Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06665 and MMP_RS06670 are separated by 31 nucleotides MMP_RS06670 and MMP_RS06675 are separated by 24 nucleotides
MMP_RS06665: MMP_RS06665 - glycogen/starch synthase, at 1,278,266 to 1,279,834
_RS06665
MMP_RS06670: MMP_RS06670 - glucose-6-phosphate isomerase, at 1,279,866 to 1,281,182
_RS06670
MMP_RS06675: MMP_RS06675 - ADP-specific phosphofructokinase, at 1,281,207 to 1,282,598
_RS06675
Position (kb)
1279
1280
1281
1282 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1278.987 kb on + strand, within MMP_RS06665 at 1279.004 kb on + strand, within MMP_RS06665 at 1279.024 kb on + strand, within MMP_RS06665 at 1279.135 kb on + strand, within MMP_RS06665 at 1279.273 kb on + strand, within MMP_RS06665 at 1279.382 kb on - strand, within MMP_RS06665 at 1279.552 kb on + strand, within MMP_RS06665 at 1279.552 kb on + strand, within MMP_RS06665 at 1279.582 kb on - strand, within MMP_RS06665 at 1279.880 kb on + strand at 1279.891 kb on + strand at 1279.932 kb on + strand at 1280.044 kb on - strand, within MMP_RS06670 at 1280.048 kb on - strand, within MMP_RS06670 at 1280.053 kb on + strand, within MMP_RS06670 at 1280.149 kb on - strand, within MMP_RS06670 at 1280.191 kb on + strand, within MMP_RS06670 at 1280.195 kb on - strand, within MMP_RS06670 at 1280.541 kb on + strand, within MMP_RS06670 at 1280.612 kb on - strand, within MMP_RS06670 at 1280.612 kb on - strand, within MMP_RS06670 at 1280.784 kb on - strand, within MMP_RS06670 at 1280.828 kb on + strand, within MMP_RS06670 at 1280.837 kb on - strand, within MMP_RS06670 at 1280.837 kb on - strand, within MMP_RS06670 at 1280.838 kb on + strand, within MMP_RS06670 at 1280.838 kb on + strand, within MMP_RS06670 at 1281.148 kb on + strand at 1281.150 kb on + strand at 1281.151 kb on - strand at 1281.152 kb on + strand at 1281.162 kb on - strand at 1281.169 kb on - strand at 1281.170 kb on + strand at 1281.170 kb on + strand at 1281.218 kb on - strand at 1281.219 kb on + strand at 1281.257 kb on + strand at 1281.261 kb on - strand at 1281.261 kb on - strand at 1281.261 kb on - strand at 1281.262 kb on + strand at 1281.319 kb on - strand at 1281.496 kb on + strand, within MMP_RS06675 at 1281.496 kb on + strand, within MMP_RS06675 at 1281.949 kb on - strand, within MMP_RS06675
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 1,278,987 + MMP_RS06665 0.46 -0.6 1,279,004 + MMP_RS06665 0.47 +0.5 1,279,024 + MMP_RS06665 0.48 +0.1 1,279,135 + MMP_RS06665 0.55 -0.2 1,279,273 + MMP_RS06665 0.64 +0.2 1,279,382 - MMP_RS06665 0.71 -1.1 1,279,552 + MMP_RS06665 0.82 +0.8 1,279,552 + MMP_RS06665 0.82 -0.1 1,279,582 - MMP_RS06665 0.84 -0.8 1,279,880 + -3.9 1,279,891 + -2.4 1,279,932 + -3.7 1,280,044 - MMP_RS06670 0.14 -4.3 1,280,048 - MMP_RS06670 0.14 -2.0 1,280,053 + MMP_RS06670 0.14 -3.4 1,280,149 - MMP_RS06670 0.21 -1.6 1,280,191 + MMP_RS06670 0.25 -4.1 1,280,195 - MMP_RS06670 0.25 -3.1 1,280,541 + MMP_RS06670 0.51 -2.6 1,280,612 - MMP_RS06670 0.57 -4.1 1,280,612 - MMP_RS06670 0.57 -1.7 1,280,784 - MMP_RS06670 0.70 -1.8 1,280,828 + MMP_RS06670 0.73 -2.2 1,280,837 - MMP_RS06670 0.74 -5.6 1,280,837 - MMP_RS06670 0.74 -3.5 1,280,838 + MMP_RS06670 0.74 -1.4 1,280,838 + MMP_RS06670 0.74 -4.0 1,281,148 + -0.1 1,281,150 + +0.4 1,281,151 - -0.2 1,281,152 + +1.0 1,281,162 - -0.7 1,281,169 - +0.7 1,281,170 + -0.5 1,281,170 + +1.1 1,281,218 - -1.1 1,281,219 + -0.3 1,281,257 + -0.0 1,281,261 - -1.4 1,281,261 - -0.5 1,281,261 - -0.8 1,281,262 + +0.1 1,281,319 - -1.0 1,281,496 + MMP_RS06675 0.21 +0.3 1,281,496 + MMP_RS06675 0.21 -0.8 1,281,949 - MMP_RS06675 0.53 -0.5
Or see this region's nucleotide sequence