Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ictB and dc13 are separated by 30 nucleotides dc13 and Synpcc7942_0359 are separated by 75 nucleotides
Synpcc7942_0357: ictB - Inorganic carbon transporter, at 349,875 to 351,278
ictB
Synpcc7942_0358: dc13 - tRNA (guanine-N(7))-methyltransferase, at 351,309 to 351,950
dc13
Synpcc7942_0359: Synpcc7942_0359 - hypothetical protein, at 352,026 to 353,207
_0359
Position (kb)
351
352 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 351.282 kb on - strand at 351.287 kb on + strand at 351.299 kb on + strand at 351.300 kb on + strand at 351.305 kb on - strand at 351.307 kb on + strand at 351.331 kb on - strand at 351.340 kb on + strand at 351.343 kb on - strand at 351.456 kb on + strand, within dc13 at 351.505 kb on - strand, within dc13 at 351.506 kb on + strand, within dc13 at 351.514 kb on - strand, within dc13 at 351.529 kb on + strand, within dc13 at 351.613 kb on - strand, within dc13 at 351.618 kb on - strand, within dc13 at 351.618 kb on - strand, within dc13 at 351.678 kb on + strand, within dc13 at 351.704 kb on + strand, within dc13 at 351.704 kb on + strand, within dc13 at 351.710 kb on - strand at 351.788 kb on - strand, within dc13 at 351.788 kb on - strand, within dc13 at 351.789 kb on - strand at 351.867 kb on - strand, within dc13 at 351.867 kb on - strand, within dc13 at 351.867 kb on - strand, within dc13 at 351.869 kb on + strand, within dc13 at 351.874 kb on - strand, within dc13 at 351.893 kb on - strand at 351.894 kb on - strand at 351.927 kb on - strand at 351.935 kb on + strand at 351.960 kb on + strand at 351.981 kb on + strand at 351.983 kb on - strand at 351.986 kb on + strand at 351.989 kb on - strand at 351.999 kb on - strand at 352.011 kb on + strand at 352.019 kb on - strand at 352.031 kb on - strand at 352.052 kb on + strand at 352.052 kb on + strand at 352.052 kb on + strand at 352.060 kb on - strand at 352.060 kb on - strand at 352.074 kb on - strand at 352.126 kb on + strand at 352.145 kb on + strand, within Synpcc7942_0359 at 352.145 kb on + strand, within Synpcc7942_0359 at 352.149 kb on - strand, within Synpcc7942_0359 at 352.180 kb on + strand, within Synpcc7942_0359 at 352.197 kb on + strand, within Synpcc7942_0359 at 352.206 kb on - strand, within Synpcc7942_0359 at 352.259 kb on + strand, within Synpcc7942_0359 at 352.267 kb on - strand, within Synpcc7942_0359 at 352.268 kb on + strand, within Synpcc7942_0359 at 352.268 kb on + strand, within Synpcc7942_0359 at 352.273 kb on + strand, within Synpcc7942_0359 at 352.276 kb on - strand, within Synpcc7942_0359 at 352.277 kb on - strand, within Synpcc7942_0359 at 352.281 kb on - strand, within Synpcc7942_0359 at 352.281 kb on - strand, within Synpcc7942_0359 at 352.324 kb on + strand, within Synpcc7942_0359 at 352.339 kb on + strand, within Synpcc7942_0359 at 352.394 kb on - strand, within Synpcc7942_0359 at 352.402 kb on - strand, within Synpcc7942_0359 at 352.413 kb on + strand, within Synpcc7942_0359 at 352.533 kb on + strand, within Synpcc7942_0359 at 352.533 kb on + strand, within Synpcc7942_0359 at 352.541 kb on - strand, within Synpcc7942_0359 at 352.541 kb on - strand, within Synpcc7942_0359 at 352.546 kb on - strand, within Synpcc7942_0359 at 352.563 kb on + strand, within Synpcc7942_0359 at 352.563 kb on + strand, within Synpcc7942_0359 at 352.563 kb on + strand, within Synpcc7942_0359 at 352.571 kb on - strand, within Synpcc7942_0359 at 352.571 kb on - strand, within Synpcc7942_0359 at 352.590 kb on - strand, within Synpcc7942_0359 at 352.607 kb on + strand, within Synpcc7942_0359 at 352.607 kb on + strand, within Synpcc7942_0359 at 352.664 kb on - strand, within Synpcc7942_0359 at 352.665 kb on + strand, within Synpcc7942_0359 at 352.670 kb on + strand, within Synpcc7942_0359 at 352.683 kb on - strand, within Synpcc7942_0359 at 352.692 kb on + strand, within Synpcc7942_0359 at 352.723 kb on + strand, within Synpcc7942_0359 at 352.728 kb on - strand, within Synpcc7942_0359 at 352.736 kb on - strand, within Synpcc7942_0359 at 352.753 kb on + strand, within Synpcc7942_0359 at 352.754 kb on + strand, within Synpcc7942_0359 at 352.769 kb on + strand, within Synpcc7942_0359 at 352.769 kb on + strand, within Synpcc7942_0359 at 352.777 kb on - strand, within Synpcc7942_0359 at 352.785 kb on - strand, within Synpcc7942_0359 at 352.786 kb on - strand, within Synpcc7942_0359 at 352.874 kb on + strand, within Synpcc7942_0359 at 352.895 kb on + strand, within Synpcc7942_0359 at 352.898 kb on - strand, within Synpcc7942_0359
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 351,282 - +2.2 351,287 + +0.3 351,299 + -1.5 351,300 + +2.6 351,305 - +0.8 351,307 + -0.3 351,331 - +0.3 351,340 + -0.6 351,343 - -1.3 351,456 + dc13 Synpcc7942_0358 0.23 +0.6 351,505 - dc13 Synpcc7942_0358 0.31 -2.4 351,506 + dc13 Synpcc7942_0358 0.31 -0.9 351,514 - dc13 Synpcc7942_0358 0.32 -1.0 351,529 + dc13 Synpcc7942_0358 0.34 -1.0 351,613 - dc13 Synpcc7942_0358 0.47 -3.3 351,618 - dc13 Synpcc7942_0358 0.48 +2.3 351,618 - dc13 Synpcc7942_0358 0.48 -1.0 351,678 + dc13 Synpcc7942_0358 0.57 -1.5 351,704 + dc13 Synpcc7942_0358 0.62 -1.5 351,704 + dc13 Synpcc7942_0358 0.62 +0.2 351,710 - +1.6 351,788 - dc13 Synpcc7942_0358 0.75 -0.7 351,788 - dc13 Synpcc7942_0358 0.75 +0.9 351,789 - -1.0 351,867 - dc13 Synpcc7942_0358 0.87 -1.9 351,867 - dc13 Synpcc7942_0358 0.87 -0.4 351,867 - dc13 Synpcc7942_0358 0.87 +1.6 351,869 + dc13 Synpcc7942_0358 0.87 -2.0 351,874 - dc13 Synpcc7942_0358 0.88 -1.0 351,893 - +1.6 351,894 - +1.6 351,927 - +0.6 351,935 + -0.1 351,960 + -0.4 351,981 + -0.7 351,983 - -1.6 351,986 + -1.6 351,989 - +1.6 351,999 - +1.6 352,011 + -1.0 352,019 - -1.0 352,031 - -1.0 352,052 + -3.3 352,052 + -1.1 352,052 + -1.1 352,060 - +1.3 352,060 - -1.6 352,074 - +0.6 352,126 + -1.9 352,145 + Synpcc7942_0359 0.10 -0.5 352,145 + Synpcc7942_0359 0.10 +2.0 352,149 - Synpcc7942_0359 0.10 -0.6 352,180 + Synpcc7942_0359 0.13 -1.2 352,197 + Synpcc7942_0359 0.14 -2.0 352,206 - Synpcc7942_0359 0.15 -2.7 352,259 + Synpcc7942_0359 0.20 -2.6 352,267 - Synpcc7942_0359 0.20 -0.0 352,268 + Synpcc7942_0359 0.20 -2.5 352,268 + Synpcc7942_0359 0.20 +1.6 352,273 + Synpcc7942_0359 0.21 -2.4 352,276 - Synpcc7942_0359 0.21 +0.6 352,277 - Synpcc7942_0359 0.21 -0.7 352,281 - Synpcc7942_0359 0.22 +2.7 352,281 - Synpcc7942_0359 0.22 -4.2 352,324 + Synpcc7942_0359 0.25 -2.9 352,339 + Synpcc7942_0359 0.26 -2.7 352,394 - Synpcc7942_0359 0.31 +0.7 352,402 - Synpcc7942_0359 0.32 -1.3 352,413 + Synpcc7942_0359 0.33 +1.6 352,533 + Synpcc7942_0359 0.43 -1.1 352,533 + Synpcc7942_0359 0.43 -1.4 352,541 - Synpcc7942_0359 0.44 -0.5 352,541 - Synpcc7942_0359 0.44 -0.7 352,546 - Synpcc7942_0359 0.44 -1.3 352,563 + Synpcc7942_0359 0.45 +0.1 352,563 + Synpcc7942_0359 0.45 -0.9 352,563 + Synpcc7942_0359 0.45 +0.8 352,571 - Synpcc7942_0359 0.46 -0.8 352,571 - Synpcc7942_0359 0.46 -0.5 352,590 - Synpcc7942_0359 0.48 +1.9 352,607 + Synpcc7942_0359 0.49 -0.9 352,607 + Synpcc7942_0359 0.49 +0.0 352,664 - Synpcc7942_0359 0.54 -1.8 352,665 + Synpcc7942_0359 0.54 -1.9 352,670 + Synpcc7942_0359 0.54 +2.0 352,683 - Synpcc7942_0359 0.56 +2.6 352,692 + Synpcc7942_0359 0.56 -2.5 352,723 + Synpcc7942_0359 0.59 +2.6 352,728 - Synpcc7942_0359 0.59 -1.4 352,736 - Synpcc7942_0359 0.60 +1.6 352,753 + Synpcc7942_0359 0.62 +2.9 352,754 + Synpcc7942_0359 0.62 -0.9 352,769 + Synpcc7942_0359 0.63 -3.2 352,769 + Synpcc7942_0359 0.63 -1.6 352,777 - Synpcc7942_0359 0.64 -1.1 352,785 - Synpcc7942_0359 0.64 -0.7 352,786 - Synpcc7942_0359 0.64 +0.6 352,874 + Synpcc7942_0359 0.72 -2.4 352,895 + Synpcc7942_0359 0.74 -1.8 352,898 - Synpcc7942_0359 0.74 +2.1
Or see this region's nucleotide sequence