Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt smf and act are separated by 32 nucleotides act and Synpcc7942_0705 overlap by 14 nucleotides Synpcc7942_0705 and cobL are separated by 6 nucleotides cobL and cad overlap by 20 nucleotides
Synpcc7942_0703: smf - DNA processing protein DprA, putative, at 694,072 to 695,280
smf
Synpcc7942_0704: act - Chloramphenicol O-acetyltransferase, at 695,313 to 695,948
act
Synpcc7942_0705: Synpcc7942_0705 - hypothetical protein, at 695,935 to 696,570
_0705
Synpcc7942_0706: cobL - precorrin-6B methylase, at 696,577 to 697,191
cobL
Synpcc7942_0707: cad - arginine decarboxylase, at 697,172 to 698,641
cad
Position (kb) 695
696
697 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 694.938 kb on + strand, within smf at 694.938 kb on + strand, within smf at 694.938 kb on + strand at 694.938 kb on + strand, within smf at 694.946 kb on - strand, within smf at 695.104 kb on + strand, within smf at 695.122 kb on + strand, within smf at 695.127 kb on + strand, within smf at 695.135 kb on - strand, within smf at 695.135 kb on - strand, within smf at 695.137 kb on - strand, within smf at 695.148 kb on - strand, within smf at 695.269 kb on - strand at 695.411 kb on + strand, within act at 695.556 kb on + strand, within act at 695.835 kb on + strand, within act at 696.145 kb on + strand, within Synpcc7942_0705 at 696.164 kb on + strand, within Synpcc7942_0705 at 696.209 kb on - strand, within Synpcc7942_0705 at 696.213 kb on + strand, within Synpcc7942_0705 at 696.301 kb on - strand, within Synpcc7942_0705 at 696.371 kb on - strand, within Synpcc7942_0705 at 696.387 kb on + strand, within Synpcc7942_0705 at 696.397 kb on + strand, within Synpcc7942_0705 at 696.397 kb on + strand, within Synpcc7942_0705 at 696.403 kb on - strand, within Synpcc7942_0705 at 696.464 kb on + strand, within Synpcc7942_0705 at 696.464 kb on - strand, within Synpcc7942_0705 at 696.466 kb on + strand, within Synpcc7942_0705 at 696.474 kb on - strand, within Synpcc7942_0705 at 696.474 kb on - strand at 696.474 kb on - strand, within Synpcc7942_0705 at 696.719 kb on + strand, within cobL at 696.722 kb on + strand, within cobL at 696.722 kb on + strand, within cobL at 696.724 kb on + strand, within cobL at 696.748 kb on + strand, within cobL at 696.756 kb on - strand, within cobL at 696.756 kb on - strand, within cobL at 696.770 kb on + strand, within cobL at 696.799 kb on - strand, within cobL at 696.799 kb on - strand, within cobL at 696.837 kb on + strand, within cobL at 696.845 kb on - strand, within cobL at 696.866 kb on - strand, within cobL at 696.888 kb on - strand, within cobL at 696.896 kb on + strand, within cobL at 696.923 kb on - strand, within cobL at 696.926 kb on + strand, within cobL at 696.930 kb on + strand, within cobL at 696.955 kb on - strand, within cobL at 696.983 kb on + strand, within cobL at 696.983 kb on + strand, within cobL at 697.040 kb on + strand, within cobL at 697.040 kb on - strand, within cobL at 697.055 kb on - strand, within cobL at 697.135 kb on - strand at 697.144 kb on + strand at 697.154 kb on + strand at 697.159 kb on + strand at 697.162 kb on - strand at 697.162 kb on - strand at 697.178 kb on - strand at 697.180 kb on + strand at 697.180 kb on + strand at 697.183 kb on - strand at 697.187 kb on + strand at 697.188 kb on - strand at 697.198 kb on + strand at 697.205 kb on + strand at 697.205 kb on + strand at 697.213 kb on - strand at 697.226 kb on + strand at 697.349 kb on + strand at 697.395 kb on - strand, within cad at 697.432 kb on + strand, within cad at 697.500 kb on - strand, within cad at 697.502 kb on - strand, within cad at 697.517 kb on + strand, within cad at 697.519 kb on - strand, within cad at 697.529 kb on - strand, within cad
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 694,938 + smf Synpcc7942_0703 0.72 -0.9 694,938 + smf Synpcc7942_0703 0.72 +1.6 694,938 + -1.4 694,938 + smf Synpcc7942_0703 0.72 -1.2 694,946 - smf Synpcc7942_0703 0.72 -0.1 695,104 + smf Synpcc7942_0703 0.85 -1.8 695,122 + smf Synpcc7942_0703 0.87 -1.6 695,127 + smf Synpcc7942_0703 0.87 -0.1 695,135 - smf Synpcc7942_0703 0.88 +0.6 695,135 - smf Synpcc7942_0703 0.88 -0.9 695,137 - smf Synpcc7942_0703 0.88 +1.6 695,148 - smf Synpcc7942_0703 0.89 +0.1 695,269 - +1.4 695,411 + act Synpcc7942_0704 0.15 +1.9 695,556 + act Synpcc7942_0704 0.38 +1.4 695,835 + act Synpcc7942_0704 0.82 -1.4 696,145 + Synpcc7942_0705 0.33 -1.2 696,164 + Synpcc7942_0705 0.36 +2.2 696,209 - Synpcc7942_0705 0.43 +1.6 696,213 + Synpcc7942_0705 0.44 -1.5 696,301 - Synpcc7942_0705 0.58 -1.6 696,371 - Synpcc7942_0705 0.69 -1.8 696,387 + Synpcc7942_0705 0.71 -2.4 696,397 + Synpcc7942_0705 0.73 +3.8 696,397 + Synpcc7942_0705 0.73 -2.1 696,403 - Synpcc7942_0705 0.74 +0.6 696,464 + Synpcc7942_0705 0.83 +0.9 696,464 - Synpcc7942_0705 0.83 +2.6 696,466 + Synpcc7942_0705 0.83 +2.6 696,474 - Synpcc7942_0705 0.85 -1.3 696,474 - +2.6 696,474 - Synpcc7942_0705 0.85 -1.0 696,719 + cobL Synpcc7942_0706 0.23 +1.6 696,722 + cobL Synpcc7942_0706 0.24 +0.0 696,722 + cobL Synpcc7942_0706 0.24 -2.6 696,724 + cobL Synpcc7942_0706 0.24 +1.6 696,748 + cobL Synpcc7942_0706 0.28 +1.6 696,756 - cobL Synpcc7942_0706 0.29 -0.7 696,756 - cobL Synpcc7942_0706 0.29 +0.0 696,770 + cobL Synpcc7942_0706 0.31 +0.0 696,799 - cobL Synpcc7942_0706 0.36 -0.4 696,799 - cobL Synpcc7942_0706 0.36 -2.0 696,837 + cobL Synpcc7942_0706 0.42 +0.0 696,845 - cobL Synpcc7942_0706 0.44 +2.6 696,866 - cobL Synpcc7942_0706 0.47 -2.2 696,888 - cobL Synpcc7942_0706 0.51 +1.6 696,896 + cobL Synpcc7942_0706 0.52 -1.2 696,923 - cobL Synpcc7942_0706 0.56 -2.1 696,926 + cobL Synpcc7942_0706 0.57 +2.6 696,930 + cobL Synpcc7942_0706 0.57 +1.2 696,955 - cobL Synpcc7942_0706 0.61 -1.4 696,983 + cobL Synpcc7942_0706 0.66 -0.7 696,983 + cobL Synpcc7942_0706 0.66 -0.2 697,040 + cobL Synpcc7942_0706 0.75 +0.3 697,040 - cobL Synpcc7942_0706 0.75 +1.6 697,055 - cobL Synpcc7942_0706 0.78 -2.2 697,135 - +0.6 697,144 + +0.6 697,154 + -0.4 697,159 + +1.6 697,162 - +1.6 697,162 - -2.4 697,178 - +0.0 697,180 + -1.1 697,180 + -2.0 697,183 - +0.9 697,187 + -1.3 697,188 - -0.7 697,198 + -1.1 697,205 + -1.8 697,205 + +2.6 697,213 - +1.6 697,226 + +1.6 697,349 + -1.0 697,395 - cad Synpcc7942_0707 0.15 +1.6 697,432 + cad Synpcc7942_0707 0.18 +1.6 697,500 - cad Synpcc7942_0707 0.22 -2.1 697,502 - cad Synpcc7942_0707 0.22 +0.0 697,517 + cad Synpcc7942_0707 0.23 +1.6 697,519 - cad Synpcc7942_0707 0.24 +1.6 697,529 - cad Synpcc7942_0707 0.24 -1.6
Or see this region's nucleotide sequence