Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0666 and Synpcc7942_0667 are separated by 223 nucleotides Synpcc7942_0667 and Synpcc7942_0668 are separated by 9 nucleotides Synpcc7942_0668 and mpg are separated by 93 nucleotides mpg and pyrB overlap by 4 nucleotides
Synpcc7942_0666: Synpcc7942_0666 - Heat shock protein DnaJ-like, at 663,141 to 663,761
_0666
Synpcc7942_0667: Synpcc7942_0667 - hypothetical protein, at 663,985 to 664,296
_0667
Synpcc7942_0668: Synpcc7942_0668 - hypothetical protein, at 664,306 to 664,446
_0668
Synpcc7942_0669: mpg - DNA-3-methyladenine glycosylase, at 664,540 to 665,124
mpg
Synpcc7942_0670: pyrB - aspartate carbamoyltransferase catalytic subunit, at 665,121 to 666,137
pyrB
Position (kb) 664
665 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 663.310 kb on - strand, within Synpcc7942_0666 at 663.332 kb on + strand, within Synpcc7942_0666 at 663.382 kb on + strand, within Synpcc7942_0666 at 663.389 kb on + strand, within Synpcc7942_0666 at 663.389 kb on - strand, within Synpcc7942_0666 at 663.390 kb on - strand, within Synpcc7942_0666 at 663.390 kb on - strand, within Synpcc7942_0666 at 663.419 kb on - strand, within Synpcc7942_0666 at 663.468 kb on - strand, within Synpcc7942_0666 at 663.501 kb on - strand, within Synpcc7942_0666 at 663.524 kb on + strand, within Synpcc7942_0666 at 663.533 kb on - strand, within Synpcc7942_0666 at 663.576 kb on - strand, within Synpcc7942_0666 at 663.577 kb on + strand, within Synpcc7942_0666 at 663.580 kb on + strand, within Synpcc7942_0666 at 663.598 kb on + strand, within Synpcc7942_0666 at 663.599 kb on - strand, within Synpcc7942_0666 at 663.613 kb on + strand, within Synpcc7942_0666 at 663.621 kb on - strand, within Synpcc7942_0666 at 663.685 kb on + strand, within Synpcc7942_0666 at 663.688 kb on - strand at 663.697 kb on + strand, within Synpcc7942_0666 at 663.700 kb on - strand at 663.763 kb on - strand at 663.802 kb on + strand at 663.810 kb on - strand at 663.810 kb on - strand at 663.812 kb on - strand at 663.817 kb on + strand at 663.817 kb on + strand at 663.827 kb on + strand at 663.835 kb on - strand at 663.857 kb on - strand at 663.857 kb on - strand at 663.860 kb on - strand at 663.882 kb on - strand at 663.882 kb on - strand at 663.897 kb on + strand at 663.897 kb on + strand at 663.933 kb on + strand at 663.941 kb on - strand at 663.969 kb on + strand at 663.977 kb on - strand at 664.011 kb on + strand at 664.038 kb on - strand, within Synpcc7942_0667 at 664.046 kb on - strand, within Synpcc7942_0667 at 664.061 kb on + strand, within Synpcc7942_0667 at 664.100 kb on + strand, within Synpcc7942_0667 at 664.172 kb on + strand, within Synpcc7942_0667 at 664.258 kb on + strand, within Synpcc7942_0667 at 664.306 kb on + strand at 664.384 kb on + strand, within Synpcc7942_0668 at 664.384 kb on + strand, within Synpcc7942_0668 at 664.384 kb on + strand, within Synpcc7942_0668 at 664.384 kb on + strand, within Synpcc7942_0668 at 664.392 kb on - strand, within Synpcc7942_0668 at 664.393 kb on - strand, within Synpcc7942_0668 at 664.454 kb on + strand at 664.454 kb on + strand at 664.454 kb on + strand at 664.462 kb on - strand at 664.473 kb on + strand at 664.478 kb on + strand at 664.577 kb on + strand at 664.600 kb on + strand, within mpg at 664.618 kb on - strand, within mpg at 664.636 kb on + strand, within mpg at 664.660 kb on + strand, within mpg at 664.695 kb on - strand at 664.697 kb on + strand, within mpg at 664.697 kb on + strand, within mpg at 664.699 kb on + strand, within mpg at 664.705 kb on - strand, within mpg at 664.720 kb on + strand, within mpg at 664.745 kb on + strand, within mpg at 664.755 kb on + strand, within mpg at 664.755 kb on + strand, within mpg at 664.755 kb on + strand, within mpg at 664.755 kb on + strand, within mpg at 664.763 kb on - strand, within mpg at 664.763 kb on - strand, within mpg at 664.763 kb on - strand, within mpg at 664.763 kb on - strand, within mpg at 664.780 kb on + strand, within mpg at 664.788 kb on - strand, within mpg at 664.790 kb on + strand at 664.824 kb on - strand, within mpg at 664.824 kb on - strand, within mpg at 664.891 kb on - strand, within mpg at 664.891 kb on - strand, within mpg at 664.891 kb on - strand, within mpg at 664.931 kb on + strand, within mpg at 664.931 kb on + strand, within mpg at 664.934 kb on - strand, within mpg at 664.947 kb on + strand, within mpg at 664.947 kb on + strand, within mpg at 664.947 kb on + strand, within mpg at 665.014 kb on + strand, within mpg at 665.014 kb on + strand, within mpg at 665.105 kb on + strand at 665.125 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 663,310 - Synpcc7942_0666 0.27 -1.9 663,332 + Synpcc7942_0666 0.31 +0.9 663,382 + Synpcc7942_0666 0.39 -2.2 663,389 + Synpcc7942_0666 0.40 +2.6 663,389 - Synpcc7942_0666 0.40 -1.2 663,390 - Synpcc7942_0666 0.40 -0.8 663,390 - Synpcc7942_0666 0.40 -1.1 663,419 - Synpcc7942_0666 0.45 -2.7 663,468 - Synpcc7942_0666 0.53 -0.7 663,501 - Synpcc7942_0666 0.58 +1.6 663,524 + Synpcc7942_0666 0.62 -1.3 663,533 - Synpcc7942_0666 0.63 +2.0 663,576 - Synpcc7942_0666 0.70 -2.7 663,577 + Synpcc7942_0666 0.70 -1.1 663,580 + Synpcc7942_0666 0.71 -1.1 663,598 + Synpcc7942_0666 0.74 +1.0 663,599 - Synpcc7942_0666 0.74 -0.7 663,613 + Synpcc7942_0666 0.76 +0.6 663,621 - Synpcc7942_0666 0.77 -0.7 663,685 + Synpcc7942_0666 0.88 -2.7 663,688 - -1.2 663,697 + Synpcc7942_0666 0.90 +0.6 663,700 - +1.6 663,763 - -2.2 663,802 + -2.1 663,810 - -2.9 663,810 - -1.1 663,812 - -0.1 663,817 + +2.0 663,817 + -2.3 663,827 + +2.2 663,835 - +1.4 663,857 - -0.7 663,857 - +2.6 663,860 - +1.4 663,882 - -0.6 663,882 - +1.6 663,897 + -1.6 663,897 + +0.6 663,933 + -2.1 663,941 - -0.2 663,969 + -0.4 663,977 - -0.0 664,011 + -1.2 664,038 - Synpcc7942_0667 0.17 +2.2 664,046 - Synpcc7942_0667 0.20 +2.8 664,061 + Synpcc7942_0667 0.24 -0.0 664,100 + Synpcc7942_0667 0.37 +0.6 664,172 + Synpcc7942_0667 0.60 +3.4 664,258 + Synpcc7942_0667 0.88 -1.1 664,306 + -0.7 664,384 + Synpcc7942_0668 0.55 -0.4 664,384 + Synpcc7942_0668 0.55 -2.0 664,384 + Synpcc7942_0668 0.55 -0.3 664,384 + Synpcc7942_0668 0.55 +0.4 664,392 - Synpcc7942_0668 0.61 -2.5 664,393 - Synpcc7942_0668 0.62 -2.1 664,454 + +2.6 664,454 + +1.0 664,454 + -3.0 664,462 - -1.2 664,473 + -1.9 664,478 + -1.7 664,577 + +2.6 664,600 + mpg Synpcc7942_0669 0.10 +1.6 664,618 - mpg Synpcc7942_0669 0.13 -0.1 664,636 + mpg Synpcc7942_0669 0.16 -2.2 664,660 + mpg Synpcc7942_0669 0.21 -0.4 664,695 - -1.9 664,697 + mpg Synpcc7942_0669 0.27 +2.5 664,697 + mpg Synpcc7942_0669 0.27 -0.9 664,699 + mpg Synpcc7942_0669 0.27 +0.6 664,705 - mpg Synpcc7942_0669 0.28 -0.5 664,720 + mpg Synpcc7942_0669 0.31 +1.0 664,745 + mpg Synpcc7942_0669 0.35 -2.8 664,755 + mpg Synpcc7942_0669 0.37 +1.6 664,755 + mpg Synpcc7942_0669 0.37 -0.0 664,755 + mpg Synpcc7942_0669 0.37 -0.8 664,755 + mpg Synpcc7942_0669 0.37 +2.6 664,763 - mpg Synpcc7942_0669 0.38 +1.6 664,763 - mpg Synpcc7942_0669 0.38 +1.6 664,763 - mpg Synpcc7942_0669 0.38 -0.4 664,763 - mpg Synpcc7942_0669 0.38 -0.0 664,780 + mpg Synpcc7942_0669 0.41 -1.2 664,788 - mpg Synpcc7942_0669 0.42 -1.5 664,790 + +1.2 664,824 - mpg Synpcc7942_0669 0.49 -0.0 664,824 - mpg Synpcc7942_0669 0.49 +0.2 664,891 - mpg Synpcc7942_0669 0.60 +1.3 664,891 - mpg Synpcc7942_0669 0.60 -0.9 664,891 - mpg Synpcc7942_0669 0.60 -1.5 664,931 + mpg Synpcc7942_0669 0.67 -0.0 664,931 + mpg Synpcc7942_0669 0.67 -1.5 664,934 - mpg Synpcc7942_0669 0.67 -1.6 664,947 + mpg Synpcc7942_0669 0.70 +0.6 664,947 + mpg Synpcc7942_0669 0.70 -0.4 664,947 + mpg Synpcc7942_0669 0.70 +2.3 665,014 + mpg Synpcc7942_0669 0.81 +2.1 665,014 + mpg Synpcc7942_0669 0.81 -0.7 665,105 + +0.6 665,125 - -1.0
Or see this region's nucleotide sequence