Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18825 and BWI76_RS18830 are separated by 10 nucleotides BWI76_RS18830 and BWI76_RS18835 are separated by 322 nucleotides BWI76_RS18835 and BWI76_RS18840 are separated by 10 nucleotides
BWI76_RS18825: BWI76_RS18825 - protein NifT, at 3,905,395 to 3,905,613
_RS18825
BWI76_RS18830: BWI76_RS18830 - protein NifY, at 3,905,624 to 3,906,286
_RS18830
BWI76_RS18835: BWI76_RS18835 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,906,609 to 3,907,982
_RS18835
BWI76_RS18840: BWI76_RS18840 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 3,907,993 to 3,909,378
_RS18840
Position (kb)
3906
3907
3908 Strain fitness (log2 ratio)
-2
-1
0
1 at 3905.675 kb on + strand at 3905.848 kb on - strand, within BWI76_RS18830 at 3905.885 kb on + strand, within BWI76_RS18830 at 3906.358 kb on + strand at 3906.405 kb on + strand at 3906.470 kb on - strand at 3906.540 kb on + strand at 3906.749 kb on + strand, within BWI76_RS18835 at 3906.881 kb on + strand, within BWI76_RS18835 at 3906.922 kb on - strand, within BWI76_RS18835 at 3907.024 kb on - strand, within BWI76_RS18835 at 3907.049 kb on + strand, within BWI76_RS18835 at 3907.394 kb on - strand, within BWI76_RS18835 at 3907.405 kb on - strand, within BWI76_RS18835 at 3907.984 kb on - strand at 3908.111 kb on - strand at 3908.286 kb on + strand, within BWI76_RS18840 at 3908.294 kb on - strand, within BWI76_RS18840 at 3908.372 kb on - strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.660 kb on - strand, within BWI76_RS18840 at 3908.681 kb on - strand, within BWI76_RS18840 at 3908.698 kb on + strand, within BWI76_RS18840 at 3908.706 kb on - strand, within BWI76_RS18840 at 3908.716 kb on - strand, within BWI76_RS18840 at 3908.798 kb on + strand, within BWI76_RS18840 at 3908.909 kb on + strand, within BWI76_RS18840 at 3908.917 kb on - strand, within BWI76_RS18840 at 3908.981 kb on - strand, within BWI76_RS18840
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube remove 3,905,675 + -0.8 3,905,848 - BWI76_RS18830 0.34 -0.5 3,905,885 + BWI76_RS18830 0.39 -0.8 3,906,358 + +0.2 3,906,405 + -0.3 3,906,470 - +0.3 3,906,540 + -2.2 3,906,749 + BWI76_RS18835 0.10 -1.2 3,906,881 + BWI76_RS18835 0.20 -1.7 3,906,922 - BWI76_RS18835 0.23 -1.7 3,907,024 - BWI76_RS18835 0.30 -2.4 3,907,049 + BWI76_RS18835 0.32 -1.9 3,907,394 - BWI76_RS18835 0.57 -0.9 3,907,405 - BWI76_RS18835 0.58 -0.5 3,907,984 - -2.2 3,908,111 - -0.2 3,908,286 + BWI76_RS18840 0.21 -1.5 3,908,294 - BWI76_RS18840 0.22 -1.6 3,908,372 - BWI76_RS18840 0.27 +0.0 3,908,437 + BWI76_RS18840 0.32 -1.2 3,908,437 + BWI76_RS18840 0.32 -0.4 3,908,437 + BWI76_RS18840 0.32 -0.4 3,908,445 - BWI76_RS18840 0.33 -2.3 3,908,445 - BWI76_RS18840 0.33 -1.4 3,908,445 - BWI76_RS18840 0.33 -1.2 3,908,445 - BWI76_RS18840 0.33 -1.8 3,908,445 - BWI76_RS18840 0.33 -1.6 3,908,660 - BWI76_RS18840 0.48 -1.5 3,908,681 - BWI76_RS18840 0.50 -0.9 3,908,698 + BWI76_RS18840 0.51 -1.7 3,908,706 - BWI76_RS18840 0.51 -1.3 3,908,716 - BWI76_RS18840 0.52 -2.2 3,908,798 + BWI76_RS18840 0.58 -0.8 3,908,909 + BWI76_RS18840 0.66 -0.4 3,908,917 - BWI76_RS18840 0.67 -0.8 3,908,981 - BWI76_RS18840 0.71 -1.2
Or see this region's nucleotide sequence