Strain Fitness in Methanococcus maripaludis S2 around MMP_RS08715

Experiment: H2 McCas with 10 mM polysulfide

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08705 and MMP_RS08710 are separated by 1 nucleotidesMMP_RS08710 and MMP_RS08715 are separated by 212 nucleotidesMMP_RS08715 and MMP_RS08720 are separated by 29 nucleotides MMP_RS08705: MMP_RS08705 - sulfopyruvate decarboxylase subunit beta, at 1,629,931 to 1,630,488 _RS08705 MMP_RS08710: MMP_RS08710 - HisA/HisF family protein, at 1,630,490 to 1,631,158 _RS08710 MMP_RS08715: MMP_RS08715 - formylmethanofuran dehydrogenase subunit B, at 1,631,371 to 1,632,675 _RS08715 MMP_RS08720: MMP_RS08720 - 4Fe-4S binding protein, at 1,632,705 to 1,633,892 _RS08720 Position (kb) 1631 1632 1633Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1630.419 kb on - strand, within MMP_RS08705at 1630.420 kb on + strand, within MMP_RS08705at 1630.488 kb on - strandat 1630.492 kb on + strandat 1630.521 kb on + strandat 1630.609 kb on - strandat 1630.610 kb on + strandat 1630.626 kb on - strandat 1630.626 kb on - strandat 1630.627 kb on + strandat 1630.627 kb on + strandat 1630.627 kb on + strandat 1630.652 kb on - strandat 1630.655 kb on + strandat 1630.661 kb on - strandat 1630.997 kb on + strandat 1630.999 kb on + strandat 1631.132 kb on - strandat 1631.149 kb on - strandat 1631.168 kb on - strandat 1631.180 kb on + strandat 1631.215 kb on - strandat 1631.216 kb on + strandat 1631.216 kb on + strandat 1631.265 kb on + strandat 1631.282 kb on - strandat 1631.287 kb on - strandat 1631.579 kb on + strand, within MMP_RS08715at 1631.827 kb on + strand, within MMP_RS08715at 1631.829 kb on - strand, within MMP_RS08715at 1632.471 kb on + strand, within MMP_RS08715at 1632.652 kb on - strandat 1632.782 kb on - strandat 1633.411 kb on + strand, within MMP_RS08720at 1633.412 kb on - strand, within MMP_RS08720at 1633.668 kb on - strand, within MMP_RS08720

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide
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1,630,419 - MMP_RS08705 0.87 -0.9
1,630,420 + MMP_RS08705 0.88 +0.2
1,630,488 - -0.2
1,630,492 + +0.2
1,630,521 + +0.4
1,630,609 - -1.5
1,630,610 + -2.4
1,630,626 - -3.4
1,630,626 - -2.5
1,630,627 + -1.7
1,630,627 + -2.6
1,630,627 + -0.9
1,630,652 - -2.8
1,630,655 + -1.4
1,630,661 - -3.3
1,630,997 + -4.7
1,630,999 + -2.2
1,631,132 - -3.1
1,631,149 - -2.2
1,631,168 - +0.6
1,631,180 + +0.0
1,631,215 - +0.3
1,631,216 + +0.6
1,631,216 + +0.7
1,631,265 + +0.2
1,631,282 - +0.1
1,631,287 - +0.4
1,631,579 + MMP_RS08715 0.16 -2.5
1,631,827 + MMP_RS08715 0.35 -2.0
1,631,829 - MMP_RS08715 0.35 -2.8
1,632,471 + MMP_RS08715 0.84 -2.3
1,632,652 - -2.9
1,632,782 - -3.4
1,633,411 + MMP_RS08720 0.59 -1.4
1,633,412 - MMP_RS08720 0.60 -2.1
1,633,668 - MMP_RS08720 0.81 -2.7

Or see this region's nucleotide sequence