Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00100

Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00095 and BWI76_RS00100 are separated by 147 nucleotidesBWI76_RS00100 and BWI76_RS00105 are separated by 37 nucleotidesBWI76_RS00105 and BWI76_RS00110 are separated by 48 nucleotides BWI76_RS00095: BWI76_RS00095 - DNA-binding transcriptional regulator, at 18,362 to 19,321 _RS00095 BWI76_RS00100: BWI76_RS00100 - hypothetical protein, at 19,469 to 20,206 _RS00100 BWI76_RS00105: BWI76_RS00105 - NAD(P)H-dependent oxidoreductase, at 20,244 to 20,810 _RS00105 BWI76_RS00110: BWI76_RS00110 - adenine permease PurP, at 20,859 to 22,199 _RS00110 Position (kb) 19 20 21Strain fitness (log2 ratio) -2 -1 0 1 2at 18.544 kb on - strandat 18.570 kb on + strand, within BWI76_RS00095at 18.904 kb on + strand, within BWI76_RS00095at 19.096 kb on + strand, within BWI76_RS00095at 19.227 kb on + strandat 19.300 kb on + strandat 19.308 kb on - strandat 19.308 kb on - strandat 19.313 kb on - strandat 19.623 kb on - strand, within BWI76_RS00100at 19.625 kb on - strand, within BWI76_RS00100at 19.967 kb on + strand, within BWI76_RS00100at 20.030 kb on + strand, within BWI76_RS00100at 20.038 kb on - strand, within BWI76_RS00100at 20.053 kb on - strand, within BWI76_RS00100at 20.123 kb on + strand, within BWI76_RS00100at 20.244 kb on - strandat 20.281 kb on - strandat 20.314 kb on + strand, within BWI76_RS00105at 20.322 kb on - strand, within BWI76_RS00105at 20.350 kb on - strand, within BWI76_RS00105at 20.436 kb on - strand, within BWI76_RS00105at 20.467 kb on - strand, within BWI76_RS00105at 20.495 kb on - strand, within BWI76_RS00105at 20.502 kb on + strand, within BWI76_RS00105at 20.588 kb on - strand, within BWI76_RS00105at 20.715 kb on - strand, within BWI76_RS00105at 20.806 kb on - strandat 20.835 kb on - strandat 20.856 kb on + strandat 20.856 kb on + strandat 20.864 kb on - strandat 20.929 kb on - strandat 20.929 kb on - strandat 20.989 kb on - strandat 21.080 kb on + strand, within BWI76_RS00110

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube
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18,544 - +2.6
18,570 + BWI76_RS00095 0.22 +0.2
18,904 + BWI76_RS00095 0.56 +0.2
19,096 + BWI76_RS00095 0.76 +0.9
19,227 + -0.7
19,300 + -1.0
19,308 - +1.3
19,308 - +0.6
19,313 - -0.3
19,623 - BWI76_RS00100 0.21 -0.9
19,625 - BWI76_RS00100 0.21 -0.2
19,967 + BWI76_RS00100 0.67 -2.3
20,030 + BWI76_RS00100 0.76 +0.3
20,038 - BWI76_RS00100 0.77 -0.5
20,053 - BWI76_RS00100 0.79 -2.2
20,123 + BWI76_RS00100 0.89 +0.1
20,244 - -0.1
20,281 - -0.6
20,314 + BWI76_RS00105 0.12 +0.4
20,322 - BWI76_RS00105 0.14 -0.4
20,350 - BWI76_RS00105 0.19 +1.0
20,436 - BWI76_RS00105 0.34 +0.4
20,467 - BWI76_RS00105 0.39 -0.2
20,495 - BWI76_RS00105 0.44 -0.0
20,502 + BWI76_RS00105 0.46 -1.5
20,588 - BWI76_RS00105 0.61 +0.3
20,715 - BWI76_RS00105 0.83 -2.3
20,806 - -0.3
20,835 - +0.4
20,856 + -0.0
20,856 + +0.1
20,864 - -0.5
20,929 - +1.6
20,929 - -0.3
20,989 - +0.0
21,080 + BWI76_RS00110 0.16 -1.0

Or see this region's nucleotide sequence