Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt icd and Synpcc7942_1720 are separated by 155 nucleotides Synpcc7942_1720 and Synpcc7942_1721 are separated by 819 nucleotides
Synpcc7942_1719: icd - isocitrate dehydrogenase, at 1,790,369 to 1,791,796
icd
Synpcc7942_1720: Synpcc7942_1720 - hypothetical protein, at 1,791,952 to 1,792,392
_1720
Synpcc7942_1721: Synpcc7942_1721 - PBS lyase HEAT-like repeat, at 1,793,212 to 1,793,916
_1721
Position (kb)
1791
1792
1793 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1791.803 kb on + strand at 1791.836 kb on + strand at 1791.838 kb on + strand at 1791.844 kb on - strand at 1791.855 kb on + strand at 1791.856 kb on - strand at 1791.861 kb on - strand at 1791.905 kb on + strand at 1791.929 kb on - strand at 1791.934 kb on + strand at 1791.942 kb on + strand at 1791.942 kb on + strand at 1791.942 kb on + strand at 1791.952 kb on - strand at 1791.957 kb on - strand at 1791.977 kb on + strand at 1791.977 kb on + strand at 1792.050 kb on + strand, within Synpcc7942_1720 at 1792.058 kb on - strand, within Synpcc7942_1720 at 1792.058 kb on - strand, within Synpcc7942_1720 at 1792.071 kb on + strand, within Synpcc7942_1720 at 1792.071 kb on + strand, within Synpcc7942_1720 at 1792.079 kb on - strand, within Synpcc7942_1720 at 1792.079 kb on - strand, within Synpcc7942_1720 at 1792.079 kb on - strand at 1792.079 kb on - strand, within Synpcc7942_1720 at 1792.096 kb on - strand, within Synpcc7942_1720 at 1792.097 kb on - strand, within Synpcc7942_1720 at 1792.115 kb on + strand, within Synpcc7942_1720 at 1792.117 kb on - strand, within Synpcc7942_1720 at 1792.123 kb on - strand, within Synpcc7942_1720 at 1792.129 kb on + strand at 1792.150 kb on + strand, within Synpcc7942_1720 at 1792.195 kb on - strand at 1792.210 kb on + strand, within Synpcc7942_1720 at 1792.210 kb on + strand, within Synpcc7942_1720 at 1792.218 kb on - strand at 1792.218 kb on - strand, within Synpcc7942_1720 at 1792.218 kb on - strand, within Synpcc7942_1720 at 1792.220 kb on - strand, within Synpcc7942_1720 at 1792.307 kb on + strand, within Synpcc7942_1720 at 1792.309 kb on - strand at 1792.338 kb on - strand, within Synpcc7942_1720 at 1792.349 kb on + strand at 1792.359 kb on + strand at 1792.359 kb on + strand at 1792.359 kb on + strand at 1792.367 kb on - strand at 1792.367 kb on - strand at 1792.367 kb on - strand at 1792.367 kb on - strand at 1792.367 kb on - strand at 1792.395 kb on - strand at 1792.400 kb on - strand at 1792.430 kb on - strand at 1792.443 kb on - strand at 1792.450 kb on - strand at 1792.503 kb on - strand at 1792.551 kb on - strand at 1792.559 kb on + strand at 1792.560 kb on - strand at 1792.560 kb on - strand at 1792.561 kb on + strand at 1792.561 kb on + strand at 1792.564 kb on + strand at 1792.569 kb on - strand at 1792.569 kb on - strand at 1792.572 kb on - strand at 1792.572 kb on - strand at 1792.572 kb on - strand at 1792.626 kb on + strand at 1792.631 kb on + strand at 1792.767 kb on - strand at 1792.767 kb on - strand at 1792.768 kb on - strand at 1793.018 kb on - strand at 1793.078 kb on - strand at 1793.080 kb on + strand at 1793.091 kb on + strand at 1793.145 kb on + strand at 1793.239 kb on - strand at 1793.318 kb on + strand, within Synpcc7942_1721 at 1793.333 kb on + strand, within Synpcc7942_1721 at 1793.341 kb on - strand, within Synpcc7942_1721 at 1793.346 kb on + strand, within Synpcc7942_1721 at 1793.354 kb on + strand, within Synpcc7942_1721 at 1793.370 kb on + strand, within Synpcc7942_1721 at 1793.390 kb on + strand, within Synpcc7942_1721
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 1,791,803 + -1.4 1,791,836 + +1.3 1,791,838 + -0.3 1,791,844 - -0.3 1,791,855 + +0.4 1,791,856 - -1.3 1,791,861 - -1.2 1,791,905 + +2.6 1,791,929 - -1.6 1,791,934 + -0.7 1,791,942 + +1.0 1,791,942 + +1.6 1,791,942 + -1.4 1,791,952 - -2.0 1,791,957 - -1.7 1,791,977 + +0.2 1,791,977 + -1.3 1,792,050 + Synpcc7942_1720 0.22 -0.6 1,792,058 - Synpcc7942_1720 0.24 +0.3 1,792,058 - Synpcc7942_1720 0.24 -1.3 1,792,071 + Synpcc7942_1720 0.27 +2.6 1,792,071 + Synpcc7942_1720 0.27 -0.7 1,792,079 - Synpcc7942_1720 0.29 +1.6 1,792,079 - Synpcc7942_1720 0.29 +0.6 1,792,079 - -0.3 1,792,079 - Synpcc7942_1720 0.29 +1.6 1,792,096 - Synpcc7942_1720 0.33 +0.6 1,792,097 - Synpcc7942_1720 0.33 +2.0 1,792,115 + Synpcc7942_1720 0.37 -1.1 1,792,117 - Synpcc7942_1720 0.37 -0.0 1,792,123 - Synpcc7942_1720 0.39 -0.4 1,792,129 + -0.4 1,792,150 + Synpcc7942_1720 0.45 -1.5 1,792,195 - -0.7 1,792,210 + Synpcc7942_1720 0.59 -1.4 1,792,210 + Synpcc7942_1720 0.59 -1.5 1,792,218 - +0.3 1,792,218 - Synpcc7942_1720 0.60 -1.0 1,792,218 - Synpcc7942_1720 0.60 +1.6 1,792,220 - Synpcc7942_1720 0.61 -2.0 1,792,307 + Synpcc7942_1720 0.80 -2.0 1,792,309 - -0.2 1,792,338 - Synpcc7942_1720 0.88 -0.6 1,792,349 + +1.2 1,792,359 + -0.1 1,792,359 + +0.6 1,792,359 + +3.7 1,792,367 - -1.7 1,792,367 - -2.5 1,792,367 - -0.7 1,792,367 - +2.2 1,792,367 - -0.0 1,792,395 - +2.4 1,792,400 - +2.0 1,792,430 - -0.9 1,792,443 - -0.4 1,792,450 - -1.9 1,792,503 - +0.6 1,792,551 - -2.2 1,792,559 + -0.2 1,792,560 - -0.4 1,792,560 - -0.0 1,792,561 + +0.6 1,792,561 + +0.6 1,792,564 + +0.3 1,792,569 - -1.0 1,792,569 - +0.3 1,792,572 - -3.0 1,792,572 - +2.2 1,792,572 - -0.7 1,792,626 + -1.9 1,792,631 + -0.4 1,792,767 - +0.1 1,792,767 - +0.6 1,792,768 - -0.1 1,793,018 - -0.1 1,793,078 - +2.3 1,793,080 + -1.4 1,793,091 + +0.1 1,793,145 + -0.0 1,793,239 - +2.6 1,793,318 + Synpcc7942_1721 0.15 -0.0 1,793,333 + Synpcc7942_1721 0.17 +1.6 1,793,341 - Synpcc7942_1721 0.18 +2.6 1,793,346 + Synpcc7942_1721 0.19 -0.2 1,793,354 + Synpcc7942_1721 0.20 +2.6 1,793,370 + Synpcc7942_1721 0.22 -0.4 1,793,390 + Synpcc7942_1721 0.25 -2.0
Or see this region's nucleotide sequence