Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18890 and BWI76_RS18895 are separated by 164 nucleotides BWI76_RS18895 and BWI76_RS18900 overlap by 1 nucleotides BWI76_RS18900 and BWI76_RS18905 are separated by 74 nucleotides
BWI76_RS18890: BWI76_RS18890 - nif-specific transcriptional activator NifA, at 3,917,105 to 3,918,679
_RS18890
BWI76_RS18895: BWI76_RS18895 - nitrogenase cofactor biosynthesis protein NifB, at 3,918,844 to 3,920,250
_RS18895
BWI76_RS18900: BWI76_RS18900 - protein NifQ, at 3,920,250 to 3,920,753
_RS18900
BWI76_RS18905: BWI76_RS18905 - putrescine/spermidine ABC transporter, at 3,920,828 to 3,922,186
_RS18905
Position (kb)
3918
3919
3920
3921 Strain fitness (log2 ratio)
-2
-1
0
1 at 3917.874 kb on - strand, within BWI76_RS18890 at 3918.008 kb on - strand, within BWI76_RS18890 at 3918.214 kb on - strand, within BWI76_RS18890 at 3918.537 kb on + strand at 3918.592 kb on + strand at 3918.600 kb on - strand at 3918.820 kb on - strand at 3918.828 kb on + strand at 3918.879 kb on - strand at 3919.053 kb on + strand, within BWI76_RS18895 at 3919.076 kb on - strand, within BWI76_RS18895 at 3919.088 kb on - strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.431 kb on + strand, within BWI76_RS18895 at 3919.431 kb on + strand, within BWI76_RS18895 at 3919.550 kb on - strand, within BWI76_RS18895 at 3920.014 kb on - strand, within BWI76_RS18895 at 3920.081 kb on - strand, within BWI76_RS18895 at 3920.334 kb on - strand, within BWI76_RS18900 at 3920.451 kb on + strand, within BWI76_RS18900 at 3920.565 kb on - strand, within BWI76_RS18900 at 3920.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube remove 3,917,874 - BWI76_RS18890 0.49 -0.8 3,918,008 - BWI76_RS18890 0.57 -1.4 3,918,214 - BWI76_RS18890 0.70 -0.7 3,918,537 + -1.4 3,918,592 + -0.1 3,918,600 - -1.6 3,918,820 - -0.6 3,918,828 + -1.2 3,918,879 - -0.1 3,919,053 + BWI76_RS18895 0.15 -1.1 3,919,076 - BWI76_RS18895 0.16 -2.0 3,919,088 - BWI76_RS18895 0.17 -0.1 3,919,202 + BWI76_RS18895 0.25 -2.3 3,919,202 + BWI76_RS18895 0.25 -1.6 3,919,202 + BWI76_RS18895 0.25 -2.2 3,919,431 + BWI76_RS18895 0.42 -1.4 3,919,431 + BWI76_RS18895 0.42 -2.3 3,919,550 - BWI76_RS18895 0.50 -1.3 3,920,014 - BWI76_RS18895 0.83 -1.6 3,920,081 - BWI76_RS18895 0.88 -1.7 3,920,334 - BWI76_RS18900 0.17 -1.0 3,920,451 + BWI76_RS18900 0.40 -1.7 3,920,565 - BWI76_RS18900 0.62 -0.8 3,920,846 - +0.6
Or see this region's nucleotide sequence