Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01375 and MMP_RS01380 are separated by 20 nucleotides MMP_RS01380 and MMP_RS01385 are separated by 35 nucleotides MMP_RS01385 and MMP_RS01390 are separated by 11 nucleotides
MMP_RS01375: MMP_RS01375 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 255,837 to 257,360
_RS01375
MMP_RS01380: MMP_RS01380 - PHP domain-containing protein, at 257,381 to 258,280
_RS01380
MMP_RS01385: MMP_RS01385 - imidazole glycerol phosphate synthase subunit HisH, at 258,316 to 258,927
_RS01385
MMP_RS01390: MMP_RS01390 - TATA-box-binding protein, at 258,939 to 259,484
_RS01390
Position (kb) 257
258
259 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 256.402 kb on - strand, within MMP_RS01375 at 256.418 kb on - strand, within MMP_RS01375 at 256.419 kb on + strand, within MMP_RS01375 at 256.450 kb on - strand, within MMP_RS01375 at 256.451 kb on + strand, within MMP_RS01375 at 256.455 kb on + strand, within MMP_RS01375 at 256.455 kb on + strand, within MMP_RS01375 at 256.490 kb on - strand, within MMP_RS01375 at 256.492 kb on - strand, within MMP_RS01375 at 256.493 kb on + strand, within MMP_RS01375 at 256.561 kb on + strand, within MMP_RS01375 at 256.796 kb on + strand, within MMP_RS01375 at 256.807 kb on + strand, within MMP_RS01375 at 256.809 kb on + strand, within MMP_RS01375 at 256.809 kb on + strand, within MMP_RS01375 at 256.825 kb on - strand, within MMP_RS01375 at 256.834 kb on - strand, within MMP_RS01375 at 256.874 kb on - strand, within MMP_RS01375 at 256.888 kb on - strand, within MMP_RS01375 at 256.912 kb on - strand, within MMP_RS01375 at 256.921 kb on + strand, within MMP_RS01375 at 256.949 kb on - strand, within MMP_RS01375 at 256.980 kb on - strand, within MMP_RS01375 at 256.983 kb on + strand, within MMP_RS01375 at 256.983 kb on + strand, within MMP_RS01375 at 257.009 kb on - strand, within MMP_RS01375 at 257.010 kb on + strand, within MMP_RS01375 at 257.012 kb on + strand, within MMP_RS01375 at 257.015 kb on - strand, within MMP_RS01375 at 257.040 kb on - strand, within MMP_RS01375 at 257.052 kb on - strand, within MMP_RS01375 at 257.083 kb on - strand, within MMP_RS01375 at 257.090 kb on + strand, within MMP_RS01375 at 257.108 kb on + strand, within MMP_RS01375 at 257.108 kb on + strand, within MMP_RS01375 at 257.110 kb on - strand, within MMP_RS01375 at 257.121 kb on + strand, within MMP_RS01375 at 257.181 kb on + strand, within MMP_RS01375 at 257.182 kb on - strand, within MMP_RS01375 at 257.183 kb on + strand, within MMP_RS01375 at 257.183 kb on + strand, within MMP_RS01375 at 257.209 kb on + strand at 257.251 kb on + strand at 257.354 kb on + strand at 257.354 kb on + strand at 257.358 kb on - strand at 257.395 kb on + strand at 257.415 kb on - strand at 257.444 kb on - strand at 257.619 kb on - strand, within MMP_RS01380 at 257.620 kb on + strand, within MMP_RS01380 at 257.839 kb on + strand, within MMP_RS01380 at 257.842 kb on - strand, within MMP_RS01380 at 258.008 kb on - strand, within MMP_RS01380 at 258.066 kb on + strand, within MMP_RS01380 at 258.121 kb on + strand, within MMP_RS01380 at 258.126 kb on + strand, within MMP_RS01380 at 258.176 kb on - strand, within MMP_RS01380 at 258.220 kb on - strand at 258.223 kb on + strand at 258.309 kb on - strand at 258.314 kb on + strand at 258.314 kb on + strand at 258.865 kb on - strand, within MMP_RS01385 at 258.908 kb on - strand at 258.943 kb on - strand at 258.974 kb on - strand at 258.993 kb on - strand at 258.997 kb on - strand at 258.998 kb on + strand at 258.998 kb on + strand at 259.147 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 256,402 - MMP_RS01375 0.37 +0.1 256,418 - MMP_RS01375 0.38 -0.3 256,419 + MMP_RS01375 0.38 -0.8 256,450 - MMP_RS01375 0.40 -0.4 256,451 + MMP_RS01375 0.40 -0.3 256,455 + MMP_RS01375 0.41 +0.5 256,455 + MMP_RS01375 0.41 +0.3 256,490 - MMP_RS01375 0.43 +0.3 256,492 - MMP_RS01375 0.43 +0.0 256,493 + MMP_RS01375 0.43 -0.1 256,561 + MMP_RS01375 0.48 -0.2 256,796 + MMP_RS01375 0.63 +0.9 256,807 + MMP_RS01375 0.64 -0.8 256,809 + MMP_RS01375 0.64 -0.0 256,809 + MMP_RS01375 0.64 +0.2 256,825 - MMP_RS01375 0.65 -1.0 256,834 - MMP_RS01375 0.65 -0.5 256,874 - MMP_RS01375 0.68 +0.9 256,888 - MMP_RS01375 0.69 +0.2 256,912 - MMP_RS01375 0.71 +1.2 256,921 + MMP_RS01375 0.71 +0.3 256,949 - MMP_RS01375 0.73 +0.6 256,980 - MMP_RS01375 0.75 -0.2 256,983 + MMP_RS01375 0.75 -0.5 256,983 + MMP_RS01375 0.75 -0.1 257,009 - MMP_RS01375 0.77 -0.1 257,010 + MMP_RS01375 0.77 -0.0 257,012 + MMP_RS01375 0.77 +0.7 257,015 - MMP_RS01375 0.77 +0.2 257,040 - MMP_RS01375 0.79 -0.5 257,052 - MMP_RS01375 0.80 -0.4 257,083 - MMP_RS01375 0.82 +0.1 257,090 + MMP_RS01375 0.82 +0.3 257,108 + MMP_RS01375 0.83 +0.1 257,108 + MMP_RS01375 0.83 -0.2 257,110 - MMP_RS01375 0.84 -1.3 257,121 + MMP_RS01375 0.84 +0.2 257,181 + MMP_RS01375 0.88 -0.3 257,182 - MMP_RS01375 0.88 -0.2 257,183 + MMP_RS01375 0.88 +0.1 257,183 + MMP_RS01375 0.88 +0.0 257,209 + -0.8 257,251 + +0.3 257,354 + -0.4 257,354 + -0.2 257,358 - -0.3 257,395 + -1.4 257,415 - -4.5 257,444 - -2.9 257,619 - MMP_RS01380 0.26 -2.1 257,620 + MMP_RS01380 0.27 -3.5 257,839 + MMP_RS01380 0.51 -2.7 257,842 - MMP_RS01380 0.51 -3.9 258,008 - MMP_RS01380 0.70 -1.7 258,066 + MMP_RS01380 0.76 -1.8 258,121 + MMP_RS01380 0.82 -2.6 258,126 + MMP_RS01380 0.83 -2.4 258,176 - MMP_RS01380 0.88 -2.2 258,220 - -2.5 258,223 + -4.0 258,309 - +0.4 258,314 + +0.5 258,314 + -0.6 258,865 - MMP_RS01385 0.90 -2.2 258,908 - -2.5 258,943 - -1.3 258,974 - -1.1 258,993 - -1.9 258,997 - -2.0 258,998 + -0.6 258,998 + -2.9 259,147 + -2.6
Or see this region's nucleotide sequence