Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1903 and hlyD are separated by 15 nucleotides hlyD and hlyB are separated by 41 nucleotides
Synpcc7942_1903: Synpcc7942_1903 - hypothetical protein, at 1,976,983 to 1,978,113
_1903
Synpcc7942_1904: hlyD - hemolysin secretion protein-like, at 1,978,129 to 1,979,454
hlyD
Synpcc7942_1905: hlyB - cyclic nucleotide-binding domain (cNMP-BD) protein, at 1,979,496 to 1,982,498
hlyB
Position (kb)
1978
1979
1980 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1977.517 kb on + strand, within Synpcc7942_1903 at 1977.718 kb on - strand, within Synpcc7942_1903 at 1977.835 kb on - strand, within Synpcc7942_1903 at 1978.134 kb on + strand at 1978.366 kb on - strand, within hlyD at 1978.550 kb on - strand, within hlyD at 1978.572 kb on - strand, within hlyD at 1978.661 kb on - strand, within hlyD at 1978.738 kb on + strand, within hlyD at 1978.747 kb on - strand, within hlyD at 1978.792 kb on - strand, within hlyD at 1978.807 kb on + strand, within hlyD at 1978.832 kb on + strand, within hlyD at 1978.842 kb on - strand, within hlyD at 1978.938 kb on + strand, within hlyD at 1979.010 kb on - strand, within hlyD at 1979.096 kb on + strand, within hlyD at 1979.298 kb on - strand, within hlyD at 1979.300 kb on - strand, within hlyD at 1979.317 kb on + strand, within hlyD at 1979.487 kb on - strand at 1979.487 kb on - strand at 1979.487 kb on - strand at 1979.487 kb on - strand at 1979.494 kb on + strand at 1979.571 kb on - strand at 1979.595 kb on - strand at 1979.626 kb on + strand at 1979.626 kb on + strand at 1979.626 kb on + strand at 1979.626 kb on + strand at 1979.626 kb on + strand at 1979.634 kb on - strand at 1979.634 kb on - strand at 1979.634 kb on - strand at 1979.656 kb on + strand at 1979.669 kb on - strand at 1979.686 kb on + strand at 1979.762 kb on + strand at 1979.767 kb on + strand at 1979.767 kb on + strand at 1979.767 kb on + strand at 1979.775 kb on - strand at 1979.812 kb on + strand, within hlyB at 1979.833 kb on + strand, within hlyB at 1979.833 kb on + strand, within hlyB at 1979.841 kb on - strand, within hlyB at 1979.843 kb on - strand, within hlyB at 1979.970 kb on + strand at 1979.970 kb on + strand, within hlyB at 1980.101 kb on + strand, within hlyB at 1980.101 kb on + strand, within hlyB at 1980.101 kb on + strand, within hlyB at 1980.109 kb on - strand, within hlyB at 1980.109 kb on - strand, within hlyB at 1980.109 kb on - strand, within hlyB at 1980.111 kb on + strand, within hlyB at 1980.114 kb on + strand, within hlyB at 1980.350 kb on - strand, within hlyB at 1980.368 kb on + strand, within hlyB at 1980.402 kb on + strand, within hlyB at 1980.449 kb on + strand, within hlyB
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 1,977,517 + Synpcc7942_1903 0.47 +1.1 1,977,718 - Synpcc7942_1903 0.65 +2.0 1,977,835 - Synpcc7942_1903 0.75 +1.6 1,978,134 + -2.1 1,978,366 - hlyD Synpcc7942_1904 0.18 +0.1 1,978,550 - hlyD Synpcc7942_1904 0.32 +3.8 1,978,572 - hlyD Synpcc7942_1904 0.33 +1.2 1,978,661 - hlyD Synpcc7942_1904 0.40 -0.4 1,978,738 + hlyD Synpcc7942_1904 0.46 -2.1 1,978,747 - hlyD Synpcc7942_1904 0.47 -0.9 1,978,792 - hlyD Synpcc7942_1904 0.50 +1.4 1,978,807 + hlyD Synpcc7942_1904 0.51 -0.7 1,978,832 + hlyD Synpcc7942_1904 0.53 +0.3 1,978,842 - hlyD Synpcc7942_1904 0.54 +0.2 1,978,938 + hlyD Synpcc7942_1904 0.61 -2.5 1,979,010 - hlyD Synpcc7942_1904 0.66 +2.4 1,979,096 + hlyD Synpcc7942_1904 0.73 -0.4 1,979,298 - hlyD Synpcc7942_1904 0.88 +1.3 1,979,300 - hlyD Synpcc7942_1904 0.88 +1.4 1,979,317 + hlyD Synpcc7942_1904 0.90 -0.4 1,979,487 - -1.5 1,979,487 - +2.9 1,979,487 - -0.7 1,979,487 - -0.1 1,979,494 + +0.9 1,979,571 - +2.6 1,979,595 - +0.3 1,979,626 + +0.6 1,979,626 + +1.1 1,979,626 + +0.4 1,979,626 + +0.4 1,979,626 + +3.1 1,979,634 - -0.9 1,979,634 - -1.3 1,979,634 - +0.6 1,979,656 + +1.9 1,979,669 - -0.0 1,979,686 + +2.1 1,979,762 + -1.2 1,979,767 + +1.9 1,979,767 + -1.1 1,979,767 + +1.6 1,979,775 - -0.8 1,979,812 + hlyB Synpcc7942_1905 0.11 -2.4 1,979,833 + hlyB Synpcc7942_1905 0.11 -2.0 1,979,833 + hlyB Synpcc7942_1905 0.11 -0.5 1,979,841 - hlyB Synpcc7942_1905 0.11 -0.8 1,979,843 - hlyB Synpcc7942_1905 0.12 -2.8 1,979,970 + +0.1 1,979,970 + hlyB Synpcc7942_1905 0.16 -0.8 1,980,101 + hlyB Synpcc7942_1905 0.20 -0.3 1,980,101 + hlyB Synpcc7942_1905 0.20 -1.1 1,980,101 + hlyB Synpcc7942_1905 0.20 -0.3 1,980,109 - hlyB Synpcc7942_1905 0.20 +0.6 1,980,109 - hlyB Synpcc7942_1905 0.20 -0.3 1,980,109 - hlyB Synpcc7942_1905 0.20 -1.2 1,980,111 + hlyB Synpcc7942_1905 0.20 +0.1 1,980,114 + hlyB Synpcc7942_1905 0.21 +3.1 1,980,350 - hlyB Synpcc7942_1905 0.28 -2.5 1,980,368 + hlyB Synpcc7942_1905 0.29 -2.8 1,980,402 + hlyB Synpcc7942_1905 0.30 +0.1 1,980,449 + hlyB Synpcc7942_1905 0.32 +3.1
Or see this region's nucleotide sequence