Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnN and Synpcc7942_0170 are separated by 150 nucleotides Synpcc7942_0170 and cysM are separated by 44 nucleotides
Synpcc7942_0169: glnN - glutamate-ammonia ligase, glutamine synthetase type III, at 168,176 to 170,347
glnN
Synpcc7942_0170: Synpcc7942_0170 - hypothetical protein, at 170,498 to 170,752
_0170
Synpcc7942_0171: cysM - cysteine synthase A, at 170,797 to 171,915
cysM
Position (kb) 170
171 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 169.535 kb on - strand, within glnN at 169.572 kb on - strand, within glnN at 169.580 kb on + strand, within glnN at 169.679 kb on - strand, within glnN at 169.708 kb on + strand, within glnN at 169.716 kb on - strand, within glnN at 169.716 kb on - strand, within glnN at 169.724 kb on - strand, within glnN at 169.729 kb on - strand, within glnN at 169.739 kb on - strand, within glnN at 169.741 kb on - strand, within glnN at 169.741 kb on - strand, within glnN at 169.745 kb on - strand, within glnN at 169.755 kb on - strand, within glnN at 169.755 kb on - strand, within glnN at 169.764 kb on - strand, within glnN at 169.774 kb on - strand at 169.795 kb on + strand, within glnN at 169.795 kb on + strand, within glnN at 169.795 kb on + strand, within glnN at 169.837 kb on + strand, within glnN at 169.845 kb on - strand, within glnN at 169.850 kb on - strand, within glnN at 169.860 kb on + strand, within glnN at 169.879 kb on + strand, within glnN at 169.887 kb on - strand, within glnN at 169.894 kb on - strand, within glnN at 169.904 kb on - strand, within glnN at 169.924 kb on + strand, within glnN at 169.965 kb on - strand, within glnN at 169.965 kb on - strand, within glnN at 169.977 kb on - strand, within glnN at 169.990 kb on + strand, within glnN at 170.053 kb on - strand at 170.073 kb on - strand, within glnN at 170.180 kb on + strand at 170.180 kb on + strand at 170.180 kb on + strand at 170.180 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.221 kb on + strand at 170.224 kb on - strand at 170.224 kb on - strand at 170.258 kb on + strand at 170.265 kb on - strand at 170.271 kb on - strand at 170.330 kb on + strand at 170.342 kb on + strand at 170.348 kb on + strand at 170.350 kb on - strand at 170.350 kb on - strand at 170.360 kb on + strand at 170.368 kb on - strand at 170.390 kb on + strand at 170.457 kb on - strand at 170.459 kb on - strand at 170.468 kb on - strand at 170.468 kb on - strand at 170.475 kb on - strand at 170.480 kb on - strand at 170.501 kb on - strand at 170.508 kb on + strand at 170.532 kb on + strand, within Synpcc7942_0170 at 170.566 kb on - strand, within Synpcc7942_0170 at 170.602 kb on - strand, within Synpcc7942_0170 at 170.646 kb on + strand, within Synpcc7942_0170 at 170.654 kb on - strand, within Synpcc7942_0170 at 170.656 kb on - strand, within Synpcc7942_0170 at 170.670 kb on - strand, within Synpcc7942_0170 at 170.672 kb on - strand, within Synpcc7942_0170 at 170.736 kb on + strand at 170.746 kb on + strand at 170.746 kb on + strand at 170.759 kb on + strand at 170.767 kb on - strand at 170.795 kb on - strand at 170.836 kb on + strand at 170.837 kb on + strand at 170.837 kb on + strand at 170.843 kb on + strand at 170.886 kb on - strand at 170.898 kb on + strand at 170.945 kb on + strand, within cysM at 170.988 kb on - strand, within cysM at 171.007 kb on + strand, within cysM at 171.161 kb on + strand, within cysM at 171.169 kb on - strand, within cysM at 171.174 kb on - strand, within cysM at 171.186 kb on + strand, within cysM at 171.189 kb on - strand, within cysM at 171.228 kb on + strand, within cysM at 171.244 kb on + strand, within cysM at 171.251 kb on + strand, within cysM at 171.274 kb on + strand, within cysM at 171.285 kb on + strand, within cysM at 171.293 kb on - strand, within cysM at 171.337 kb on + strand, within cysM at 171.361 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.559 kb on + strand at 171.559 kb on + strand, within cysM at 171.567 kb on - strand, within cysM at 171.567 kb on - strand, within cysM at 171.627 kb on - strand, within cysM at 171.687 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.695 kb on - strand, within cysM at 171.707 kb on + strand, within cysM at 171.713 kb on + strand, within cysM at 171.725 kb on + strand, within cysM at 171.746 kb on + strand, within cysM
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 169,535 - glnN Synpcc7942_0169 0.63 -2.7 169,572 - glnN Synpcc7942_0169 0.64 +2.1 169,580 + glnN Synpcc7942_0169 0.65 +2.9 169,679 - glnN Synpcc7942_0169 0.69 +1.8 169,708 + glnN Synpcc7942_0169 0.71 -1.0 169,716 - glnN Synpcc7942_0169 0.71 -0.8 169,716 - glnN Synpcc7942_0169 0.71 +2.4 169,724 - glnN Synpcc7942_0169 0.71 +2.9 169,729 - glnN Synpcc7942_0169 0.72 +0.4 169,739 - glnN Synpcc7942_0169 0.72 +1.5 169,741 - glnN Synpcc7942_0169 0.72 +2.5 169,741 - glnN Synpcc7942_0169 0.72 -0.9 169,745 - glnN Synpcc7942_0169 0.72 -0.4 169,755 - glnN Synpcc7942_0169 0.73 -2.0 169,755 - glnN Synpcc7942_0169 0.73 +1.8 169,764 - glnN Synpcc7942_0169 0.73 -0.6 169,774 - -0.3 169,795 + glnN Synpcc7942_0169 0.75 +2.0 169,795 + glnN Synpcc7942_0169 0.75 -2.2 169,795 + glnN Synpcc7942_0169 0.75 -1.7 169,837 + glnN Synpcc7942_0169 0.76 -0.6 169,845 - glnN Synpcc7942_0169 0.77 -1.9 169,850 - glnN Synpcc7942_0169 0.77 +1.4 169,860 + glnN Synpcc7942_0169 0.78 -0.5 169,879 + glnN Synpcc7942_0169 0.78 -0.8 169,887 - glnN Synpcc7942_0169 0.79 +1.5 169,894 - glnN Synpcc7942_0169 0.79 +2.2 169,904 - glnN Synpcc7942_0169 0.80 -3.0 169,924 + glnN Synpcc7942_0169 0.80 -1.7 169,965 - glnN Synpcc7942_0169 0.82 +0.0 169,965 - glnN Synpcc7942_0169 0.82 -0.7 169,977 - glnN Synpcc7942_0169 0.83 -1.1 169,990 + glnN Synpcc7942_0169 0.84 -0.5 170,053 - -0.3 170,073 - glnN Synpcc7942_0169 0.87 -1.3 170,180 + -0.6 170,180 + -0.8 170,180 + -1.7 170,180 + -0.0 170,216 + -1.4 170,216 + +0.7 170,216 + +1.4 170,216 + -0.2 170,216 + +2.1 170,221 + -1.3 170,224 - +1.5 170,224 - +2.2 170,258 + +1.5 170,265 - -2.1 170,271 - -0.6 170,330 + +0.4 170,342 + -0.2 170,348 + +1.0 170,350 - -1.3 170,350 - +0.2 170,360 + +1.5 170,368 - +2.3 170,390 + +2.1 170,457 - -2.8 170,459 - -1.0 170,468 - -3.1 170,468 - +2.5 170,475 - +0.7 170,480 - -0.2 170,501 - -0.5 170,508 + -0.5 170,532 + Synpcc7942_0170 0.13 +1.0 170,566 - Synpcc7942_0170 0.27 -1.7 170,602 - Synpcc7942_0170 0.41 -1.0 170,646 + Synpcc7942_0170 0.58 -0.0 170,654 - Synpcc7942_0170 0.61 -1.9 170,656 - Synpcc7942_0170 0.62 -0.5 170,670 - Synpcc7942_0170 0.67 -1.0 170,672 - Synpcc7942_0170 0.68 -2.3 170,736 + -1.8 170,746 + -0.9 170,746 + +1.1 170,759 + -0.1 170,767 - +2.1 170,795 - -0.0 170,836 + -3.6 170,837 + -0.5 170,837 + -0.0 170,843 + +0.8 170,886 - +1.5 170,898 + +1.0 170,945 + cysM Synpcc7942_0171 0.13 +0.5 170,988 - cysM Synpcc7942_0171 0.17 +1.5 171,007 + cysM Synpcc7942_0171 0.19 +2.1 171,161 + cysM Synpcc7942_0171 0.33 +1.5 171,169 - cysM Synpcc7942_0171 0.33 +0.5 171,174 - cysM Synpcc7942_0171 0.34 +1.5 171,186 + cysM Synpcc7942_0171 0.35 +2.1 171,189 - cysM Synpcc7942_0171 0.35 +0.5 171,228 + cysM Synpcc7942_0171 0.39 +0.8 171,244 + cysM Synpcc7942_0171 0.40 +1.5 171,251 + cysM Synpcc7942_0171 0.41 +0.7 171,274 + cysM Synpcc7942_0171 0.43 +1.5 171,285 + cysM Synpcc7942_0171 0.44 +0.5 171,293 - cysM Synpcc7942_0171 0.44 -1.1 171,337 + cysM Synpcc7942_0171 0.48 -0.8 171,361 + cysM Synpcc7942_0171 0.50 -0.0 171,515 + cysM Synpcc7942_0171 0.64 +0.5 171,515 + cysM Synpcc7942_0171 0.64 +1.5 171,515 + cysM Synpcc7942_0171 0.64 +1.5 171,515 + -0.8 171,523 - cysM Synpcc7942_0171 0.65 +0.5 171,523 - cysM Synpcc7942_0171 0.65 +0.5 171,523 - cysM Synpcc7942_0171 0.65 +1.5 171,523 - cysM Synpcc7942_0171 0.65 +1.5 171,559 + -0.5 171,559 + cysM Synpcc7942_0171 0.68 +0.5 171,567 - cysM Synpcc7942_0171 0.69 +1.5 171,567 - cysM Synpcc7942_0171 0.69 -2.0 171,627 - cysM Synpcc7942_0171 0.74 +0.5 171,687 + cysM Synpcc7942_0171 0.80 +1.5 171,694 + cysM Synpcc7942_0171 0.80 -1.0 171,694 + cysM Synpcc7942_0171 0.80 -2.2 171,695 - cysM Synpcc7942_0171 0.80 +1.5 171,707 + cysM Synpcc7942_0171 0.81 +0.5 171,713 + cysM Synpcc7942_0171 0.82 -0.8 171,725 + cysM Synpcc7942_0171 0.83 +0.5 171,746 + cysM Synpcc7942_0171 0.85 -0.0
Or see this region's nucleotide sequence