536 kb on + strand at 1258.536 kb on + strand at 1258.544 kb on - strand at 1258.544 kb on - strand at 1258.544 kb on - strand at 1258.544 kb on - strand at 1258.544 kb on - strand at 1258.556 kb on - strand at 1258.603 kb on + strand at 1258.635 kb on + strand at 1258.635 kb on + strand at 1258.643 kb on + strand at 1258.684 kb on + strand at 1258.699 kb on + strand at 1258.702 kb on - strand at 1258.705 kb on - strand at 1258.740 kb on - strand at 1258.794 kb on + strand at 1258.794 kb on + strand at 1258.794 kb on + strand at 1258.794 kb on + strand at 1258.795 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.796 kb on + strand at 1258.802 kb on - strand at 1258.802 kb on - strand at 1258.803 kb on + strand at 1258.803 kb on + strand at 1258.803 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.804 kb on - strand at 1258.806 kb on + strand at 1258.806 kb on + strand at 1258.808 kb on + strand at 1258.808 kb on + strand at 1258.813 kb on + strand at 1258.814 kb on - strand at 1258.816 kb on - strand at 1258.816 kb on - strand at 1258.816 kb on - strand at 1258.816 kb on - strand at 1258.816 kb on - strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.818 kb on + strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.826 kb on - strand at 1258.829 kb on + strand at 1258.854 kb on + strand at 1258.854 kb on + strand at 1258.854 kb on + strand at 1258.854 kb on + strand at 1258.860 kb on + strand at 1258.861 kb on + strand at 1258.862 kb on - strand at 1258.872 kb on - strand at 1258.877 kb on - strand at 1258.886 kb on + strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.894 kb on - strand at 1258.905 kb on - strand at 1258.905 kb on - strand at 1258.925 kb on - strand at 1258.984 kb on - strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.002 kb on + strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.010 kb on - strand at 1259.022 kb on + strand at 1259.022 kb on + strand at 1259.035 kb on + strand at 1259.037 kb on + strand at 1259.040 kb on - strand at 1259.041 kb on - strand at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.043 kb on - strand, within BWI76_RS06000 at 1259.045 kb on - strand, within BWI76_RS06000 at 1259.057 kb on + strand, within BWI76_RS06000 at 1259.057 kb on + strand at 1259.057 kb on + strand, within BWI76_RS06000 at 1259.060 kb on - strand, within BWI76_RS06000 at 1259.065 kb on - strand, within BWI76_RS06000 at 1259.065 kb on - strand, within BWI76_RS06000 at 1259.070 kb on + strand, within BWI76_RS06000 at 1259.078 kb on - strand, within BWI76_RS06000 at 1259.078 kb on - strand, within BWI76_RS06000 at 1259.078 kb on - strand, within BWI76_RS06000 at 1259.078 kb on - strand at 1259.078 kb on - strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.080 kb on + strand, within BWI76_RS06000 at 1259.088 kb on - strand, within BWI76_RS06000 at 1259.088 kb on - strand, within BWI76_RS06000 at 1259.088 kb on - strand, within BWI76_RS06000 at 1259.088 kb on - strand, within BWI76_RS06000 at 1259.095 kb on + strand, within BWI76_RS06000 at 1259.103 kb on - strand, within BWI76_RS06000 at 1259.109 kb on - strand, within BWI76_RS06000 at 1259.110 kb on - strand, within BWI76_RS06000 at 1259.153 kb on - strand, within BWI76_RS06000 at 1259.160 kb on + strand, within BWI76_RS06000 at 1259.183 kb on - strand, within BWI76_RS06000 at 1259.183 kb on - strand, within BWI76_RS06000 at 1259.184 kb on + strand, within BWI76_RS06000 at 1259.185 kb on + strand, within BWI76_RS06000 at 1259.193 kb on - strand, within BWI76_RS06000 at 1259.193 kb on - strand, within BWI76_RS06000 at 1259.193 kb on - strand, within BWI76_RS06000 at 1259.193 kb on - strand, within BWI76_RS06000 at 1259.203 kb on - strand, within BWI76_RS06000 at 1259.207 kb on - strand, within BWI76_RS06000 at 1259.241 kb on + strand, within BWI76_RS06000 at 1259.249 kb on - strand, within BWI76_RS06000 at 1259.249 kb on - strand, within BWI76_RS06000 at 1259.249 kb on - strand, within BWI76_RS06000 at 1259.249 kb on - strand, within BWI76_RS06000 at 1259.313 kb on - strand, within BWI76_RS06000 at 1259.350 kb on - strand, within BWI76_RS06000 at 1259.375 kb on + strand, within BWI76_RS06000 at 1259.385 kb on + strand, within BWI76_RS06000 at 1259.442 kb on - strand, within BWI76_RS06000 at 1259.662 kb on + strand, within BWI76_RS06000 at 1259.743 kb on - strand at 1259.964 kb on + strand, within BWI76_RS06005 at 1259.972 kb on + strand, within BWI76_RS06005 at 1260.012 kb on - strand, within BWI76_RS06005 at 1260.047 kb on - strand, within BWI76_RS06005 at 1260.261 kb on - strand, within BWI76_RS06005 at 1260.468 kb on + strand, within BWI76_RS06005 at 1260.500 kb on - strand, within BWI76_RS06005 at 1260.517 kb on - strand, within BWI76_RS06005 at 1260.517 kb on - strand, within BWI76_RS06005 at 1260.557 kb on - strand, within BWI76_RS06005 at 1260.578 kb on + strand, within BWI76_RS06005 at 1260.662 kb on + strand, within BWI76_RS06005
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand remove 1,258,192 - BWI76_RS05995 0.64 +0.5 1,258,345 - BWI76_RS05995 0.78 +0.6 1,258,384 + BWI76_RS05995 0.81 +0.2 1,258,390 + BWI76_RS05995 0.82 -0.8 1,258,420 + BWI76_RS05995 0.84 +0.7 1,258,435 - BWI76_RS05995 0.86 -0.3 1,258,439 + BWI76_RS05995 0.86 -1.7 1,258,458 + BWI76_RS05995 0.88 -0.9 1,258,467 + BWI76_RS05995 0.88 -0.4 1,258,470 - BWI76_RS05995 0.89 -0.4 1,258,470 - BWI76_RS05995 0.89 -1.0 1,258,470 - BWI76_RS05995 0.89 -1.7 1,258,477 + BWI76_RS05995 0.89 -0.0 1,258,492 - +0.4 1,258,504 - -1.1 1,258,530 - +1.2 1,258,533 - -0.5 1,258,536 + +0.0 1,258,536 + +0.3 1,258,536 + -0.6 1,258,536 + +0.4 1,258,536 + +0.3 1,258,544 - +2.4 1,258,544 - +0.3 1,258,544 - +0.1 1,258,544 - +0.2 1,258,544 - +0.6 1,258,556 - -0.7 1,258,603 + +0.0 1,258,635 + -0.2 1,258,635 + -0.2 1,258,643 + -0.4 1,258,684 + -0.9 1,258,699 + +0.4 1,258,702 - -0.3 1,258,705 - -0.5 1,258,740 - -0.1 1,258,794 + +1.6 1,258,794 + -1.8 1,258,794 + +0.6 1,258,794 + +0.1 1,258,795 + -0.1 1,258,796 + +0.7 1,258,796 + -0.7 1,258,796 + +0.3 1,258,796 + -0.3 1,258,796 + -0.1 1,258,796 + -0.2 1,258,796 + +0.4 1,258,796 + -0.3 1,258,796 + +0.4 1,258,802 - +1.6 1,258,802 - +1.0 1,258,803 + -0.9 1,258,803 + +0.3 1,258,803 - -0.1 1,258,804 - +0.3 1,258,804 - +0.3 1,258,804 - +1.1 1,258,804 - -0.5 1,258,804 - -0.1 1,258,804 - +0.1 1,258,804 - +0.4 1,258,806 + +0.9 1,258,806 + -1.5 1,258,808 + -0.1 1,258,808 + -1.3 1,258,813 + -0.0 1,258,814 - +1.6 1,258,816 - -0.1 1,258,816 - +0.5 1,258,816 - -1.2 1,258,816 - -0.1 1,258,816 - +0.2 1,258,818 + -0.7 1,258,818 + -0.3 1,258,818 + -0.2 1,258,818 + +0.4 1,258,818 + +0.6 1,258,818 + -0.3 1,258,818 + +1.0 1,258,818 + +0.8 1,258,818 + +0.2 1,258,818 + +1.2 1,258,818 + -0.4 1,258,818 + +0.2 1,258,818 + +0.1 1,258,826 - -0.1 1,258,826 - -0.6 1,258,826 - -0.6 1,258,826 - +0.8 1,258,826 - -0.1 1,258,826 - -2.5 1,258,826 - -0.6 1,258,826 - +0.0 1,258,826 - +0.5 1,258,826 - -0.4 1,258,826 - -1.5 1,258,826 - +0.3 1,258,826 - -0.0 1,258,826 - +0.1 1,258,826 - +1.0 1,258,826 - +0.1 1,258,826 - +0.1 1,258,826 - +1.2 1,258,829 + -0.4 1,258,854 + +0.0 1,258,854 + -1.2 1,258,854 + -0.9 1,258,854 + -0.5 1,258,860 + -0.1 1,258,861 + -0.2 1,258,862 - +0.8 1,258,872 - -0.9 1,258,877 - +0.3 1,258,886 + -0.1 1,258,894 - +1.1 1,258,894 - +0.2 1,258,894 - -0.0 1,258,894 - +0.3 1,258,894 - -0.5 1,258,894 - +0.6 1,258,894 - -0.2 1,258,905 - -1.2 1,258,905 - -0.4 1,258,925 - -0.8 1,258,984 - -0.8 1,259,002 + +0.6 1,259,002 + -0.1 1,259,002 + +0.6 1,259,002 + +0.1 1,259,002 + +0.5 1,259,002 + +3.6 1,259,002 + -0.0 1,259,002 + +0.2 1,259,010 - +0.1 1,259,010 - -0.4 1,259,010 - +0.3 1,259,010 - -0.2 1,259,010 - -0.8 1,259,010 - -0.7 1,259,010 - +0.6 1,259,022 + -0.7 1,259,022 + +0.4 1,259,035 + -0.4 1,259,037 + +0.4 1,259,040 - -0.2 1,259,041 - +0.6 1,259,043 - BWI76_RS06000 0.10 +0.1 1,259,043 - BWI76_RS06000 0.10 +0.4 1,259,043 - BWI76_RS06000 0.10 +2.6 1,259,043 - BWI76_RS06000 0.10 +0.9 1,259,043 - BWI76_RS06000 0.10 +0.4 1,259,043 - BWI76_RS06000 0.10 -0.4 1,259,045 - BWI76_RS06000 0.10 +1.6 1,259,057 + BWI76_RS06000 0.12 +0.0 1,259,057 + +0.6 1,259,057 + BWI76_RS06000 0.12 +0.3 1,259,060 - BWI76_RS06000 0.12 -0.3 1,259,065 - BWI76_RS06000 0.13 +0.0 1,259,065 - BWI76_RS06000 0.13 +0.1 1,259,070 + BWI76_RS06000 0.14 +0.2 1,259,078 - BWI76_RS06000 0.15 +0.8 1,259,078 - BWI76_RS06000 0.15 -0.1 1,259,078 - BWI76_RS06000 0.15 +0.1 1,259,078 - -0.0 1,259,078 - BWI76_RS06000 0.15 +0.0 1,259,080 + BWI76_RS06000 0.15 -0.7 1,259,080 + BWI76_RS06000 0.15 -0.5 1,259,080 + BWI76_RS06000 0.15 +0.6 1,259,080 + BWI76_RS06000 0.15 +0.2 1,259,080 + BWI76_RS06000 0.15 -0.1 1,259,080 + BWI76_RS06000 0.15 -1.0 1,259,080 + BWI76_RS06000 0.15 +1.0 1,259,080 + BWI76_RS06000 0.15 +1.5 1,259,080 + BWI76_RS06000 0.15 -0.7 1,259,088 - BWI76_RS06000 0.16 +0.2 1,259,088 - BWI76_RS06000 0.16 +0.0 1,259,088 - BWI76_RS06000 0.16 +0.6 1,259,088 - BWI76_RS06000 0.16 +0.7 1,259,095 + BWI76_RS06000 0.17 +0.2 1,259,103 - BWI76_RS06000 0.18 +0.2 1,259,109 - BWI76_RS06000 0.19 -2.6 1,259,110 - BWI76_RS06000 0.19 -0.7 1,259,153 - BWI76_RS06000 0.24 -0.4 1,259,160 + BWI76_RS06000 0.25 +0.1 1,259,183 - BWI76_RS06000 0.28 +0.7 1,259,183 - BWI76_RS06000 0.28 +1.3 1,259,184 + BWI76_RS06000 0.28 -0.3 1,259,185 + BWI76_RS06000 0.28 +0.3 1,259,193 - BWI76_RS06000 0.29 +0.1 1,259,193 - BWI76_RS06000 0.29 -0.5 1,259,193 - BWI76_RS06000 0.29 -0.3 1,259,193 - BWI76_RS06000 0.29 +0.2 1,259,203 - BWI76_RS06000 0.31 +0.1 1,259,207 - BWI76_RS06000 0.31 +0.3 1,259,241 + BWI76_RS06000 0.36 +0.2 1,259,249 - BWI76_RS06000 0.37 -0.1 1,259,249 - BWI76_RS06000 0.37 -0.1 1,259,249 - BWI76_RS06000 0.37 -0.1 1,259,249 - BWI76_RS06000 0.37 -0.5 1,259,313 - BWI76_RS06000 0.45 -1.1 1,259,350 - BWI76_RS06000 0.50 -1.5 1,259,375 + BWI76_RS06000 0.53 +0.1 1,259,385 + BWI76_RS06000 0.54 -0.9 1,259,442 - BWI76_RS06000 0.62 +0.5 1,259,662 + BWI76_RS06000 0.90 +0.2 1,259,743 - +0.1 1,259,964 + BWI76_RS06005 0.16 +0.1 1,259,972 + BWI76_RS06005 0.17 -0.2 1,260,012 - BWI76_RS06005 0.20 +0.8 1,260,047 - BWI76_RS06005 0.22 +0.0 1,260,261 - BWI76_RS06005 0.38 -1.1 1,260,468 + BWI76_RS06005 0.53 +0.4 1,260,500 - BWI76_RS06005 0.55 +0.5 1,260,517 - BWI76_RS06005 0.56 +0.5 1,260,517 - BWI76_RS06005 0.56 -0.1 1,260,557 - BWI76_RS06005 0.59 +0.6 1,260,578 + BWI76_RS06005 0.61 -0.2 1,260,662 + BWI76_RS06005 0.67 +0.7
Or see this region's nucleotide sequence