Experiment: Varel_Bryant_medium_Glucose with Ciprofloxacin Hydrochloride 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0914 and BT0915 are separated by 313 nucleotides BT0915 and BT0916 are separated by 21 nucleotides BT0916 and BT0917 are separated by 122 nucleotides BT0917 and BT0918 are separated by 538 nucleotides
BT0914: BT0914 - recognition particle-docking protein FtsY (NCBI ptt file), at 1,125,323 to 1,126,282
BT0914
BT0915: BT0915 - 50S ribosomal protein L33 (NCBI ptt file), at 1,126,596 to 1,126,784
BT0915
BT0916: BT0916 - 50S ribosomal protein L28 (NCBI ptt file), at 1,126,806 to 1,127,066
BT0916
BT0917: BT0917 - competence-damage inducible protein (NCBI ptt file), at 1,127,189 to 1,128,349
BT0917
BT0918: BT0918 - integrase (NCBI ptt file), at 1,128,888 to 1,129,781
BT0918
Position (kb)
1127
1128
1129 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1126.299 kb on - strand at 1127.213 kb on + strand at 1127.224 kb on + strand at 1127.225 kb on - strand at 1127.277 kb on + strand at 1127.277 kb on + strand at 1127.508 kb on + strand, within BT0917 at 1127.509 kb on - strand, within BT0917 at 1127.572 kb on + strand, within BT0917 at 1127.674 kb on + strand, within BT0917 at 1127.701 kb on + strand, within BT0917 at 1127.873 kb on + strand, within BT0917 at 1127.921 kb on + strand, within BT0917 at 1127.990 kb on + strand, within BT0917 at 1128.171 kb on + strand, within BT0917 at 1128.322 kb on + strand at 1128.327 kb on - strand at 1128.403 kb on + strand at 1128.447 kb on + strand at 1128.453 kb on - strand at 1128.462 kb on - strand at 1128.464 kb on + strand at 1128.464 kb on + strand at 1128.469 kb on + strand at 1128.473 kb on + strand at 1128.473 kb on + strand at 1128.497 kb on + strand at 1128.517 kb on + strand at 1128.519 kb on + strand at 1128.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Ciprofloxacin Hydrochloride 0.05 mM remove 1,126,299 - -0.9 1,127,213 + -2.8 1,127,224 + -2.3 1,127,225 - -0.1 1,127,277 + -2.9 1,127,277 + -1.4 1,127,508 + BT0917 0.27 -3.6 1,127,509 - BT0917 0.28 -2.4 1,127,572 + BT0917 0.33 -2.3 1,127,674 + BT0917 0.42 -1.7 1,127,701 + BT0917 0.44 +0.1 1,127,873 + BT0917 0.59 +1.1 1,127,921 + BT0917 0.63 -1.5 1,127,990 + BT0917 0.69 +0.1 1,128,171 + BT0917 0.85 -3.1 1,128,322 + -0.9 1,128,327 - -0.8 1,128,403 + +1.1 1,128,447 + -0.6 1,128,453 - -2.3 1,128,462 - -0.5 1,128,464 + +0.1 1,128,464 + -2.8 1,128,469 + -1.5 1,128,473 + -1.6 1,128,473 + -0.9 1,128,497 + -1.3 1,128,517 + +0.1 1,128,519 + -2.1 1,128,631 - -1.9
Or see this region's nucleotide sequence