Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03635 and MMP_RS03640 are separated by 134 nucleotides MMP_RS03640 and MMP_RS03645 are separated by 95 nucleotides
MMP_RS03635: MMP_RS03635 - beta-CASP ribonuclease aCPSF1, at 682,145 to 684,052
_RS03635
MMP_RS03640: MMP_RS03640 - archaeal proteasome endopeptidase complex subunit beta, at 684,187 to 684,846
_RS03640
MMP_RS03645: MMP_RS03645 - proline--tRNA ligase, at 684,942 to 686,324
_RS03645
Position (kb) 684
685 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 684.066 kb on + strand at 684.072 kb on + strand at 684.147 kb on - strand at 684.148 kb on + strand at 684.172 kb on - strand at 684.272 kb on + strand, within MMP_RS03640 at 684.293 kb on - strand, within MMP_RS03640 at 684.293 kb on - strand, within MMP_RS03640 at 684.552 kb on - strand, within MMP_RS03640 at 684.631 kb on - strand, within MMP_RS03640 at 684.631 kb on - strand, within MMP_RS03640 at 684.682 kb on - strand, within MMP_RS03640 at 684.686 kb on - strand, within MMP_RS03640 at 684.860 kb on + strand at 684.860 kb on + strand at 684.862 kb on - strand at 684.875 kb on + strand at 684.893 kb on + strand at 684.943 kb on - strand at 684.944 kb on + strand at 684.944 kb on + strand at 684.966 kb on + strand at 684.983 kb on + strand at 685.193 kb on + strand at 685.699 kb on - strand at 685.701 kb on - strand at 685.772 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 684,066 + -3.3 684,072 + -3.9 684,147 - -2.9 684,148 + -2.2 684,172 - -1.8 684,272 + MMP_RS03640 0.13 -2.8 684,293 - MMP_RS03640 0.16 -2.7 684,293 - MMP_RS03640 0.16 -3.9 684,552 - MMP_RS03640 0.55 -1.8 684,631 - MMP_RS03640 0.67 -2.0 684,631 - MMP_RS03640 0.67 -2.3 684,682 - MMP_RS03640 0.75 -2.7 684,686 - MMP_RS03640 0.76 -1.0 684,860 + -1.3 684,860 + -2.1 684,862 - -1.4 684,875 + -2.2 684,893 + -1.1 684,943 - +0.0 684,944 + +0.6 684,944 + -0.4 684,966 + -3.4 684,983 + -1.3 685,193 + -3.0 685,699 - -3.1 685,701 - -2.4 685,772 + -2.6
Or see this region's nucleotide sequence