Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06725 and MMP_RS06730 are separated by 25 nucleotides MMP_RS06730 and MMP_RS06735 are separated by 62 nucleotides
MMP_RS06725: MMP_RS06725 - hypothetical protein, at 1,291,237 to 1,292,640
_RS06725
MMP_RS06730: MMP_RS06730 - phosphopantothenate/pantothenate synthetase, at 1,292,666 to 1,293,427
_RS06730
MMP_RS06735: MMP_RS06735 - class I SAM-dependent rRNA methyltransferase, at 1,293,490 to 1,294,632
_RS06735
Position (kb)
1292
1293
1294 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1291.687 kb on + strand, within MMP_RS06725 at 1291.756 kb on + strand, within MMP_RS06725 at 1291.756 kb on + strand, within MMP_RS06725 at 1291.756 kb on + strand, within MMP_RS06725 at 1291.759 kb on - strand, within MMP_RS06725 at 1291.759 kb on - strand, within MMP_RS06725 at 1291.789 kb on + strand, within MMP_RS06725 at 1291.789 kb on + strand, within MMP_RS06725 at 1291.791 kb on - strand, within MMP_RS06725 at 1291.792 kb on + strand, within MMP_RS06725 at 1291.821 kb on + strand, within MMP_RS06725 at 1291.837 kb on - strand, within MMP_RS06725 at 1291.847 kb on + strand, within MMP_RS06725 at 1291.852 kb on - strand, within MMP_RS06725 at 1291.882 kb on + strand, within MMP_RS06725 at 1291.938 kb on + strand, within MMP_RS06725 at 1291.965 kb on - strand, within MMP_RS06725 at 1291.965 kb on - strand, within MMP_RS06725 at 1292.009 kb on - strand, within MMP_RS06725 at 1292.031 kb on + strand, within MMP_RS06725 at 1292.175 kb on - strand, within MMP_RS06725 at 1292.233 kb on + strand, within MMP_RS06725 at 1292.357 kb on + strand, within MMP_RS06725 at 1292.390 kb on - strand, within MMP_RS06725 at 1292.793 kb on + strand, within MMP_RS06730 at 1293.000 kb on + strand, within MMP_RS06730 at 1293.067 kb on - strand, within MMP_RS06730 at 1293.118 kb on + strand, within MMP_RS06730 at 1293.368 kb on - strand at 1293.399 kb on - strand at 1293.438 kb on - strand at 1293.492 kb on + strand at 1293.496 kb on - strand at 1293.525 kb on + strand at 1293.525 kb on + strand at 1293.553 kb on - strand at 1293.553 kb on - strand at 1293.554 kb on + strand at 1293.633 kb on - strand, within MMP_RS06735 at 1293.856 kb on + strand, within MMP_RS06735 at 1294.295 kb on - strand, within MMP_RS06735 at 1294.300 kb on + strand, within MMP_RS06735 at 1294.308 kb on - strand, within MMP_RS06735
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 1,291,687 + MMP_RS06725 0.32 -1.7 1,291,756 + MMP_RS06725 0.37 -2.7 1,291,756 + MMP_RS06725 0.37 -2.8 1,291,756 + MMP_RS06725 0.37 -3.0 1,291,759 - MMP_RS06725 0.37 -2.7 1,291,759 - MMP_RS06725 0.37 -1.5 1,291,789 + MMP_RS06725 0.39 -1.7 1,291,789 + MMP_RS06725 0.39 -2.6 1,291,791 - MMP_RS06725 0.39 -2.3 1,291,792 + MMP_RS06725 0.40 -3.1 1,291,821 + MMP_RS06725 0.42 -2.4 1,291,837 - MMP_RS06725 0.43 -3.1 1,291,847 + MMP_RS06725 0.43 -0.9 1,291,852 - MMP_RS06725 0.44 -1.4 1,291,882 + MMP_RS06725 0.46 -2.4 1,291,938 + MMP_RS06725 0.50 -2.1 1,291,965 - MMP_RS06725 0.52 -4.5 1,291,965 - MMP_RS06725 0.52 -1.5 1,292,009 - MMP_RS06725 0.55 -3.6 1,292,031 + MMP_RS06725 0.57 -2.5 1,292,175 - MMP_RS06725 0.67 -2.8 1,292,233 + MMP_RS06725 0.71 -0.7 1,292,357 + MMP_RS06725 0.80 -2.6 1,292,390 - MMP_RS06725 0.82 -1.3 1,292,793 + MMP_RS06730 0.17 -3.1 1,293,000 + MMP_RS06730 0.44 -4.2 1,293,067 - MMP_RS06730 0.53 -2.5 1,293,118 + MMP_RS06730 0.59 +0.3 1,293,368 - -3.2 1,293,399 - -2.1 1,293,438 - -1.0 1,293,492 + -0.8 1,293,496 - +0.3 1,293,525 + +0.1 1,293,525 + +0.9 1,293,553 - +0.8 1,293,553 - +1.3 1,293,554 + -0.1 1,293,633 - MMP_RS06735 0.13 +0.3 1,293,856 + MMP_RS06735 0.32 -0.3 1,294,295 - MMP_RS06735 0.70 +1.6 1,294,300 + MMP_RS06735 0.71 -0.2 1,294,308 - MMP_RS06735 0.72 -0.1
Or see this region's nucleotide sequence