Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisS and Synpcc7942_0814 are separated by 32 nucleotides Synpcc7942_0814 and livF are separated by 6 nucleotides livF and Synpcc7942_0816 are separated by 84 nucleotides
Synpcc7942_0813: hisS - ATP phosphoribosyltransferase regulatory subunit, at 807,839 to 809,101
hisS
Synpcc7942_0814: Synpcc7942_0814 - ferredoxin-like protein, at 809,134 to 809,358
_0814
Synpcc7942_0815: livF - ATPase, at 809,365 to 810,096
livF
Synpcc7942_0816: Synpcc7942_0816 - diguanylate cyclase/phosphodiesterase, at 810,181 to 812,226
_0816
Position (kb) 809
810
811 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 809.058 kb on + strand at 809.059 kb on - strand at 809.083 kb on + strand at 809.123 kb on + strand at 809.353 kb on + strand at 809.360 kb on + strand at 809.372 kb on - strand at 809.382 kb on - strand at 809.477 kb on + strand, within livF at 809.502 kb on + strand, within livF at 809.547 kb on - strand, within livF at 809.567 kb on + strand, within livF at 809.572 kb on + strand, within livF at 809.575 kb on - strand, within livF at 809.588 kb on + strand, within livF at 809.607 kb on + strand, within livF at 809.659 kb on - strand, within livF at 809.675 kb on + strand, within livF at 809.729 kb on + strand, within livF at 809.729 kb on + strand, within livF at 809.729 kb on + strand, within livF at 809.729 kb on + strand, within livF at 809.729 kb on + strand, within livF at 809.729 kb on + strand, within livF at 809.729 kb on + strand, within livF at 809.737 kb on - strand, within livF at 809.737 kb on - strand, within livF at 809.774 kb on + strand, within livF at 809.779 kb on + strand, within livF at 809.805 kb on - strand, within livF at 809.864 kb on + strand, within livF at 809.893 kb on - strand, within livF at 809.925 kb on - strand, within livF at 809.925 kb on - strand, within livF at 809.952 kb on + strand, within livF at 809.969 kb on + strand, within livF at 809.973 kb on + strand, within livF at 810.032 kb on + strand at 810.040 kb on - strand at 810.071 kb on + strand at 810.074 kb on - strand at 810.077 kb on - strand at 810.092 kb on + strand at 810.097 kb on - strand at 810.224 kb on - strand at 810.240 kb on - strand at 810.309 kb on + strand at 810.409 kb on + strand, within Synpcc7942_0816 at 810.425 kb on + strand, within Synpcc7942_0816 at 810.463 kb on - strand, within Synpcc7942_0816 at 810.464 kb on - strand, within Synpcc7942_0816 at 810.583 kb on - strand, within Synpcc7942_0816 at 810.597 kb on - strand, within Synpcc7942_0816 at 810.613 kb on + strand, within Synpcc7942_0816 at 810.621 kb on - strand, within Synpcc7942_0816 at 810.661 kb on - strand, within Synpcc7942_0816 at 810.750 kb on - strand, within Synpcc7942_0816 at 810.885 kb on + strand, within Synpcc7942_0816 at 810.885 kb on + strand, within Synpcc7942_0816 at 810.886 kb on - strand, within Synpcc7942_0816 at 810.886 kb on - strand, within Synpcc7942_0816 at 810.893 kb on - strand, within Synpcc7942_0816 at 810.895 kb on + strand, within Synpcc7942_0816 at 810.937 kb on + strand, within Synpcc7942_0816 at 810.949 kb on + strand, within Synpcc7942_0816 at 810.949 kb on + strand, within Synpcc7942_0816 at 810.957 kb on - strand, within Synpcc7942_0816 at 810.957 kb on - strand, within Synpcc7942_0816 at 810.957 kb on - strand, within Synpcc7942_0816 at 810.979 kb on + strand, within Synpcc7942_0816 at 810.979 kb on + strand, within Synpcc7942_0816 at 810.999 kb on + strand, within Synpcc7942_0816 at 811.004 kb on + strand, within Synpcc7942_0816 at 811.007 kb on - strand, within Synpcc7942_0816 at 811.012 kb on - strand, within Synpcc7942_0816 at 811.012 kb on - strand, within Synpcc7942_0816 at 811.016 kb on + strand, within Synpcc7942_0816 at 811.085 kb on - strand, within Synpcc7942_0816 at 811.087 kb on + strand, within Synpcc7942_0816 at 811.095 kb on - strand, within Synpcc7942_0816 at 811.095 kb on - strand, within Synpcc7942_0816 at 811.095 kb on - strand, within Synpcc7942_0816
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 809,058 + +0.5 809,059 - +0.6 809,083 + +0.6 809,123 + +0.6 809,353 + -1.2 809,360 + -2.4 809,372 - -2.0 809,382 - -1.0 809,477 + livF Synpcc7942_0815 0.15 -1.8 809,502 + livF Synpcc7942_0815 0.19 +0.0 809,547 - livF Synpcc7942_0815 0.25 +1.6 809,567 + livF Synpcc7942_0815 0.28 +1.6 809,572 + livF Synpcc7942_0815 0.28 +0.7 809,575 - livF Synpcc7942_0815 0.29 +1.7 809,588 + livF Synpcc7942_0815 0.30 -0.2 809,607 + livF Synpcc7942_0815 0.33 -0.2 809,659 - livF Synpcc7942_0815 0.40 -1.2 809,675 + livF Synpcc7942_0815 0.42 -0.4 809,729 + livF Synpcc7942_0815 0.50 +1.6 809,729 + livF Synpcc7942_0815 0.50 +3.2 809,729 + livF Synpcc7942_0815 0.50 +1.6 809,729 + livF Synpcc7942_0815 0.50 -1.0 809,729 + livF Synpcc7942_0815 0.50 -0.1 809,729 + livF Synpcc7942_0815 0.50 -0.4 809,729 + livF Synpcc7942_0815 0.50 +1.9 809,737 - livF Synpcc7942_0815 0.51 +0.4 809,737 - livF Synpcc7942_0815 0.51 -1.3 809,774 + livF Synpcc7942_0815 0.56 +2.3 809,779 + livF Synpcc7942_0815 0.57 -0.9 809,805 - livF Synpcc7942_0815 0.60 -1.9 809,864 + livF Synpcc7942_0815 0.68 -0.5 809,893 - livF Synpcc7942_0815 0.72 +0.0 809,925 - livF Synpcc7942_0815 0.77 -2.2 809,925 - livF Synpcc7942_0815 0.77 -1.2 809,952 + livF Synpcc7942_0815 0.80 -1.6 809,969 + livF Synpcc7942_0815 0.83 -0.4 809,973 + livF Synpcc7942_0815 0.83 +0.6 810,032 + -0.7 810,040 - +1.6 810,071 + -0.6 810,074 - -1.6 810,077 - +0.1 810,092 + +1.2 810,097 - +1.1 810,224 - +2.2 810,240 - +1.9 810,309 + +0.9 810,409 + Synpcc7942_0816 0.11 +2.0 810,425 + Synpcc7942_0816 0.12 -1.4 810,463 - Synpcc7942_0816 0.14 +0.6 810,464 - Synpcc7942_0816 0.14 +1.6 810,583 - Synpcc7942_0816 0.20 -0.8 810,597 - Synpcc7942_0816 0.20 -0.9 810,613 + Synpcc7942_0816 0.21 -0.2 810,621 - Synpcc7942_0816 0.22 +2.2 810,661 - Synpcc7942_0816 0.23 -0.7 810,750 - Synpcc7942_0816 0.28 +0.4 810,885 + Synpcc7942_0816 0.34 -0.9 810,885 + Synpcc7942_0816 0.34 +1.6 810,886 - Synpcc7942_0816 0.34 -2.8 810,886 - Synpcc7942_0816 0.34 +0.9 810,893 - Synpcc7942_0816 0.35 -1.3 810,895 + Synpcc7942_0816 0.35 -1.0 810,937 + Synpcc7942_0816 0.37 +0.2 810,949 + Synpcc7942_0816 0.38 +3.2 810,949 + Synpcc7942_0816 0.38 +0.0 810,957 - Synpcc7942_0816 0.38 -0.8 810,957 - Synpcc7942_0816 0.38 +1.6 810,957 - Synpcc7942_0816 0.38 +1.3 810,979 + Synpcc7942_0816 0.39 -1.2 810,979 + Synpcc7942_0816 0.39 -0.4 810,999 + Synpcc7942_0816 0.40 +0.8 811,004 + Synpcc7942_0816 0.40 -1.0 811,007 - Synpcc7942_0816 0.40 -2.0 811,012 - Synpcc7942_0816 0.41 +1.6 811,012 - Synpcc7942_0816 0.41 -0.6 811,016 + Synpcc7942_0816 0.41 +0.6 811,085 - Synpcc7942_0816 0.44 -1.5 811,087 + Synpcc7942_0816 0.44 +1.6 811,095 - Synpcc7942_0816 0.45 +2.8 811,095 - Synpcc7942_0816 0.45 -1.4 811,095 - Synpcc7942_0816 0.45 +3.3
Or see this region's nucleotide sequence