Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08705 and MMP_RS08710 are separated by 1 nucleotides MMP_RS08710 and MMP_RS08715 are separated by 212 nucleotides MMP_RS08715 and MMP_RS08720 are separated by 29 nucleotides
MMP_RS08705: MMP_RS08705 - sulfopyruvate decarboxylase subunit beta, at 1,629,931 to 1,630,488
_RS08705
MMP_RS08710: MMP_RS08710 - HisA/HisF family protein, at 1,630,490 to 1,631,158
_RS08710
MMP_RS08715: MMP_RS08715 - formylmethanofuran dehydrogenase subunit B, at 1,631,371 to 1,632,675
_RS08715
MMP_RS08720: MMP_RS08720 - 4Fe-4S binding protein, at 1,632,705 to 1,633,892
_RS08720
Position (kb)
1631
1632
1633 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1630.419 kb on - strand, within MMP_RS08705 at 1630.420 kb on + strand, within MMP_RS08705 at 1630.488 kb on - strand at 1630.492 kb on + strand at 1630.521 kb on + strand at 1630.609 kb on - strand at 1630.610 kb on + strand at 1630.626 kb on - strand at 1630.626 kb on - strand at 1630.627 kb on + strand at 1630.627 kb on + strand at 1630.627 kb on + strand at 1630.652 kb on - strand at 1630.655 kb on + strand at 1630.661 kb on - strand at 1630.997 kb on + strand at 1630.999 kb on + strand at 1631.132 kb on - strand at 1631.149 kb on - strand at 1631.168 kb on - strand at 1631.180 kb on + strand at 1631.215 kb on - strand at 1631.216 kb on + strand at 1631.216 kb on + strand at 1631.265 kb on + strand at 1631.282 kb on - strand at 1631.287 kb on - strand at 1631.579 kb on + strand, within MMP_RS08715 at 1631.827 kb on + strand, within MMP_RS08715 at 1631.829 kb on - strand, within MMP_RS08715 at 1632.471 kb on + strand, within MMP_RS08715 at 1632.652 kb on - strand at 1632.782 kb on - strand at 1633.411 kb on + strand, within MMP_RS08720 at 1633.412 kb on - strand, within MMP_RS08720 at 1633.668 kb on - strand, within MMP_RS08720
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 1,630,419 - MMP_RS08705 0.87 -0.9 1,630,420 + MMP_RS08705 0.88 +0.2 1,630,488 - -0.2 1,630,492 + +0.2 1,630,521 + +0.4 1,630,609 - -1.5 1,630,610 + -2.4 1,630,626 - -3.4 1,630,626 - -2.5 1,630,627 + -1.7 1,630,627 + -2.6 1,630,627 + -0.9 1,630,652 - -2.8 1,630,655 + -1.4 1,630,661 - -3.3 1,630,997 + -4.7 1,630,999 + -2.2 1,631,132 - -3.1 1,631,149 - -2.2 1,631,168 - +0.6 1,631,180 + +0.0 1,631,215 - +0.3 1,631,216 + +0.6 1,631,216 + +0.7 1,631,265 + +0.2 1,631,282 - +0.1 1,631,287 - +0.4 1,631,579 + MMP_RS08715 0.16 -2.5 1,631,827 + MMP_RS08715 0.35 -2.0 1,631,829 - MMP_RS08715 0.35 -2.8 1,632,471 + MMP_RS08715 0.84 -2.3 1,632,652 - -2.9 1,632,782 - -3.4 1,633,411 + MMP_RS08720 0.59 -1.4 1,633,412 - MMP_RS08720 0.60 -2.1 1,633,668 - MMP_RS08720 0.81 -2.7
Or see this region's nucleotide sequence