Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00730 and MMP_RS00735 are separated by 59 nucleotides MMP_RS00735 and MMP_RS00740 are separated by 306 nucleotides MMP_RS00740 and MMP_RS00745 are separated by 77 nucleotides
MMP_RS00730: MMP_RS00730 - 5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB, at 132,540 to 133,565
_RS00730
MMP_RS00735: MMP_RS00735 - 5 10-methenyltetrahydromethanopterin hydrogenase, at 133,625 to 134,689
_RS00735
MMP_RS00740: MMP_RS00740 - cyclase family protein, at 134,996 to 135,565
_RS00740
MMP_RS00745: MMP_RS00745 - histidinol phosphate phosphatase domain-containing protein, at 135,643 to 136,299
_RS00745
Position (kb) 133
134
135 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 132.899 kb on - strand, within MMP_RS00730 at 132.942 kb on - strand, within MMP_RS00730 at 133.070 kb on - strand, within MMP_RS00730 at 133.104 kb on - strand, within MMP_RS00730 at 133.174 kb on + strand, within MMP_RS00730 at 133.288 kb on + strand, within MMP_RS00730 at 133.362 kb on - strand, within MMP_RS00730 at 133.441 kb on - strand, within MMP_RS00730 at 133.490 kb on - strand at 133.586 kb on - strand at 133.609 kb on - strand at 133.622 kb on - strand at 133.713 kb on + strand at 134.255 kb on - strand, within MMP_RS00735 at 134.255 kb on - strand, within MMP_RS00735 at 134.424 kb on + strand, within MMP_RS00735 at 134.713 kb on - strand at 134.713 kb on - strand at 134.723 kb on - strand at 134.757 kb on + strand at 134.760 kb on - strand at 134.760 kb on - strand at 134.782 kb on + strand at 134.863 kb on - strand at 134.900 kb on + strand at 134.982 kb on + strand at 135.287 kb on - strand, within MMP_RS00740 at 135.338 kb on + strand, within MMP_RS00740 at 135.421 kb on - strand, within MMP_RS00740 at 135.422 kb on + strand, within MMP_RS00740 at 135.565 kb on - strand at 135.600 kb on + strand at 135.606 kb on + strand at 135.613 kb on - strand at 135.613 kb on - strand at 135.619 kb on + strand at 135.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 132,899 - MMP_RS00730 0.35 +0.5 132,942 - MMP_RS00730 0.39 -0.2 133,070 - MMP_RS00730 0.52 -1.8 133,104 - MMP_RS00730 0.55 -3.9 133,174 + MMP_RS00730 0.62 +0.3 133,288 + MMP_RS00730 0.73 -1.7 133,362 - MMP_RS00730 0.80 -2.0 133,441 - MMP_RS00730 0.88 -0.9 133,490 - +0.2 133,586 - -0.6 133,609 - -1.0 133,622 - -1.5 133,713 + -2.5 134,255 - MMP_RS00735 0.59 -1.3 134,255 - MMP_RS00735 0.59 -1.4 134,424 + MMP_RS00735 0.75 -1.9 134,713 - -1.2 134,713 - +0.6 134,723 - -1.1 134,757 + -2.7 134,760 - +0.6 134,760 - -1.3 134,782 + -0.0 134,863 - +0.1 134,900 + +0.3 134,982 + +0.3 135,287 - MMP_RS00740 0.51 +0.7 135,338 + MMP_RS00740 0.60 +0.1 135,421 - MMP_RS00740 0.75 -0.1 135,422 + MMP_RS00740 0.75 -0.3 135,565 - +0.5 135,600 + -0.4 135,606 + -0.8 135,613 - -1.1 135,613 - -2.4 135,619 + -1.2 135,675 - -2.9
Or see this region's nucleotide sequence