Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0121 and tRNA-Cys are separated by 42 nucleotides tRNA-Cys and Synpcc7942_0122 are separated by 478 nucleotides Synpcc7942_0122 and Synpcc7942_0123 are separated by 399 nucleotides Synpcc7942_0123 and tRNA-Cys are separated by 42 nucleotides tRNA-Cys and Synpcc7942_0124 are separated by 56 nucleotides
Synpcc7942_0121: Synpcc7942_0121 - hypothetical protein, at 120,013 to 121,239
_0121
Synpcc7942_R0026: tRNA-Cys - tRNA-Cys, at 121,282 to 121,352
tRNA-Cys
Synpcc7942_0122: Synpcc7942_0122 - EAL, at 121,831 to 123,408
_0122
Synpcc7942_0123: Synpcc7942_0123 - hypothetical protein, at 123,808 to 123,897
_0123
Synpcc7942_R0027: tRNA-Cys - tRNA-Cys, at 123,940 to 124,013
tRNA-Cys
Synpcc7942_0124: Synpcc7942_0124 - thiol methyltransferase 1-like, at 124,070 to 124,669
_0124
Position (kb)
121
122
123
124 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 121.254 kb on + strand at 121.399 kb on - strand at 121.410 kb on + strand at 121.418 kb on - strand at 121.496 kb on + strand at 121.837 kb on - strand at 121.837 kb on - strand at 121.841 kb on + strand at 121.841 kb on + strand at 121.849 kb on - strand at 121.910 kb on + strand at 122.136 kb on - strand, within Synpcc7942_0122 at 122.218 kb on - strand, within Synpcc7942_0122 at 122.249 kb on - strand, within Synpcc7942_0122 at 122.272 kb on - strand, within Synpcc7942_0122 at 122.279 kb on + strand, within Synpcc7942_0122 at 122.311 kb on + strand, within Synpcc7942_0122 at 122.311 kb on + strand, within Synpcc7942_0122 at 122.317 kb on - strand, within Synpcc7942_0122 at 122.319 kb on - strand, within Synpcc7942_0122 at 122.541 kb on + strand, within Synpcc7942_0122 at 122.952 kb on + strand, within Synpcc7942_0122 at 123.037 kb on + strand, within Synpcc7942_0122 at 123.045 kb on - strand, within Synpcc7942_0122 at 123.047 kb on - strand, within Synpcc7942_0122 at 123.058 kb on + strand, within Synpcc7942_0122 at 123.164 kb on + strand, within Synpcc7942_0122 at 123.174 kb on + strand, within Synpcc7942_0122 at 123.187 kb on + strand, within Synpcc7942_0122 at 123.195 kb on - strand, within Synpcc7942_0122 at 123.195 kb on - strand, within Synpcc7942_0122 at 123.195 kb on - strand, within Synpcc7942_0122 at 123.195 kb on - strand, within Synpcc7942_0122 at 123.337 kb on + strand at 123.378 kb on - strand at 123.471 kb on - strand at 123.724 kb on + strand at 123.748 kb on - strand at 123.791 kb on - strand at 123.798 kb on - strand at 123.857 kb on - strand, within Synpcc7942_0123 at 123.886 kb on + strand, within Synpcc7942_0123 at 123.886 kb on + strand, within Synpcc7942_0123 at 123.886 kb on + strand, within Synpcc7942_0123 at 123.894 kb on - strand at 123.924 kb on + strand at 123.932 kb on - strand at 123.942 kb on - strand at 123.962 kb on + strand, within tRNA-Cys at 123.977 kb on + strand, within tRNA-Cys at 124.024 kb on - strand at 124.030 kb on - strand at 124.067 kb on + strand at 124.075 kb on - strand at 124.075 kb on - strand at 124.099 kb on + strand at 124.100 kb on + strand at 124.115 kb on + strand at 124.123 kb on - strand at 124.127 kb on + strand at 124.142 kb on + strand, within Synpcc7942_0124 at 124.150 kb on + strand, within Synpcc7942_0124 at 124.160 kb on + strand, within Synpcc7942_0124 at 124.168 kb on - strand at 124.168 kb on - strand, within Synpcc7942_0124 at 124.168 kb on - strand, within Synpcc7942_0124 at 124.168 kb on - strand, within Synpcc7942_0124 at 124.168 kb on - strand, within Synpcc7942_0124 at 124.183 kb on + strand, within Synpcc7942_0124 at 124.199 kb on + strand, within Synpcc7942_0124 at 124.207 kb on - strand, within Synpcc7942_0124 at 124.207 kb on - strand, within Synpcc7942_0124 at 124.207 kb on - strand, within Synpcc7942_0124 at 124.210 kb on + strand, within Synpcc7942_0124 at 124.210 kb on + strand, within Synpcc7942_0124 at 124.230 kb on + strand, within Synpcc7942_0124 at 124.230 kb on + strand, within Synpcc7942_0124 at 124.230 kb on + strand, within Synpcc7942_0124 at 124.238 kb on - strand, within Synpcc7942_0124 at 124.238 kb on - strand, within Synpcc7942_0124 at 124.286 kb on + strand, within Synpcc7942_0124 at 124.286 kb on + strand, within Synpcc7942_0124 at 124.286 kb on + strand, within Synpcc7942_0124 at 124.302 kb on - strand, within Synpcc7942_0124 at 124.322 kb on + strand, within Synpcc7942_0124 at 124.334 kb on + strand, within Synpcc7942_0124 at 124.334 kb on + strand, within Synpcc7942_0124 at 124.334 kb on + strand, within Synpcc7942_0124 at 124.342 kb on - strand, within Synpcc7942_0124 at 124.344 kb on + strand, within Synpcc7942_0124 at 124.352 kb on - strand, within Synpcc7942_0124 at 124.352 kb on - strand, within Synpcc7942_0124 at 124.352 kb on - strand, within Synpcc7942_0124 at 124.365 kb on + strand, within Synpcc7942_0124 at 124.405 kb on + strand, within Synpcc7942_0124 at 124.405 kb on + strand at 124.407 kb on + strand, within Synpcc7942_0124 at 124.407 kb on + strand, within Synpcc7942_0124
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 121,254 + -0.4 121,399 - -1.6 121,410 + -0.9 121,418 - -0.0 121,496 + -1.3 121,837 - -1.3 121,837 - +1.5 121,841 + -0.3 121,841 + +1.5 121,849 - -0.8 121,910 + -1.3 122,136 - Synpcc7942_0122 0.19 -0.8 122,218 - Synpcc7942_0122 0.25 +2.4 122,249 - Synpcc7942_0122 0.26 -1.3 122,272 - Synpcc7942_0122 0.28 -1.8 122,279 + Synpcc7942_0122 0.28 -1.2 122,311 + Synpcc7942_0122 0.30 +0.5 122,311 + Synpcc7942_0122 0.30 -0.0 122,317 - Synpcc7942_0122 0.31 +2.5 122,319 - Synpcc7942_0122 0.31 -1.0 122,541 + Synpcc7942_0122 0.45 -1.4 122,952 + Synpcc7942_0122 0.71 -0.6 123,037 + Synpcc7942_0122 0.76 -0.9 123,045 - Synpcc7942_0122 0.77 -0.4 123,047 - Synpcc7942_0122 0.77 -1.0 123,058 + Synpcc7942_0122 0.78 +2.9 123,164 + Synpcc7942_0122 0.84 +3.4 123,174 + Synpcc7942_0122 0.85 -0.5 123,187 + Synpcc7942_0122 0.86 +1.5 123,195 - Synpcc7942_0122 0.86 +0.1 123,195 - Synpcc7942_0122 0.86 +3.4 123,195 - Synpcc7942_0122 0.86 +1.8 123,195 - Synpcc7942_0122 0.86 +1.5 123,337 + -1.0 123,378 - -0.8 123,471 - -1.0 123,724 + +0.5 123,748 - -1.8 123,791 - -0.3 123,798 - -1.4 123,857 - Synpcc7942_0123 0.54 -2.3 123,886 + Synpcc7942_0123 0.87 +1.0 123,886 + Synpcc7942_0123 0.87 -0.8 123,886 + Synpcc7942_0123 0.87 -0.2 123,894 - -2.0 123,924 + -1.0 123,932 - -1.5 123,942 - +0.1 123,962 + tRNA-Cys Synpcc7942_R0027 0.30 -0.9 123,977 + tRNA-Cys Synpcc7942_R0027 0.50 +1.5 124,024 - -1.3 124,030 - +1.5 124,067 + -2.2 124,075 - -0.6 124,075 - +2.1 124,099 + -3.0 124,100 + -1.7 124,115 + -2.5 124,123 - +3.5 124,127 + -1.5 124,142 + Synpcc7942_0124 0.12 -0.6 124,150 + Synpcc7942_0124 0.13 -0.4 124,160 + Synpcc7942_0124 0.15 -2.1 124,168 - +1.5 124,168 - Synpcc7942_0124 0.16 +3.1 124,168 - Synpcc7942_0124 0.16 -1.1 124,168 - Synpcc7942_0124 0.16 +1.5 124,168 - Synpcc7942_0124 0.16 +2.5 124,183 + Synpcc7942_0124 0.19 -0.1 124,199 + Synpcc7942_0124 0.21 -0.2 124,207 - Synpcc7942_0124 0.23 -2.2 124,207 - Synpcc7942_0124 0.23 +0.7 124,207 - Synpcc7942_0124 0.23 -0.6 124,210 + Synpcc7942_0124 0.23 +0.5 124,210 + Synpcc7942_0124 0.23 -1.1 124,230 + Synpcc7942_0124 0.27 -1.6 124,230 + Synpcc7942_0124 0.27 -0.0 124,230 + Synpcc7942_0124 0.27 +2.5 124,238 - Synpcc7942_0124 0.28 +1.5 124,238 - Synpcc7942_0124 0.28 -1.5 124,286 + Synpcc7942_0124 0.36 -1.5 124,286 + Synpcc7942_0124 0.36 -1.5 124,286 + Synpcc7942_0124 0.36 -0.0 124,302 - Synpcc7942_0124 0.39 +0.5 124,322 + Synpcc7942_0124 0.42 +1.5 124,334 + Synpcc7942_0124 0.44 -1.2 124,334 + Synpcc7942_0124 0.44 -4.5 124,334 + Synpcc7942_0124 0.44 -1.4 124,342 - Synpcc7942_0124 0.45 -1.1 124,344 + Synpcc7942_0124 0.46 +0.5 124,352 - Synpcc7942_0124 0.47 -0.8 124,352 - Synpcc7942_0124 0.47 -0.5 124,352 - Synpcc7942_0124 0.47 -1.3 124,365 + Synpcc7942_0124 0.49 -1.0 124,405 + Synpcc7942_0124 0.56 -1.8 124,405 + -1.9 124,407 + Synpcc7942_0124 0.56 -2.0 124,407 + Synpcc7942_0124 0.56 +0.3
Or see this region's nucleotide sequence