Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nifS and rplI are separated by 45 nucleotides rplI and Synpcc7942_2560 are separated by 287 nucleotides Synpcc7942_2560 and desC are separated by 109 nucleotides
Synpcc7942_2558: nifS - cysteine desulfurase NifS, at 2,636,518 to 2,637,684
nifS
Synpcc7942_2559: rplI - 50S ribosomal protein L9, at 2,637,730 to 2,638,188
rplI
Synpcc7942_2560: Synpcc7942_2560 - Acetyltransferase, GNAT family, at 2,638,476 to 2,639,036
_2560
Synpcc7942_2561: desC - Delta-9 acyl-phospholipid desaturase, at 2,639,146 to 2,639,982
desC
Position (kb)
2637
2638
2639 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2636.800 kb on + strand, within nifS at 2636.802 kb on - strand, within nifS at 2636.810 kb on - strand, within nifS at 2636.989 kb on - strand, within nifS at 2637.125 kb on + strand, within nifS at 2637.140 kb on - strand, within nifS at 2637.188 kb on - strand, within nifS at 2637.269 kb on + strand, within nifS at 2637.316 kb on - strand, within nifS at 2637.419 kb on + strand, within nifS at 2637.577 kb on + strand at 2637.639 kb on + strand at 2637.697 kb on - strand at 2637.699 kb on + strand at 2637.703 kb on + strand at 2637.713 kb on - strand at 2637.727 kb on + strand at 2637.784 kb on + strand, within rplI at 2637.792 kb on - strand, within rplI at 2637.833 kb on - strand, within rplI at 2637.924 kb on - strand, within rplI at 2638.050 kb on + strand, within rplI at 2638.058 kb on - strand, within rplI at 2638.069 kb on + strand, within rplI at 2638.076 kb on + strand, within rplI at 2638.077 kb on - strand, within rplI at 2638.077 kb on - strand, within rplI at 2638.083 kb on - strand, within rplI at 2638.115 kb on + strand, within rplI at 2638.152 kb on + strand at 2638.198 kb on + strand at 2638.217 kb on + strand at 2638.220 kb on - strand at 2638.235 kb on + strand at 2638.253 kb on - strand at 2638.253 kb on - strand at 2638.266 kb on + strand at 2638.353 kb on - strand at 2638.363 kb on + strand at 2638.366 kb on - strand at 2638.376 kb on - strand at 2638.381 kb on - strand at 2638.381 kb on - strand at 2638.433 kb on - strand at 2638.479 kb on - strand at 2638.480 kb on + strand at 2638.493 kb on - strand at 2638.504 kb on + strand at 2638.512 kb on - strand at 2638.623 kb on + strand, within Synpcc7942_2560 at 2638.646 kb on - strand, within Synpcc7942_2560 at 2638.668 kb on - strand, within Synpcc7942_2560 at 2638.680 kb on - strand, within Synpcc7942_2560 at 2638.750 kb on + strand, within Synpcc7942_2560 at 2638.787 kb on + strand, within Synpcc7942_2560 at 2638.792 kb on + strand, within Synpcc7942_2560 at 2638.822 kb on - strand, within Synpcc7942_2560 at 2638.825 kb on + strand, within Synpcc7942_2560 at 2638.833 kb on - strand, within Synpcc7942_2560 at 2638.833 kb on - strand, within Synpcc7942_2560 at 2638.853 kb on - strand, within Synpcc7942_2560 at 2638.858 kb on - strand, within Synpcc7942_2560 at 2638.900 kb on - strand, within Synpcc7942_2560 at 2638.939 kb on + strand, within Synpcc7942_2560 at 2638.939 kb on + strand, within Synpcc7942_2560 at 2638.939 kb on + strand, within Synpcc7942_2560 at 2638.942 kb on - strand, within Synpcc7942_2560 at 2638.942 kb on - strand, within Synpcc7942_2560 at 2638.942 kb on - strand, within Synpcc7942_2560 at 2638.944 kb on + strand, within Synpcc7942_2560 at 2638.949 kb on + strand, within Synpcc7942_2560 at 2638.949 kb on + strand, within Synpcc7942_2560 at 2638.949 kb on + strand, within Synpcc7942_2560 at 2638.949 kb on + strand, within Synpcc7942_2560 at 2638.957 kb on - strand, within Synpcc7942_2560 at 2638.957 kb on - strand, within Synpcc7942_2560 at 2638.957 kb on - strand, within Synpcc7942_2560 at 2638.985 kb on + strand at 2638.993 kb on - strand at 2639.029 kb on - strand at 2639.039 kb on - strand at 2639.039 kb on - strand at 2639.062 kb on - strand at 2639.108 kb on - strand at 2639.121 kb on + strand at 2639.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 2,636,800 + nifS Synpcc7942_2558 0.24 +2.6 2,636,802 - nifS Synpcc7942_2558 0.24 +1.6 2,636,810 - nifS Synpcc7942_2558 0.25 +1.6 2,636,989 - nifS Synpcc7942_2558 0.40 +1.0 2,637,125 + nifS Synpcc7942_2558 0.52 +0.6 2,637,140 - nifS Synpcc7942_2558 0.53 -0.4 2,637,188 - nifS Synpcc7942_2558 0.57 +0.3 2,637,269 + nifS Synpcc7942_2558 0.64 +0.0 2,637,316 - nifS Synpcc7942_2558 0.68 +0.6 2,637,419 + nifS Synpcc7942_2558 0.77 +1.6 2,637,577 + +2.6 2,637,639 + -1.5 2,637,697 - +1.6 2,637,699 + -1.6 2,637,703 + -1.6 2,637,713 - +0.8 2,637,727 + +1.8 2,637,784 + rplI Synpcc7942_2559 0.12 -0.4 2,637,792 - rplI Synpcc7942_2559 0.14 -0.5 2,637,833 - rplI Synpcc7942_2559 0.22 +3.4 2,637,924 - rplI Synpcc7942_2559 0.42 +2.2 2,638,050 + rplI Synpcc7942_2559 0.70 -0.4 2,638,058 - rplI Synpcc7942_2559 0.71 +1.8 2,638,069 + rplI Synpcc7942_2559 0.74 -0.7 2,638,076 + rplI Synpcc7942_2559 0.75 +1.6 2,638,077 - rplI Synpcc7942_2559 0.76 +2.6 2,638,077 - rplI Synpcc7942_2559 0.76 +0.7 2,638,083 - rplI Synpcc7942_2559 0.77 +1.3 2,638,115 + rplI Synpcc7942_2559 0.84 +2.0 2,638,152 + +1.6 2,638,198 + -1.1 2,638,217 + +0.0 2,638,220 - -1.0 2,638,235 + +1.2 2,638,253 - +2.2 2,638,253 - +1.9 2,638,266 + -0.2 2,638,353 - +0.0 2,638,363 + +0.3 2,638,366 - -2.2 2,638,376 - +1.4 2,638,381 - -1.9 2,638,381 - +1.2 2,638,433 - -2.6 2,638,479 - -0.7 2,638,480 + -1.7 2,638,493 - +1.6 2,638,504 + -2.2 2,638,512 - +1.8 2,638,623 + Synpcc7942_2560 0.26 +0.6 2,638,646 - Synpcc7942_2560 0.30 -2.7 2,638,668 - Synpcc7942_2560 0.34 -0.1 2,638,680 - Synpcc7942_2560 0.36 +0.0 2,638,750 + Synpcc7942_2560 0.49 -0.7 2,638,787 + Synpcc7942_2560 0.55 -1.4 2,638,792 + Synpcc7942_2560 0.56 +2.7 2,638,822 - Synpcc7942_2560 0.62 +0.6 2,638,825 + Synpcc7942_2560 0.62 -2.2 2,638,833 - Synpcc7942_2560 0.64 +1.6 2,638,833 - Synpcc7942_2560 0.64 +0.9 2,638,853 - Synpcc7942_2560 0.67 +0.6 2,638,858 - Synpcc7942_2560 0.68 +0.0 2,638,900 - Synpcc7942_2560 0.76 +1.6 2,638,939 + Synpcc7942_2560 0.83 -0.4 2,638,939 + Synpcc7942_2560 0.83 +0.0 2,638,939 + Synpcc7942_2560 0.83 -0.4 2,638,942 - Synpcc7942_2560 0.83 +2.9 2,638,942 - Synpcc7942_2560 0.83 +2.2 2,638,942 - Synpcc7942_2560 0.83 -0.1 2,638,944 + Synpcc7942_2560 0.83 -0.4 2,638,949 + Synpcc7942_2560 0.84 +0.0 2,638,949 + Synpcc7942_2560 0.84 +2.6 2,638,949 + Synpcc7942_2560 0.84 +0.9 2,638,949 + Synpcc7942_2560 0.84 +0.6 2,638,957 - Synpcc7942_2560 0.86 -0.7 2,638,957 - Synpcc7942_2560 0.86 +3.2 2,638,957 - Synpcc7942_2560 0.86 +0.6 2,638,985 + +1.4 2,638,993 - +2.9 2,639,029 - +2.6 2,639,039 - -0.1 2,639,039 - -1.6 2,639,062 - +1.6 2,639,108 - -1.4 2,639,121 + -0.7 2,639,129 - -1.1
Or see this region's nucleotide sequence