Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt stbB and Synpcc7942_1538 are separated by 90 nucleotides Synpcc7942_1538 and Synpcc7942_1539 are separated by 149 nucleotides Synpcc7942_1539 and isiC are separated by 37 nucleotides isiC and isiB are separated by 89 nucleotides isiB and isiA are separated by 184 nucleotides
Synpcc7942_1537: stbB - hypothetical protein, at 1,596,668 to 1,596,877
stbB
Synpcc7942_1538: Synpcc7942_1538 - hypothetical protein, at 1,596,968 to 1,597,366
_1538
Synpcc7942_1539: Synpcc7942_1539 - hypothetical protein, at 1,597,516 to 1,597,818
_1539
Synpcc7942_1540: isiC - hypothetical protein, at 1,597,856 to 1,598,566
isiC
Synpcc7942_1541: isiB - flavodoxin FldA, at 1,598,656 to 1,599,168
isiB
Synpcc7942_1542: isiA - iron-stress chlorophyll-binding protein, at 1,599,353 to 1,600,381
isiA
Position (kb)
1597
1598
1599 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1596.871 kb on + strand at 1596.879 kb on - strand at 1596.879 kb on - strand at 1596.879 kb on - strand at 1596.887 kb on - strand at 1596.897 kb on - strand at 1597.002 kb on - strand at 1597.016 kb on + strand, within Synpcc7942_1538 at 1597.086 kb on + strand, within Synpcc7942_1538 at 1597.225 kb on + strand, within Synpcc7942_1538 at 1597.225 kb on + strand, within Synpcc7942_1538 at 1597.233 kb on - strand, within Synpcc7942_1538 at 1597.276 kb on + strand, within Synpcc7942_1538 at 1597.389 kb on + strand at 1597.458 kb on + strand at 1597.466 kb on + strand at 1597.515 kb on + strand at 1597.515 kb on + strand at 1597.537 kb on - strand at 1597.539 kb on + strand at 1597.547 kb on - strand, within Synpcc7942_1539 at 1597.551 kb on + strand, within Synpcc7942_1539 at 1597.585 kb on - strand, within Synpcc7942_1539 at 1597.587 kb on + strand, within Synpcc7942_1539 at 1597.587 kb on + strand, within Synpcc7942_1539 at 1597.595 kb on - strand, within Synpcc7942_1539 at 1597.595 kb on - strand, within Synpcc7942_1539 at 1597.598 kb on + strand, within Synpcc7942_1539 at 1597.635 kb on + strand, within Synpcc7942_1539 at 1597.640 kb on + strand, within Synpcc7942_1539 at 1597.641 kb on - strand, within Synpcc7942_1539 at 1597.641 kb on - strand, within Synpcc7942_1539 at 1597.643 kb on + strand, within Synpcc7942_1539 at 1597.645 kb on + strand, within Synpcc7942_1539 at 1597.645 kb on + strand, within Synpcc7942_1539 at 1597.647 kb on + strand, within Synpcc7942_1539 at 1597.647 kb on + strand, within Synpcc7942_1539 at 1597.651 kb on - strand, within Synpcc7942_1539 at 1597.651 kb on - strand, within Synpcc7942_1539 at 1597.653 kb on - strand, within Synpcc7942_1539 at 1597.655 kb on - strand, within Synpcc7942_1539 at 1597.724 kb on - strand, within Synpcc7942_1539 at 1597.724 kb on - strand, within Synpcc7942_1539 at 1597.846 kb on + strand at 1597.846 kb on + strand at 1597.914 kb on + strand at 1598.003 kb on - strand, within isiC at 1598.003 kb on - strand, within isiC at 1598.322 kb on + strand, within isiC at 1598.358 kb on - strand, within isiC at 1598.408 kb on + strand, within isiC at 1598.418 kb on + strand, within isiC at 1598.446 kb on + strand, within isiC at 1598.513 kb on + strand at 1598.521 kb on - strand at 1598.535 kb on - strand at 1598.537 kb on + strand at 1598.545 kb on - strand at 1598.545 kb on - strand at 1598.555 kb on - strand at 1598.562 kb on + strand at 1598.608 kb on + strand at 1598.608 kb on + strand at 1598.616 kb on - strand at 1598.620 kb on + strand at 1598.620 kb on + strand at 1598.626 kb on - strand at 1598.654 kb on + strand at 1598.779 kb on - strand, within isiB at 1598.794 kb on + strand, within isiB at 1598.973 kb on - strand, within isiB at 1598.982 kb on - strand, within isiB at 1599.070 kb on - strand, within isiB at 1599.170 kb on + strand at 1599.215 kb on + strand at 1599.215 kb on + strand at 1599.215 kb on + strand at 1599.223 kb on - strand at 1599.223 kb on - strand at 1599.223 kb on - strand at 1599.359 kb on - strand at 1599.369 kb on - strand at 1599.369 kb on - strand at 1599.409 kb on + strand at 1599.451 kb on - strand at 1599.478 kb on - strand, within isiA at 1599.487 kb on + strand, within isiA at 1599.488 kb on - strand, within isiA at 1599.522 kb on + strand, within isiA at 1599.542 kb on - strand, within isiA
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 1,596,871 + -1.0 1,596,879 - -1.1 1,596,879 - -0.9 1,596,879 - +0.3 1,596,887 - -1.0 1,596,897 - -2.0 1,597,002 - +0.3 1,597,016 + Synpcc7942_1538 0.12 -1.2 1,597,086 + Synpcc7942_1538 0.30 -2.2 1,597,225 + Synpcc7942_1538 0.64 +1.1 1,597,225 + Synpcc7942_1538 0.64 +0.6 1,597,233 - Synpcc7942_1538 0.66 +0.0 1,597,276 + Synpcc7942_1538 0.77 +1.6 1,597,389 + -1.4 1,597,458 + +1.6 1,597,466 + +1.0 1,597,515 + +3.2 1,597,515 + -0.7 1,597,537 - -2.1 1,597,539 + +1.1 1,597,547 - Synpcc7942_1539 0.10 -1.2 1,597,551 + Synpcc7942_1539 0.12 +1.6 1,597,585 - Synpcc7942_1539 0.23 +0.3 1,597,587 + Synpcc7942_1539 0.23 +0.6 1,597,587 + Synpcc7942_1539 0.23 -1.5 1,597,595 - Synpcc7942_1539 0.26 -1.6 1,597,595 - Synpcc7942_1539 0.26 +0.0 1,597,598 + Synpcc7942_1539 0.27 +1.6 1,597,635 + Synpcc7942_1539 0.39 -1.9 1,597,640 + Synpcc7942_1539 0.41 +0.6 1,597,641 - Synpcc7942_1539 0.41 -3.0 1,597,641 - Synpcc7942_1539 0.41 +1.6 1,597,643 + Synpcc7942_1539 0.42 -1.7 1,597,645 + Synpcc7942_1539 0.43 -0.8 1,597,645 + Synpcc7942_1539 0.43 -0.0 1,597,647 + Synpcc7942_1539 0.43 +0.6 1,597,647 + Synpcc7942_1539 0.43 -0.7 1,597,651 - Synpcc7942_1539 0.45 -1.0 1,597,651 - Synpcc7942_1539 0.45 -1.5 1,597,653 - Synpcc7942_1539 0.45 -0.9 1,597,655 - Synpcc7942_1539 0.46 -1.0 1,597,724 - Synpcc7942_1539 0.69 -0.5 1,597,724 - Synpcc7942_1539 0.69 -1.3 1,597,846 + -2.4 1,597,846 + +0.6 1,597,914 + -0.9 1,598,003 - isiC Synpcc7942_1540 0.21 -2.2 1,598,003 - isiC Synpcc7942_1540 0.21 -1.2 1,598,322 + isiC Synpcc7942_1540 0.66 +0.6 1,598,358 - isiC Synpcc7942_1540 0.71 -1.8 1,598,408 + isiC Synpcc7942_1540 0.78 -0.1 1,598,418 + isiC Synpcc7942_1540 0.79 +0.7 1,598,446 + isiC Synpcc7942_1540 0.83 -1.5 1,598,513 + -0.7 1,598,521 - -0.8 1,598,535 - -0.5 1,598,537 + -2.2 1,598,545 - +1.3 1,598,545 - -1.8 1,598,555 - -1.0 1,598,562 + +1.6 1,598,608 + +0.3 1,598,608 + -1.0 1,598,616 - -0.1 1,598,620 + +0.6 1,598,620 + -1.7 1,598,626 - -1.2 1,598,654 + -1.0 1,598,779 - isiB Synpcc7942_1541 0.24 +0.6 1,598,794 + isiB Synpcc7942_1541 0.27 +0.5 1,598,973 - isiB Synpcc7942_1541 0.62 -0.4 1,598,982 - isiB Synpcc7942_1541 0.64 -1.8 1,599,070 - isiB Synpcc7942_1541 0.81 -1.0 1,599,170 + -0.9 1,599,215 + +2.2 1,599,215 + -2.3 1,599,215 + +0.6 1,599,223 - -1.6 1,599,223 - -1.6 1,599,223 - +0.0 1,599,359 - -1.9 1,599,369 - +2.2 1,599,369 - -0.2 1,599,409 + -0.3 1,599,451 - +1.6 1,599,478 - isiA Synpcc7942_1542 0.12 +0.0 1,599,487 + isiA Synpcc7942_1542 0.13 -1.1 1,599,488 - isiA Synpcc7942_1542 0.13 +0.1 1,599,522 + isiA Synpcc7942_1542 0.16 -2.7 1,599,542 - isiA Synpcc7942_1542 0.18 +3.2
Or see this region's nucleotide sequence