Experiment: Biofilm in WT Conditioned Media in Flasks with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gatB and Synpcc7942_0119 are separated by 307 nucleotides Synpcc7942_0119 and Synpcc7942_0120 are separated by 215 nucleotides Synpcc7942_0120 and Synpcc7942_0121 are separated by 19 nucleotides
Synpcc7942_0118: gatB - aspartyl/glutamyl-tRNA amidotransferase subunit B, at 116,943 to 118,427
gatB
Synpcc7942_0119: Synpcc7942_0119 - hypothetical protein, at 118,735 to 119,292
_0119
Synpcc7942_0120: Synpcc7942_0120 - Cytosine/adenosine deaminase-like, at 119,508 to 119,993
_0120
Synpcc7942_0121: Synpcc7942_0121 - hypothetical protein, at 120,013 to 121,239
_0121
Position (kb)
118
119
120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 118.422 kb on + strand at 118.460 kb on + strand at 118.460 kb on + strand at 118.460 kb on + strand at 118.460 kb on + strand at 118.468 kb on - strand at 118.479 kb on + strand at 118.491 kb on + strand at 118.535 kb on - strand at 118.566 kb on - strand at 118.570 kb on + strand at 118.571 kb on - strand at 118.575 kb on + strand at 118.606 kb on - strand at 118.608 kb on + strand at 118.686 kb on + strand at 118.734 kb on + strand at 118.739 kb on + strand at 118.740 kb on - strand at 118.765 kb on + strand at 118.783 kb on - strand at 118.797 kb on - strand, within Synpcc7942_0119 at 118.823 kb on + strand, within Synpcc7942_0119 at 118.914 kb on - strand, within Synpcc7942_0119 at 118.921 kb on + strand, within Synpcc7942_0119 at 118.921 kb on + strand, within Synpcc7942_0119 at 118.921 kb on + strand at 118.921 kb on + strand, within Synpcc7942_0119 at 118.943 kb on - strand, within Synpcc7942_0119 at 118.948 kb on + strand, within Synpcc7942_0119 at 118.948 kb on - strand, within Synpcc7942_0119 at 118.951 kb on - strand, within Synpcc7942_0119 at 118.956 kb on - strand, within Synpcc7942_0119 at 118.957 kb on + strand, within Synpcc7942_0119 at 118.965 kb on - strand, within Synpcc7942_0119 at 118.965 kb on - strand, within Synpcc7942_0119 at 118.965 kb on - strand, within Synpcc7942_0119 at 118.978 kb on - strand, within Synpcc7942_0119 at 118.993 kb on + strand, within Synpcc7942_0119 at 118.993 kb on + strand, within Synpcc7942_0119 at 119.071 kb on - strand, within Synpcc7942_0119 at 119.071 kb on - strand, within Synpcc7942_0119 at 119.080 kb on + strand at 119.220 kb on + strand, within Synpcc7942_0119 at 119.230 kb on - strand, within Synpcc7942_0119 at 119.240 kb on + strand at 119.375 kb on + strand at 119.397 kb on - strand at 119.401 kb on + strand at 119.401 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.408 kb on + strand at 119.413 kb on + strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.416 kb on - strand at 119.423 kb on + strand at 119.423 kb on + strand at 119.446 kb on + strand at 119.446 kb on + strand at 119.446 kb on + strand at 119.447 kb on + strand at 119.454 kb on - strand at 119.454 kb on - strand at 119.465 kb on - strand at 119.465 kb on - strand at 119.512 kb on - strand at 119.512 kb on - strand at 119.539 kb on - strand at 119.606 kb on - strand, within Synpcc7942_0120 at 119.631 kb on - strand, within Synpcc7942_0120 at 119.657 kb on - strand, within Synpcc7942_0120 at 119.731 kb on + strand, within Synpcc7942_0120 at 119.739 kb on - strand, within Synpcc7942_0120 at 119.748 kb on + strand, within Synpcc7942_0120 at 119.748 kb on + strand, within Synpcc7942_0120 at 119.748 kb on + strand, within Synpcc7942_0120 at 119.748 kb on + strand, within Synpcc7942_0120 at 119.756 kb on - strand, within Synpcc7942_0120 at 119.756 kb on - strand, within Synpcc7942_0120 at 119.756 kb on - strand, within Synpcc7942_0120 at 119.756 kb on - strand, within Synpcc7942_0120 at 119.756 kb on - strand, within Synpcc7942_0120 at 119.756 kb on - strand, within Synpcc7942_0120 at 119.756 kb on - strand, within Synpcc7942_0120 at 119.787 kb on - strand, within Synpcc7942_0120 at 119.795 kb on - strand, within Synpcc7942_0120 at 119.845 kb on + strand, within Synpcc7942_0120 at 119.845 kb on + strand, within Synpcc7942_0120 at 119.853 kb on - strand, within Synpcc7942_0120 at 119.857 kb on - strand, within Synpcc7942_0120 at 119.882 kb on - strand, within Synpcc7942_0120 at 119.899 kb on - strand, within Synpcc7942_0120 at 119.901 kb on + strand, within Synpcc7942_0120 at 119.901 kb on + strand, within Synpcc7942_0120 at 119.909 kb on - strand, within Synpcc7942_0120 at 119.909 kb on - strand, within Synpcc7942_0120 at 119.995 kb on + strand at 120.014 kb on - strand at 120.046 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm in WT Conditioned Media in Flasks with Spin rep 1 remove 118,422 + +2.8 118,460 + -1.0 118,460 + -1.7 118,460 + -1.9 118,460 + +3.1 118,468 - -0.8 118,479 + -0.3 118,491 + +1.5 118,535 - -1.1 118,566 - +2.6 118,570 + -0.6 118,571 - +1.5 118,575 + +0.5 118,606 - -0.0 118,608 + -0.3 118,686 + +2.3 118,734 + +1.5 118,739 + +0.5 118,740 - -1.6 118,765 + -1.5 118,783 - -1.4 118,797 - Synpcc7942_0119 0.11 +1.5 118,823 + Synpcc7942_0119 0.16 -0.4 118,914 - Synpcc7942_0119 0.32 -0.5 118,921 + Synpcc7942_0119 0.33 +1.5 118,921 + Synpcc7942_0119 0.33 -0.5 118,921 + -0.9 118,921 + Synpcc7942_0119 0.33 +2.5 118,943 - Synpcc7942_0119 0.37 -1.3 118,948 + Synpcc7942_0119 0.38 +0.7 118,948 - Synpcc7942_0119 0.38 -1.0 118,951 - Synpcc7942_0119 0.39 -1.9 118,956 - Synpcc7942_0119 0.40 -1.1 118,957 + Synpcc7942_0119 0.40 +2.2 118,965 - Synpcc7942_0119 0.41 -1.6 118,965 - Synpcc7942_0119 0.41 -1.3 118,965 - Synpcc7942_0119 0.41 -1.4 118,978 - Synpcc7942_0119 0.44 +0.2 118,993 + Synpcc7942_0119 0.46 -1.2 118,993 + Synpcc7942_0119 0.46 -1.0 119,071 - Synpcc7942_0119 0.60 -0.0 119,071 - Synpcc7942_0119 0.60 +1.5 119,080 + -1.3 119,220 + Synpcc7942_0119 0.87 -2.5 119,230 - Synpcc7942_0119 0.89 +0.5 119,240 + -0.0 119,375 + +1.5 119,397 - -0.5 119,401 + -0.0 119,401 + -1.9 119,408 + -3.0 119,408 + -0.5 119,408 + -0.4 119,408 + +0.3 119,408 + +0.5 119,408 + -0.2 119,408 + +1.5 119,408 + +2.0 119,408 + +0.5 119,408 + +1.1 119,408 + -1.0 119,413 + -0.5 119,416 - +2.3 119,416 - -2.5 119,416 - +2.1 119,416 - -0.3 119,416 - +2.1 119,416 - -1.2 119,416 - -1.3 119,416 - -1.0 119,416 - -1.4 119,416 - +0.5 119,416 - -1.6 119,423 + -1.6 119,423 + +1.5 119,446 + -0.7 119,446 + -0.0 119,446 + -0.7 119,447 + +3.1 119,454 - -3.6 119,454 - -0.8 119,465 - -0.1 119,465 - -1.4 119,512 - -1.0 119,512 - -0.0 119,539 - +1.5 119,606 - Synpcc7942_0120 0.20 -0.8 119,631 - Synpcc7942_0120 0.25 +1.5 119,657 - Synpcc7942_0120 0.31 -1.9 119,731 + Synpcc7942_0120 0.46 -2.1 119,739 - Synpcc7942_0120 0.48 -0.2 119,748 + Synpcc7942_0120 0.49 -0.6 119,748 + Synpcc7942_0120 0.49 +1.2 119,748 + Synpcc7942_0120 0.49 -1.0 119,748 + Synpcc7942_0120 0.49 +0.2 119,756 - Synpcc7942_0120 0.51 +0.5 119,756 - Synpcc7942_0120 0.51 -0.4 119,756 - Synpcc7942_0120 0.51 +0.5 119,756 - Synpcc7942_0120 0.51 +2.5 119,756 - Synpcc7942_0120 0.51 -0.5 119,756 - Synpcc7942_0120 0.51 -1.0 119,756 - Synpcc7942_0120 0.51 -2.2 119,787 - Synpcc7942_0120 0.57 +0.5 119,795 - Synpcc7942_0120 0.59 -1.7 119,845 + Synpcc7942_0120 0.69 +3.5 119,845 + Synpcc7942_0120 0.69 -0.0 119,853 - Synpcc7942_0120 0.71 -2.9 119,857 - Synpcc7942_0120 0.72 +1.5 119,882 - Synpcc7942_0120 0.77 +0.8 119,899 - Synpcc7942_0120 0.80 +0.4 119,901 + Synpcc7942_0120 0.81 -0.3 119,901 + Synpcc7942_0120 0.81 -1.5 119,909 - Synpcc7942_0120 0.83 -2.1 119,909 - Synpcc7942_0120 0.83 +1.5 119,995 + -0.0 120,014 - -0.0 120,046 - -0.8
Or see this region's nucleotide sequence