Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18805

Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18790 and BWI76_RS18795 are separated by 42 nucleotidesBWI76_RS18795 and BWI76_RS18800 are separated by 1 nucleotidesBWI76_RS18800 and BWI76_RS18805 are separated by 7 nucleotidesBWI76_RS18805 and BWI76_RS18810 are separated by 404 nucleotides BWI76_RS18790: BWI76_RS18790 - elongation factor G, at 3,894,811 to 3,896,913 _RS18790 BWI76_RS18795: BWI76_RS18795 - hypothetical protein, at 3,896,956 to 3,897,150 _RS18795 BWI76_RS18800: BWI76_RS18800 - transcriptional regulator, at 3,897,152 to 3,897,463 _RS18800 BWI76_RS18805: BWI76_RS18805 - pyruvate:ferredoxin (flavodoxin) oxidoreductase, at 3,897,471 to 3,900,986 _RS18805 BWI76_RS18810: BWI76_RS18810 - nitrogenase iron protein, at 3,901,391 to 3,902,272 _RS18810 Position (kb) 3897 3898 3899 3900 3901Strain fitness (log2 ratio) -2 -1 0 1at 3896.648 kb on - strand, within BWI76_RS18790at 3896.658 kb on + strand, within BWI76_RS18790at 3897.135 kb on - strandat 3897.268 kb on + strand, within BWI76_RS18800at 3898.790 kb on + strand, within BWI76_RS18805at 3898.859 kb on + strand, within BWI76_RS18805at 3898.908 kb on - strand, within BWI76_RS18805at 3899.166 kb on - strand, within BWI76_RS18805at 3899.237 kb on + strand, within BWI76_RS18805at 3899.479 kb on - strand, within BWI76_RS18805at 3899.479 kb on - strand, within BWI76_RS18805at 3899.511 kb on - strand, within BWI76_RS18805at 3900.131 kb on - strand, within BWI76_RS18805at 3900.193 kb on - strand, within BWI76_RS18805at 3900.286 kb on - strand, within BWI76_RS18805at 3900.319 kb on + strand, within BWI76_RS18805at 3900.424 kb on + strand, within BWI76_RS18805at 3900.495 kb on - strand, within BWI76_RS18805at 3901.153 kb on + strandat 3901.185 kb on - strandat 3901.401 kb on - strandat 3901.456 kb on - strandat 3901.456 kb on - strandat 3901.791 kb on - strand, within BWI76_RS18810

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube
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3,896,648 - BWI76_RS18790 0.87 +0.2
3,896,658 + BWI76_RS18790 0.88 -0.1
3,897,135 - -0.9
3,897,268 + BWI76_RS18800 0.37 -0.4
3,898,790 + BWI76_RS18805 0.38 -1.1
3,898,859 + BWI76_RS18805 0.39 -1.9
3,898,908 - BWI76_RS18805 0.41 -1.4
3,899,166 - BWI76_RS18805 0.48 -1.3
3,899,237 + BWI76_RS18805 0.50 -0.5
3,899,479 - BWI76_RS18805 0.57 -1.7
3,899,479 - BWI76_RS18805 0.57 +0.0
3,899,511 - BWI76_RS18805 0.58 -0.8
3,900,131 - BWI76_RS18805 0.76 -0.5
3,900,193 - BWI76_RS18805 0.77 -1.9
3,900,286 - BWI76_RS18805 0.80 -0.8
3,900,319 + BWI76_RS18805 0.81 -0.2
3,900,424 + BWI76_RS18805 0.84 -2.2
3,900,495 - BWI76_RS18805 0.86 -1.4
3,901,153 + -0.8
3,901,185 - -1.2
3,901,401 - +0.0
3,901,456 - -2.6
3,901,456 - +0.2
3,901,791 - BWI76_RS18810 0.45 -0.1

Or see this region's nucleotide sequence