Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04950 and MMP_RS04955 are separated by 46 nucleotides MMP_RS04955 and MMP_RS04960 are separated by 1 nucleotides MMP_RS04960 and MMP_RS04965 are separated by 19 nucleotides
MMP_RS04950: MMP_RS04950 - succinate--CoA ligase subunit alpha, at 942,855 to 943,718
_RS04950
MMP_RS04955: MMP_RS04955 - DNA topoisomerase I, at 943,765 to 946,236
_RS04955
MMP_RS04960: MMP_RS04960 - cytochrome c biogenesis CcdA family protein, at 946,238 to 946,891
_RS04960
MMP_RS04965: MMP_RS04965 - thioredoxin family protein, at 946,911 to 947,258
_RS04965
Position (kb) 943
944
945
946
947 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 942.772 kb on - strand at 942.773 kb on + strand at 942.775 kb on + strand at 942.776 kb on - strand at 942.776 kb on - strand at 942.777 kb on + strand at 942.787 kb on + strand at 942.825 kb on + strand at 942.841 kb on + strand at 943.069 kb on - strand at 943.099 kb on + strand at 943.106 kb on - strand at 943.277 kb on + strand at 943.697 kb on + strand at 943.717 kb on + strand at 943.751 kb on + strand at 943.776 kb on + strand at 943.890 kb on - strand at 943.891 kb on + strand at 943.893 kb on + strand at 943.899 kb on + strand at 943.919 kb on - strand at 943.921 kb on - strand at 944.029 kb on + strand, within MMP_RS04955 at 944.279 kb on - strand, within MMP_RS04955 at 944.310 kb on + strand, within MMP_RS04955 at 944.453 kb on - strand, within MMP_RS04955 at 944.502 kb on - strand, within MMP_RS04955 at 944.737 kb on - strand, within MMP_RS04955 at 944.737 kb on - strand, within MMP_RS04955 at 944.738 kb on + strand, within MMP_RS04955 at 944.738 kb on + strand, within MMP_RS04955 at 944.738 kb on + strand, within MMP_RS04955 at 944.738 kb on + strand, within MMP_RS04955 at 944.738 kb on + strand, within MMP_RS04955 at 944.789 kb on + strand, within MMP_RS04955 at 944.907 kb on + strand, within MMP_RS04955 at 945.508 kb on + strand, within MMP_RS04955 at 945.726 kb on + strand, within MMP_RS04955 at 945.774 kb on - strand, within MMP_RS04955 at 945.894 kb on - strand, within MMP_RS04955 at 945.895 kb on + strand, within MMP_RS04955 at 945.899 kb on + strand, within MMP_RS04955 at 945.899 kb on + strand, within MMP_RS04955 at 945.976 kb on + strand, within MMP_RS04955 at 946.035 kb on - strand at 946.080 kb on - strand at 946.120 kb on + strand at 946.150 kb on + strand at 946.155 kb on - strand at 946.234 kb on - strand at 946.246 kb on - strand at 946.247 kb on + strand at 946.247 kb on + strand at 946.250 kb on - strand at 946.250 kb on - strand at 946.251 kb on + strand at 946.253 kb on - strand at 946.277 kb on - strand at 946.277 kb on - strand at 946.279 kb on - strand at 946.279 kb on - strand at 946.280 kb on + strand at 946.280 kb on + strand at 946.291 kb on - strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.292 kb on + strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.293 kb on - strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.294 kb on + strand at 946.298 kb on - strand at 946.299 kb on + strand at 946.300 kb on - strand at 946.301 kb on + strand at 946.301 kb on + strand at 946.316 kb on - strand, within MMP_RS04960 at 946.328 kb on - strand, within MMP_RS04960 at 946.328 kb on - strand, within MMP_RS04960 at 946.479 kb on + strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.480 kb on - strand, within MMP_RS04960 at 946.481 kb on + strand, within MMP_RS04960 at 946.539 kb on + strand, within MMP_RS04960 at 946.600 kb on - strand, within MMP_RS04960 at 946.601 kb on + strand, within MMP_RS04960 at 946.619 kb on + strand, within MMP_RS04960 at 946.619 kb on + strand, within MMP_RS04960 at 946.622 kb on - strand, within MMP_RS04960 at 946.623 kb on + strand, within MMP_RS04960 at 946.764 kb on + strand, within MMP_RS04960 at 946.766 kb on - strand, within MMP_RS04960 at 946.917 kb on + strand at 947.070 kb on - strand, within MMP_RS04965 at 947.071 kb on + strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.072 kb on - strand, within MMP_RS04965 at 947.073 kb on + strand, within MMP_RS04965 at 947.073 kb on + strand, within MMP_RS04965 at 947.102 kb on - strand, within MMP_RS04965 at 947.201 kb on - strand, within MMP_RS04965 at 947.221 kb on + strand, within MMP_RS04965 at 947.226 kb on - strand at 947.229 kb on + strand at 947.229 kb on + strand at 947.232 kb on - strand at 947.233 kb on + strand at 947.233 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 942,772 - -1.8 942,773 + +0.1 942,775 + -1.6 942,776 - -2.3 942,776 - -3.4 942,777 + -1.9 942,787 + -3.4 942,825 + -2.8 942,841 + -3.3 943,069 - -3.2 943,099 + -2.4 943,106 - -2.6 943,277 + -2.3 943,697 + -3.0 943,717 + -0.8 943,751 + -0.9 943,776 + -0.1 943,890 - -0.3 943,891 + -4.0 943,893 + -3.2 943,899 + -2.7 943,919 - -1.2 943,921 - -2.6 944,029 + MMP_RS04955 0.11 -0.5 944,279 - MMP_RS04955 0.21 -2.1 944,310 + MMP_RS04955 0.22 +0.4 944,453 - MMP_RS04955 0.28 -3.2 944,502 - MMP_RS04955 0.30 -0.7 944,737 - MMP_RS04955 0.39 -2.0 944,737 - MMP_RS04955 0.39 -3.2 944,738 + MMP_RS04955 0.39 -2.8 944,738 + MMP_RS04955 0.39 -1.1 944,738 + MMP_RS04955 0.39 -3.2 944,738 + MMP_RS04955 0.39 -1.0 944,738 + MMP_RS04955 0.39 -1.9 944,789 + MMP_RS04955 0.41 -3.7 944,907 + MMP_RS04955 0.46 -1.2 945,508 + MMP_RS04955 0.71 -2.3 945,726 + MMP_RS04955 0.79 -2.8 945,774 - MMP_RS04955 0.81 -0.1 945,894 - MMP_RS04955 0.86 -1.5 945,895 + MMP_RS04955 0.86 -1.9 945,899 + MMP_RS04955 0.86 -0.7 945,899 + MMP_RS04955 0.86 -0.0 945,976 + MMP_RS04955 0.89 +0.3 946,035 - +0.5 946,080 - +0.2 946,120 + +0.3 946,150 + +0.5 946,155 - +0.4 946,234 - +0.5 946,246 - +0.4 946,247 + -0.5 946,247 + +1.5 946,250 - +1.0 946,250 - -0.5 946,251 + +0.3 946,253 - +0.2 946,277 - +0.2 946,277 - +0.2 946,279 - -0.4 946,279 - +0.8 946,280 + -1.3 946,280 + -0.3 946,291 - -0.2 946,292 + +0.0 946,292 + -0.2 946,292 + +0.1 946,292 + +0.2 946,293 - +0.5 946,293 - -0.5 946,293 - +0.0 946,293 - -0.0 946,293 - +0.3 946,293 - -0.0 946,293 - -0.2 946,294 + -0.3 946,294 + -0.2 946,294 + -0.7 946,294 + +0.3 946,294 + -0.2 946,298 - -0.3 946,299 + +0.3 946,300 - -0.3 946,301 + +0.3 946,301 + +0.3 946,316 - MMP_RS04960 0.12 +0.1 946,328 - MMP_RS04960 0.14 +0.4 946,328 - MMP_RS04960 0.14 +0.6 946,479 + MMP_RS04960 0.37 +0.1 946,480 - MMP_RS04960 0.37 +0.6 946,480 - MMP_RS04960 0.37 +0.4 946,480 - MMP_RS04960 0.37 +0.3 946,481 + MMP_RS04960 0.37 -0.1 946,539 + MMP_RS04960 0.46 -0.7 946,600 - MMP_RS04960 0.55 +0.2 946,601 + MMP_RS04960 0.56 -0.1 946,619 + MMP_RS04960 0.58 +0.0 946,619 + MMP_RS04960 0.58 -1.8 946,622 - MMP_RS04960 0.59 -0.3 946,623 + MMP_RS04960 0.59 +1.4 946,764 + MMP_RS04960 0.80 +0.2 946,766 - MMP_RS04960 0.81 +0.2 946,917 + +0.4 947,070 - MMP_RS04965 0.46 +0.5 947,071 + MMP_RS04965 0.46 +0.7 947,072 - MMP_RS04965 0.46 -0.1 947,072 - MMP_RS04965 0.46 +0.1 947,072 - MMP_RS04965 0.46 +0.6 947,072 - MMP_RS04965 0.46 -0.1 947,072 - MMP_RS04965 0.46 +0.2 947,072 - MMP_RS04965 0.46 +1.3 947,072 - MMP_RS04965 0.46 +0.6 947,073 + MMP_RS04965 0.47 +0.1 947,073 + MMP_RS04965 0.47 -0.6 947,102 - MMP_RS04965 0.55 -0.8 947,201 - MMP_RS04965 0.83 -0.1 947,221 + MMP_RS04965 0.89 -0.4 947,226 - +0.4 947,229 + +1.2 947,229 + +0.1 947,232 - -0.1 947,233 + -0.2 947,233 + +0.1
Or see this region's nucleotide sequence