Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03090 and MMP_RS03095 are separated by 116 nucleotides MMP_RS03095 and MMP_RS03100 are separated by 5 nucleotides MMP_RS03100 and MMP_RS03105 are separated by 261 nucleotides
MMP_RS03090: MMP_RS03090 - cation:proton antiporter, at 583,278 to 584,447
_RS03090
MMP_RS03095: MMP_RS03095 - diphthine synthase, at 584,564 to 585,331
_RS03095
MMP_RS03100: MMP_RS03100 - hypothetical protein, at 585,337 to 585,537
_RS03100
MMP_RS03105: MMP_RS03105 - radical SAM protein, at 585,799 to 587,331
_RS03105
Position (kb) 584
585
586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 583.775 kb on - strand, within MMP_RS03090 at 583.786 kb on + strand, within MMP_RS03090 at 583.786 kb on + strand, within MMP_RS03090 at 583.792 kb on + strand, within MMP_RS03090 at 583.843 kb on + strand, within MMP_RS03090 at 583.845 kb on - strand, within MMP_RS03090 at 583.849 kb on + strand, within MMP_RS03090 at 583.889 kb on - strand, within MMP_RS03090 at 584.135 kb on + strand, within MMP_RS03090 at 584.468 kb on - strand at 584.468 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.514 kb on - strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.515 kb on + strand at 584.516 kb on - strand at 584.516 kb on - strand at 584.516 kb on - strand at 584.517 kb on + strand at 584.518 kb on - strand at 584.518 kb on - strand at 584.519 kb on + strand at 584.519 kb on + strand at 584.522 kb on + strand at 584.540 kb on + strand at 584.546 kb on - strand at 584.547 kb on + strand at 584.592 kb on + strand at 584.609 kb on + strand at 584.609 kb on + strand at 584.673 kb on - strand, within MMP_RS03095 at 584.678 kb on + strand, within MMP_RS03095 at 584.678 kb on + strand, within MMP_RS03095 at 584.725 kb on + strand, within MMP_RS03095 at 584.754 kb on + strand, within MMP_RS03095 at 585.002 kb on - strand, within MMP_RS03095 at 585.071 kb on + strand, within MMP_RS03095 at 585.206 kb on - strand, within MMP_RS03095 at 585.210 kb on + strand, within MMP_RS03095 at 585.401 kb on + strand, within MMP_RS03100 at 585.509 kb on + strand, within MMP_RS03100 at 585.512 kb on - strand, within MMP_RS03100 at 585.512 kb on - strand, within MMP_RS03100 at 585.513 kb on + strand, within MMP_RS03100 at 585.514 kb on - strand, within MMP_RS03100 at 585.551 kb on - strand at 585.553 kb on - strand at 585.553 kb on - strand at 585.553 kb on - strand at 585.554 kb on + strand at 585.572 kb on - strand at 585.573 kb on + strand at 585.587 kb on - strand at 585.592 kb on + strand at 585.627 kb on + strand at 585.675 kb on + strand at 585.739 kb on - strand at 585.739 kb on - strand at 585.741 kb on - strand at 585.741 kb on - strand at 585.756 kb on - strand at 585.757 kb on + strand at 585.757 kb on + strand at 585.763 kb on - strand at 585.763 kb on - strand at 585.764 kb on + strand at 585.764 kb on + strand at 585.766 kb on + strand at 585.766 kb on + strand at 585.824 kb on + strand at 585.936 kb on - strand at 585.979 kb on - strand, within MMP_RS03105 at 585.980 kb on + strand, within MMP_RS03105 at 585.986 kb on - strand, within MMP_RS03105 at 585.987 kb on + strand, within MMP_RS03105 at 585.990 kb on - strand, within MMP_RS03105 at 586.117 kb on + strand, within MMP_RS03105
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 583,775 - MMP_RS03090 0.42 +0.1 583,786 + MMP_RS03090 0.43 +0.4 583,786 + MMP_RS03090 0.43 +0.4 583,792 + MMP_RS03090 0.44 +0.3 583,843 + MMP_RS03090 0.48 +1.2 583,845 - MMP_RS03090 0.48 +0.6 583,849 + MMP_RS03090 0.49 +0.7 583,889 - MMP_RS03090 0.52 +0.5 584,135 + MMP_RS03090 0.73 -0.7 584,468 - +1.4 584,468 - +1.2 584,514 - +0.9 584,514 - +0.1 584,514 - -0.4 584,514 - +0.7 584,514 - +1.4 584,514 - +1.2 584,514 - -0.3 584,514 - +0.4 584,514 - +0.5 584,514 - +1.2 584,515 + -0.8 584,515 + +0.9 584,515 + +2.0 584,515 + +0.5 584,515 + +0.8 584,515 + +0.1 584,515 + -1.5 584,515 + +0.8 584,516 - +0.2 584,516 - -0.8 584,516 - -0.1 584,517 + +0.8 584,518 - -2.0 584,518 - -1.8 584,519 + -0.7 584,519 + -0.2 584,522 + -0.4 584,540 + -1.3 584,546 - -2.1 584,547 + -0.9 584,592 + -1.5 584,609 + -1.4 584,609 + -1.5 584,673 - MMP_RS03095 0.14 -3.1 584,678 + MMP_RS03095 0.15 -2.4 584,678 + MMP_RS03095 0.15 -1.7 584,725 + MMP_RS03095 0.21 -2.0 584,754 + MMP_RS03095 0.25 -3.0 585,002 - MMP_RS03095 0.57 -1.0 585,071 + MMP_RS03095 0.66 -1.4 585,206 - MMP_RS03095 0.84 -2.1 585,210 + MMP_RS03095 0.84 -3.3 585,401 + MMP_RS03100 0.32 -0.9 585,509 + MMP_RS03100 0.86 +0.6 585,512 - MMP_RS03100 0.87 -2.4 585,512 - MMP_RS03100 0.87 +0.1 585,513 + MMP_RS03100 0.88 +1.3 585,514 - MMP_RS03100 0.88 +0.8 585,551 - +1.0 585,553 - +1.0 585,553 - -0.1 585,553 - -0.2 585,554 + -0.1 585,572 - +0.2 585,573 + -0.4 585,587 - -0.5 585,592 + -0.0 585,627 + +0.7 585,675 + +0.0 585,739 - +0.2 585,739 - -0.2 585,741 - -0.2 585,741 - -0.0 585,756 - +0.2 585,757 + -0.2 585,757 + +0.2 585,763 - -0.2 585,763 - +0.4 585,764 + -1.3 585,764 + -0.3 585,766 + -0.6 585,766 + -1.3 585,824 + +0.2 585,936 - -1.1 585,979 - MMP_RS03105 0.12 +0.3 585,980 + MMP_RS03105 0.12 +0.3 585,986 - MMP_RS03105 0.12 -0.7 585,987 + MMP_RS03105 0.12 -0.9 585,990 - MMP_RS03105 0.12 -0.1 586,117 + MMP_RS03105 0.21 -0.4
Or see this region's nucleotide sequence