Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minD and minE are separated by 14 nucleotides minE and Synpcc7942_0898 overlap by 4 nucleotides Synpcc7942_0898 and tRNA-Thr are separated by 13 nucleotides tRNA-Thr and Synpcc7942_0899 are separated by 95 nucleotides Synpcc7942_0899 and gifA are separated by 144 nucleotides gifA and Synpcc7942_0901 are separated by 136 nucleotides
Synpcc7942_0896: minD - septum site-determining protein MinD, at 906,832 to 907,638
minD
Synpcc7942_0897: minE - cell division topological specificity factor MinE, at 907,653 to 907,928
minE
Synpcc7942_0898: Synpcc7942_0898 - ferredoxin like protein, at 907,925 to 908,317
_0898
Synpcc7942_R0041: tRNA-Thr - tRNA-Thr, at 908,331 to 908,402
tRNA-Thr
Synpcc7942_0899: Synpcc7942_0899 - hypothetical protein, at 908,498 to 909,295
_0899
Synpcc7942_0900: gifA - hypothetical protein, at 909,440 to 909,643
gifA
Synpcc7942_0901: Synpcc7942_0901 - Haloalkane dehalogenase, at 909,780 to 910,676
_0901
Position (kb) 908
909
910 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 907.532 kb on - strand, within minD at 907.547 kb on - strand, within minD at 907.555 kb on + strand, within minD at 907.579 kb on - strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.925 kb on - strand at 907.933 kb on + strand at 908.045 kb on + strand, within Synpcc7942_0898 at 908.045 kb on + strand, within Synpcc7942_0898 at 908.053 kb on - strand, within Synpcc7942_0898 at 908.059 kb on - strand, within Synpcc7942_0898 at 908.097 kb on + strand, within Synpcc7942_0898 at 908.114 kb on + strand, within Synpcc7942_0898 at 908.128 kb on + strand, within Synpcc7942_0898 at 908.134 kb on + strand, within Synpcc7942_0898 at 908.154 kb on + strand, within Synpcc7942_0898 at 908.154 kb on + strand, within Synpcc7942_0898 at 908.171 kb on - strand, within Synpcc7942_0898 at 908.179 kb on - strand, within Synpcc7942_0898 at 908.184 kb on - strand, within Synpcc7942_0898 at 908.211 kb on + strand, within Synpcc7942_0898 at 908.219 kb on - strand, within Synpcc7942_0898 at 908.243 kb on + strand, within Synpcc7942_0898 at 908.272 kb on - strand, within Synpcc7942_0898 at 908.311 kb on + strand at 908.415 kb on - strand at 908.457 kb on + strand at 908.503 kb on - strand at 908.505 kb on + strand at 908.508 kb on + strand at 908.521 kb on - strand at 908.531 kb on - strand at 908.533 kb on + strand at 908.533 kb on + strand at 908.544 kb on + strand at 908.547 kb on - strand at 908.549 kb on - strand at 908.563 kb on + strand at 908.575 kb on + strand at 908.591 kb on - strand, within Synpcc7942_0899 at 908.681 kb on + strand, within Synpcc7942_0899 at 908.681 kb on + strand, within Synpcc7942_0899 at 908.681 kb on + strand, within Synpcc7942_0899 at 908.696 kb on + strand, within Synpcc7942_0899 at 908.755 kb on + strand, within Synpcc7942_0899 at 908.758 kb on + strand, within Synpcc7942_0899 at 908.766 kb on - strand, within Synpcc7942_0899 at 908.846 kb on - strand, within Synpcc7942_0899 at 908.860 kb on + strand, within Synpcc7942_0899 at 908.865 kb on + strand, within Synpcc7942_0899 at 908.868 kb on - strand, within Synpcc7942_0899 at 908.876 kb on + strand, within Synpcc7942_0899 at 908.922 kb on + strand, within Synpcc7942_0899 at 908.922 kb on + strand, within Synpcc7942_0899 at 908.922 kb on + strand, within Synpcc7942_0899 at 908.938 kb on - strand, within Synpcc7942_0899 at 908.955 kb on - strand, within Synpcc7942_0899 at 909.117 kb on + strand, within Synpcc7942_0899 at 909.117 kb on + strand, within Synpcc7942_0899 at 909.120 kb on - strand, within Synpcc7942_0899 at 909.122 kb on + strand, within Synpcc7942_0899 at 909.122 kb on + strand, within Synpcc7942_0899 at 909.125 kb on - strand, within Synpcc7942_0899 at 909.125 kb on - strand, within Synpcc7942_0899 at 909.150 kb on + strand, within Synpcc7942_0899 at 909.165 kb on - strand, within Synpcc7942_0899 at 909.179 kb on - strand, within Synpcc7942_0899 at 909.186 kb on - strand, within Synpcc7942_0899 at 909.213 kb on + strand, within Synpcc7942_0899 at 909.229 kb on + strand at 909.254 kb on + strand at 909.254 kb on + strand at 909.254 kb on + strand at 909.269 kb on + strand at 909.403 kb on + strand at 909.411 kb on - strand at 909.419 kb on + strand at 909.439 kb on - strand at 909.443 kb on + strand at 909.451 kb on - strand at 909.461 kb on - strand, within gifA at 909.464 kb on + strand, within gifA at 909.464 kb on + strand, within gifA at 909.472 kb on - strand, within gifA at 909.500 kb on - strand, within gifA at 909.505 kb on - strand, within gifA at 909.505 kb on - strand, within gifA at 909.505 kb on - strand, within gifA at 909.520 kb on - strand, within gifA at 909.540 kb on + strand, within gifA at 909.614 kb on - strand, within gifA at 909.628 kb on + strand at 909.638 kb on + strand at 909.777 kb on + strand at 909.782 kb on + strand at 909.821 kb on + strand at 909.824 kb on - strand at 909.879 kb on + strand, within Synpcc7942_0901 at 909.883 kb on + strand, within Synpcc7942_0901 at 909.893 kb on + strand, within Synpcc7942_0901 at 909.914 kb on - strand, within Synpcc7942_0901 at 909.939 kb on + strand, within Synpcc7942_0901 at 909.947 kb on - strand, within Synpcc7942_0901 at 909.947 kb on - strand, within Synpcc7942_0901 at 909.953 kb on - strand, within Synpcc7942_0901 at 909.962 kb on + strand, within Synpcc7942_0901 at 909.992 kb on - strand, within Synpcc7942_0901 at 910.002 kb on + strand, within Synpcc7942_0901 at 910.012 kb on + strand, within Synpcc7942_0901 at 910.020 kb on - strand, within Synpcc7942_0901 at 910.023 kb on + strand, within Synpcc7942_0901 at 910.036 kb on + strand, within Synpcc7942_0901 at 910.036 kb on + strand, within Synpcc7942_0901 at 910.044 kb on - strand, within Synpcc7942_0901 at 910.044 kb on - strand, within Synpcc7942_0901 at 910.054 kb on - strand, within Synpcc7942_0901 at 910.063 kb on + strand, within Synpcc7942_0901 at 910.073 kb on - strand, within Synpcc7942_0901 at 910.099 kb on + strand, within Synpcc7942_0901 at 910.104 kb on + strand, within Synpcc7942_0901 at 910.112 kb on - strand, within Synpcc7942_0901 at 910.112 kb on - strand, within Synpcc7942_0901 at 910.118 kb on - strand, within Synpcc7942_0901 at 910.134 kb on + strand, within Synpcc7942_0901 at 910.134 kb on + strand, within Synpcc7942_0901 at 910.134 kb on + strand, within Synpcc7942_0901 at 910.140 kb on + strand, within Synpcc7942_0901 at 910.142 kb on - strand, within Synpcc7942_0901 at 910.142 kb on - strand, within Synpcc7942_0901 at 910.142 kb on - strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.171 kb on + strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.181 kb on - strand, within Synpcc7942_0901 at 910.183 kb on - strand, within Synpcc7942_0901 at 910.208 kb on + strand, within Synpcc7942_0901 at 910.208 kb on + strand, within Synpcc7942_0901 at 910.211 kb on - strand, within Synpcc7942_0901 at 910.211 kb on - strand, within Synpcc7942_0901 at 910.216 kb on - strand, within Synpcc7942_0901 at 910.216 kb on - strand, within Synpcc7942_0901 at 910.216 kb on - strand, within Synpcc7942_0901 at 910.219 kb on - strand, within Synpcc7942_0901 at 910.220 kb on + strand, within Synpcc7942_0901 at 910.228 kb on - strand, within Synpcc7942_0901 at 910.242 kb on + strand, within Synpcc7942_0901 at 910.268 kb on + strand, within Synpcc7942_0901
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 907,532 - minD Synpcc7942_0896 0.87 -2.1 907,547 - minD Synpcc7942_0896 0.89 -1.5 907,555 + minD Synpcc7942_0896 0.90 +3.2 907,579 - +2.6 907,582 + -0.7 907,582 + +0.7 907,582 + -0.5 907,582 + -0.5 907,582 + -2.0 907,582 + -1.8 907,582 + -1.0 907,590 - +1.6 907,590 - -2.4 907,590 - -1.1 907,590 - -0.6 907,925 - +1.6 907,933 + +1.6 908,045 + Synpcc7942_0898 0.31 -0.8 908,045 + Synpcc7942_0898 0.31 -1.0 908,053 - Synpcc7942_0898 0.33 -0.2 908,059 - Synpcc7942_0898 0.34 +0.6 908,097 + Synpcc7942_0898 0.44 +2.9 908,114 + Synpcc7942_0898 0.48 -0.3 908,128 + Synpcc7942_0898 0.52 -1.1 908,134 + Synpcc7942_0898 0.53 -1.0 908,154 + Synpcc7942_0898 0.58 +1.6 908,154 + Synpcc7942_0898 0.58 -0.4 908,171 - Synpcc7942_0898 0.63 -0.0 908,179 - Synpcc7942_0898 0.65 +0.6 908,184 - Synpcc7942_0898 0.66 +0.0 908,211 + Synpcc7942_0898 0.73 -0.4 908,219 - Synpcc7942_0898 0.75 -0.9 908,243 + Synpcc7942_0898 0.81 -2.3 908,272 - Synpcc7942_0898 0.88 +0.6 908,311 + +1.6 908,415 - -0.8 908,457 + -1.9 908,503 - +0.6 908,505 + -0.1 908,508 + +0.7 908,521 - -0.5 908,531 - -2.0 908,533 + +1.1 908,533 + +0.6 908,544 + -1.0 908,547 - -1.7 908,549 - -1.2 908,563 + -1.6 908,575 + +0.0 908,591 - Synpcc7942_0899 0.12 +0.3 908,681 + Synpcc7942_0899 0.23 -0.7 908,681 + Synpcc7942_0899 0.23 +0.2 908,681 + Synpcc7942_0899 0.23 -1.2 908,696 + Synpcc7942_0899 0.25 -2.2 908,755 + Synpcc7942_0899 0.32 -1.0 908,758 + Synpcc7942_0899 0.33 -0.9 908,766 - Synpcc7942_0899 0.34 +0.6 908,846 - Synpcc7942_0899 0.44 +1.6 908,860 + Synpcc7942_0899 0.45 -0.3 908,865 + Synpcc7942_0899 0.46 -0.2 908,868 - Synpcc7942_0899 0.46 -0.7 908,876 + Synpcc7942_0899 0.47 -1.2 908,922 + Synpcc7942_0899 0.53 -0.3 908,922 + Synpcc7942_0899 0.53 +1.0 908,922 + Synpcc7942_0899 0.53 +1.7 908,938 - Synpcc7942_0899 0.55 -1.8 908,955 - Synpcc7942_0899 0.57 +1.3 909,117 + Synpcc7942_0899 0.78 -1.0 909,117 + Synpcc7942_0899 0.78 +1.3 909,120 - Synpcc7942_0899 0.78 -0.9 909,122 + Synpcc7942_0899 0.78 +1.0 909,122 + Synpcc7942_0899 0.78 +0.5 909,125 - Synpcc7942_0899 0.79 -1.4 909,125 - Synpcc7942_0899 0.79 -3.5 909,150 + Synpcc7942_0899 0.82 -0.0 909,165 - Synpcc7942_0899 0.84 +0.6 909,179 - Synpcc7942_0899 0.85 +1.0 909,186 - Synpcc7942_0899 0.86 -0.2 909,213 + Synpcc7942_0899 0.90 -0.8 909,229 + +2.2 909,254 + -0.4 909,254 + +1.0 909,254 + -0.0 909,269 + -0.8 909,403 + +0.0 909,411 - -1.2 909,419 + +1.2 909,439 - -0.9 909,443 + -1.8 909,451 - -2.8 909,461 - gifA Synpcc7942_0900 0.10 +1.3 909,464 + gifA Synpcc7942_0900 0.12 +2.6 909,464 + gifA Synpcc7942_0900 0.12 +1.4 909,472 - gifA Synpcc7942_0900 0.16 +0.6 909,500 - gifA Synpcc7942_0900 0.29 -1.3 909,505 - gifA Synpcc7942_0900 0.32 +2.6 909,505 - gifA Synpcc7942_0900 0.32 -1.8 909,505 - gifA Synpcc7942_0900 0.32 +0.6 909,520 - gifA Synpcc7942_0900 0.39 +3.6 909,540 + gifA Synpcc7942_0900 0.49 +3.2 909,614 - gifA Synpcc7942_0900 0.85 +1.6 909,628 + +1.6 909,638 + -0.1 909,777 + -0.6 909,782 + +3.3 909,821 + +2.4 909,824 - -1.6 909,879 + Synpcc7942_0901 0.11 -0.4 909,883 + Synpcc7942_0901 0.11 +0.0 909,893 + Synpcc7942_0901 0.13 +0.0 909,914 - Synpcc7942_0901 0.15 +2.6 909,939 + Synpcc7942_0901 0.18 +2.6 909,947 - Synpcc7942_0901 0.19 -1.5 909,947 - Synpcc7942_0901 0.19 -0.5 909,953 - Synpcc7942_0901 0.19 +0.6 909,962 + Synpcc7942_0901 0.20 -1.8 909,992 - Synpcc7942_0901 0.24 -1.3 910,002 + Synpcc7942_0901 0.25 +1.6 910,012 + Synpcc7942_0901 0.26 -1.7 910,020 - Synpcc7942_0901 0.27 -0.4 910,023 + Synpcc7942_0901 0.27 +0.6 910,036 + Synpcc7942_0901 0.29 -1.6 910,036 + Synpcc7942_0901 0.29 -0.9 910,044 - Synpcc7942_0901 0.29 +2.4 910,044 - Synpcc7942_0901 0.29 +0.6 910,054 - Synpcc7942_0901 0.31 +0.6 910,063 + Synpcc7942_0901 0.32 -2.6 910,073 - Synpcc7942_0901 0.33 -1.5 910,099 + Synpcc7942_0901 0.36 -0.3 910,104 + Synpcc7942_0901 0.36 -1.0 910,112 - Synpcc7942_0901 0.37 +1.6 910,112 - Synpcc7942_0901 0.37 +0.6 910,118 - Synpcc7942_0901 0.38 +0.6 910,134 + Synpcc7942_0901 0.39 +1.6 910,134 + Synpcc7942_0901 0.39 -1.9 910,134 + Synpcc7942_0901 0.39 -1.0 910,140 + Synpcc7942_0901 0.40 +2.7 910,142 - Synpcc7942_0901 0.40 -0.5 910,142 - Synpcc7942_0901 0.40 +0.6 910,142 - Synpcc7942_0901 0.40 +0.0 910,163 + Synpcc7942_0901 0.43 +1.0 910,163 + Synpcc7942_0901 0.43 -2.2 910,163 + Synpcc7942_0901 0.43 +2.2 910,163 + Synpcc7942_0901 0.43 -1.2 910,163 + Synpcc7942_0901 0.43 +0.6 910,171 + Synpcc7942_0901 0.44 -1.7 910,171 - Synpcc7942_0901 0.44 +0.8 910,171 - Synpcc7942_0901 0.44 +2.4 910,171 - Synpcc7942_0901 0.44 +2.4 910,171 - Synpcc7942_0901 0.44 -0.8 910,171 - Synpcc7942_0901 0.44 -1.1 910,171 - Synpcc7942_0901 0.44 -1.8 910,181 - Synpcc7942_0901 0.45 -0.5 910,183 - Synpcc7942_0901 0.45 -0.3 910,208 + Synpcc7942_0901 0.48 -1.4 910,208 + Synpcc7942_0901 0.48 +0.3 910,211 - Synpcc7942_0901 0.48 -2.2 910,211 - Synpcc7942_0901 0.48 -3.7 910,216 - Synpcc7942_0901 0.49 +0.9 910,216 - Synpcc7942_0901 0.49 -1.2 910,216 - Synpcc7942_0901 0.49 -0.7 910,219 - Synpcc7942_0901 0.49 +1.6 910,220 + Synpcc7942_0901 0.49 +2.0 910,228 - Synpcc7942_0901 0.50 -0.3 910,242 + Synpcc7942_0901 0.52 -0.4 910,268 + Synpcc7942_0901 0.54 +0.0
Or see this region's nucleotide sequence