Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12925 and BWI76_RS12930 overlap by 4 nucleotides BWI76_RS12930 and BWI76_RS12935 overlap by 11 nucleotides BWI76_RS12935 and BWI76_RS12940 are separated by 77 nucleotides BWI76_RS12940 and BWI76_RS12945 are separated by 57 nucleotides
BWI76_RS12925: BWI76_RS12925 - efflux transporter periplasmic adaptor subunit, at 2,692,794 to 2,693,717
_RS12925
BWI76_RS12930: BWI76_RS12930 - DUF1656 domain-containing protein, at 2,693,714 to 2,693,929
_RS12930
BWI76_RS12935: BWI76_RS12935 - fusaric acid resistance protein, at 2,693,919 to 2,695,958
_RS12935
BWI76_RS12940: BWI76_RS12940 - AraC family transcriptional regulator, at 2,696,036 to 2,696,857
_RS12940
BWI76_RS12945: BWI76_RS12945 - AraC family transcriptional regulator, at 2,696,915 to 2,697,310
_RS12945
Position (kb)
2693
2694
2695
2696 Strain fitness (log2 ratio)
-2
-1
0
1 at 2692.996 kb on - strand, within BWI76_RS12925 at 2693.465 kb on + strand, within BWI76_RS12925 at 2693.734 kb on - strand at 2694.051 kb on + strand at 2694.369 kb on - strand, within BWI76_RS12935 at 2694.511 kb on - strand, within BWI76_RS12935 at 2694.527 kb on + strand, within BWI76_RS12935 at 2694.980 kb on + strand, within BWI76_RS12935 at 2695.368 kb on - strand, within BWI76_RS12935 at 2695.368 kb on - strand, within BWI76_RS12935 at 2695.485 kb on + strand, within BWI76_RS12935 at 2695.493 kb on - strand, within BWI76_RS12935 at 2695.854 kb on + strand at 2695.885 kb on + strand at 2695.885 kb on + strand at 2696.060 kb on - strand at 2696.181 kb on + strand, within BWI76_RS12940 at 2696.414 kb on + strand, within BWI76_RS12940 at 2696.777 kb on - strand at 2696.859 kb on - strand at 2696.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube remove 2,692,996 - BWI76_RS12925 0.22 -0.4 2,693,465 + BWI76_RS12925 0.73 -0.1 2,693,734 - +0.0 2,694,051 + -0.2 2,694,369 - BWI76_RS12935 0.22 +0.3 2,694,511 - BWI76_RS12935 0.29 -2.5 2,694,527 + BWI76_RS12935 0.30 -0.5 2,694,980 + BWI76_RS12935 0.52 -0.4 2,695,368 - BWI76_RS12935 0.71 -0.4 2,695,368 - BWI76_RS12935 0.71 -0.4 2,695,485 + BWI76_RS12935 0.77 -0.3 2,695,493 - BWI76_RS12935 0.77 -0.4 2,695,854 + +0.6 2,695,885 + +0.5 2,695,885 + +0.2 2,696,060 - -0.1 2,696,181 + BWI76_RS12940 0.18 +0.3 2,696,414 + BWI76_RS12940 0.46 -0.0 2,696,777 - -0.7 2,696,859 - -0.2 2,696,859 - +0.2
Or see this region's nucleotide sequence