Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06340 and MMP_RS06345 are separated by 52 nucleotides MMP_RS06345 and MMP_RS06350 are separated by 79 nucleotides MMP_RS06350 and MMP_RS06355 overlap by 8 nucleotides MMP_RS06355 and MMP_RS06360 are separated by 38 nucleotides
MMP_RS06340: MMP_RS06340 - phosphoadenosine phosphosulfate reductase family protein, at 1,219,930 to 1,220,820
_RS06340
MMP_RS06345: MMP_RS06345 - formate dehydrogenase accessory sulfurtransferase FdhD, at 1,220,873 to 1,221,574
_RS06345
MMP_RS06350: MMP_RS06350 - HesA/MoeB/ThiF family protein, at 1,221,654 to 1,222,373
_RS06350
MMP_RS06355: MMP_RS06355 - molybdenum cofactor biosynthesis protein MoaE, at 1,222,366 to 1,222,791
_RS06355
MMP_RS06360: MMP_RS06360 - UPF0280 family protein, at 1,222,830 to 1,223,573
_RS06360
Position (kb) 1221
1222
1223 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1220.714 kb on + strand, within MMP_RS06340 at 1220.761 kb on - strand at 1220.822 kb on - strand at 1220.847 kb on + strand at 1220.857 kb on + strand at 1220.888 kb on - strand at 1220.898 kb on + strand at 1220.907 kb on - strand at 1220.914 kb on - strand at 1221.002 kb on + strand, within MMP_RS06345 at 1221.004 kb on - strand, within MMP_RS06345 at 1221.268 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.333 kb on - strand, within MMP_RS06345 at 1221.334 kb on + strand, within MMP_RS06345 at 1221.334 kb on + strand, within MMP_RS06345 at 1221.334 kb on + strand, within MMP_RS06345 at 1221.336 kb on + strand, within MMP_RS06345 at 1221.351 kb on + strand, within MMP_RS06345 at 1221.356 kb on + strand, within MMP_RS06345 at 1221.384 kb on - strand, within MMP_RS06345 at 1221.384 kb on - strand, within MMP_RS06345 at 1221.543 kb on - strand at 1221.599 kb on + strand at 1221.610 kb on - strand at 1221.610 kb on - strand at 1221.611 kb on + strand at 1221.628 kb on + strand at 1221.630 kb on + strand at 1221.641 kb on - strand at 1221.641 kb on - strand at 1221.642 kb on + strand at 1221.642 kb on + strand at 1221.654 kb on + strand at 1221.654 kb on + strand at 1221.654 kb on + strand at 1221.781 kb on + strand, within MMP_RS06350 at 1221.860 kb on + strand, within MMP_RS06350 at 1221.927 kb on + strand, within MMP_RS06350 at 1222.030 kb on + strand, within MMP_RS06350 at 1222.041 kb on + strand, within MMP_RS06350 at 1222.053 kb on + strand, within MMP_RS06350 at 1222.156 kb on - strand, within MMP_RS06350 at 1222.652 kb on - strand, within MMP_RS06355 at 1222.653 kb on + strand, within MMP_RS06355 at 1222.653 kb on + strand, within MMP_RS06355 at 1222.800 kb on + strand at 1222.827 kb on + strand at 1222.926 kb on + strand at 1222.982 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 1,220,714 + MMP_RS06340 0.88 -0.9 1,220,761 - -2.4 1,220,822 - -2.3 1,220,847 + -0.4 1,220,857 + -0.4 1,220,888 - -1.6 1,220,898 + +0.6 1,220,907 - +0.6 1,220,914 - +0.9 1,221,002 + MMP_RS06345 0.18 +0.1 1,221,004 - MMP_RS06345 0.19 +0.5 1,221,268 - MMP_RS06345 0.56 -0.6 1,221,333 - MMP_RS06345 0.66 +0.1 1,221,333 - MMP_RS06345 0.66 -0.2 1,221,333 - MMP_RS06345 0.66 -0.2 1,221,333 - MMP_RS06345 0.66 +0.8 1,221,333 - MMP_RS06345 0.66 +0.8 1,221,333 - MMP_RS06345 0.66 +0.6 1,221,334 + MMP_RS06345 0.66 +0.5 1,221,334 + MMP_RS06345 0.66 +0.1 1,221,334 + MMP_RS06345 0.66 -0.2 1,221,336 + MMP_RS06345 0.66 +0.3 1,221,351 + MMP_RS06345 0.68 -0.2 1,221,356 + MMP_RS06345 0.69 -0.4 1,221,384 - MMP_RS06345 0.73 -0.4 1,221,384 - MMP_RS06345 0.73 +0.0 1,221,543 - +0.4 1,221,599 + +0.5 1,221,610 - +0.4 1,221,610 - +0.5 1,221,611 + -1.1 1,221,628 + +0.5 1,221,630 + +0.3 1,221,641 - +0.4 1,221,641 - -0.3 1,221,642 + +0.4 1,221,642 + +1.0 1,221,654 + -1.5 1,221,654 + -2.9 1,221,654 + -4.5 1,221,781 + MMP_RS06350 0.18 -3.5 1,221,860 + MMP_RS06350 0.29 -3.7 1,221,927 + MMP_RS06350 0.38 -2.4 1,222,030 + MMP_RS06350 0.52 -3.6 1,222,041 + MMP_RS06350 0.54 -2.5 1,222,053 + MMP_RS06350 0.55 -4.0 1,222,156 - MMP_RS06350 0.70 -4.0 1,222,652 - MMP_RS06355 0.67 -2.4 1,222,653 + MMP_RS06355 0.67 -2.7 1,222,653 + MMP_RS06355 0.67 -2.8 1,222,800 + +0.3 1,222,827 + -1.3 1,222,926 + -3.5 1,222,982 - -3.7
Or see this region's nucleotide sequence