Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01040 and MMP_RS01045 are separated by 89 nucleotides MMP_RS01045 and MMP_RS01050 are separated by 84 nucleotides MMP_RS01050 and MMP_RS01055 are separated by 334 nucleotides
MMP_RS01040: MMP_RS01040 - phosphomethylpyrimidine synthase, at 195,229 to 196,509
_RS01040
MMP_RS01045: MMP_RS01045 - diphthamide biosynthesis enzyme Dph2, at 196,599 to 197,528
_RS01045
MMP_RS01050: MMP_RS01050 - tRNA-Leu, at 197,613 to 197,697
_RS01050
MMP_RS01055: MMP_RS01055 - hypothetical protein, at 198,032 to 198,409
_RS01055
Position (kb) 196
197
198 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 196.329 kb on - strand at 196.330 kb on + strand at 196.540 kb on - strand at 196.542 kb on - strand at 196.554 kb on - strand at 196.558 kb on + strand at 196.560 kb on - strand at 196.560 kb on - strand at 196.560 kb on - strand at 196.564 kb on - strand at 196.564 kb on - strand at 196.565 kb on + strand at 196.565 kb on + strand at 196.599 kb on + strand at 196.781 kb on + strand, within MMP_RS01045 at 196.817 kb on + strand, within MMP_RS01045 at 196.892 kb on - strand, within MMP_RS01045 at 196.959 kb on + strand, within MMP_RS01045 at 196.998 kb on + strand, within MMP_RS01045 at 196.998 kb on + strand, within MMP_RS01045 at 197.096 kb on - strand, within MMP_RS01045 at 197.209 kb on - strand, within MMP_RS01045 at 197.209 kb on - strand, within MMP_RS01045 at 197.322 kb on - strand, within MMP_RS01045 at 197.323 kb on + strand, within MMP_RS01045 at 197.325 kb on + strand, within MMP_RS01045 at 197.443 kb on - strand at 197.443 kb on - strand at 197.470 kb on + strand at 197.516 kb on - strand at 197.524 kb on + strand at 197.811 kb on - strand at 197.856 kb on - strand at 197.894 kb on - strand at 198.086 kb on - strand, within MMP_RS01055 at 198.086 kb on - strand, within MMP_RS01055 at 198.170 kb on + strand, within MMP_RS01055 at 198.406 kb on + strand at 198.406 kb on + strand at 198.411 kb on - strand at 198.411 kb on - strand at 198.412 kb on + strand at 198.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 196,329 - -2.9 196,330 + -2.0 196,540 - -0.9 196,542 - -0.6 196,554 - -0.6 196,558 + -1.4 196,560 - -0.5 196,560 - -2.0 196,560 - -0.7 196,564 - -0.5 196,564 - +0.2 196,565 + -2.0 196,565 + -0.7 196,599 + -1.2 196,781 + MMP_RS01045 0.20 -2.0 196,817 + MMP_RS01045 0.23 -0.8 196,892 - MMP_RS01045 0.32 -2.4 196,959 + MMP_RS01045 0.39 -0.9 196,998 + MMP_RS01045 0.43 -0.7 196,998 + MMP_RS01045 0.43 -0.9 197,096 - MMP_RS01045 0.53 -0.8 197,209 - MMP_RS01045 0.66 -1.3 197,209 - MMP_RS01045 0.66 -1.7 197,322 - MMP_RS01045 0.78 -1.8 197,323 + MMP_RS01045 0.78 -1.0 197,325 + MMP_RS01045 0.78 -1.2 197,443 - -2.2 197,443 - -1.2 197,470 + -0.5 197,516 - +0.1 197,524 + +0.1 197,811 - +0.8 197,856 - +1.2 197,894 - -0.3 198,086 - MMP_RS01055 0.14 +0.5 198,086 - MMP_RS01055 0.14 +0.4 198,170 + MMP_RS01055 0.37 +0.9 198,406 + -0.6 198,406 + +0.1 198,411 - +0.1 198,411 - -0.5 198,412 + +1.2 198,444 - -0.3
Or see this region's nucleotide sequence