Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03210 and MMP_RS03215 are separated by 52 nucleotides MMP_RS03215 and MMP_RS03220 are separated by 1 nucleotides MMP_RS03220 and MMP_RS08955 are separated by 8 nucleotides MMP_RS08955 and MMP_RS03225 are separated by 80 nucleotides
MMP_RS03210: MMP_RS03210 - tRNA guanosine(15) transglycosylase TgtA, at 604,972 to 606,921
_RS03210
MMP_RS03215: MMP_RS03215 - MBL fold metallo-hydrolase, at 606,974 to 607,516
_RS03215
MMP_RS03220: MMP_RS03220 - RNA 2' 3'-cyclic phosphodiesterase, at 607,518 to 608,042
_RS03220
MMP_RS08955: MMP_RS08955 - hypothetical protein, at 608,051 to 608,197
_RS08955
MMP_RS03225: MMP_RS03225 - acetolactate decarboxylase, at 608,278 to 609,078
_RS03225
Position (kb) 607
608
609 Strain fitness (log2 ratio) -1
0
1 at 606.665 kb on - strand, within MMP_RS03210 at 606.698 kb on - strand, within MMP_RS03210 at 606.848 kb on - strand at 606.851 kb on - strand at 606.851 kb on - strand at 606.934 kb on + strand at 606.944 kb on - strand at 606.990 kb on + strand at 607.054 kb on - strand, within MMP_RS03215 at 607.055 kb on + strand, within MMP_RS03215 at 607.059 kb on + strand, within MMP_RS03215 at 607.170 kb on + strand, within MMP_RS03215 at 607.172 kb on + strand, within MMP_RS03215 at 607.172 kb on + strand, within MMP_RS03215 at 607.174 kb on - strand, within MMP_RS03215 at 607.175 kb on + strand, within MMP_RS03215 at 607.177 kb on + strand, within MMP_RS03215 at 607.177 kb on + strand, within MMP_RS03215 at 607.177 kb on + strand, within MMP_RS03215 at 607.177 kb on + strand, within MMP_RS03215 at 607.241 kb on + strand, within MMP_RS03215 at 607.330 kb on + strand, within MMP_RS03215 at 607.343 kb on - strand, within MMP_RS03215 at 607.405 kb on - strand, within MMP_RS03215 at 607.414 kb on + strand, within MMP_RS03215 at 607.414 kb on + strand, within MMP_RS03215 at 607.453 kb on + strand, within MMP_RS03215 at 607.474 kb on + strand at 607.474 kb on + strand at 607.520 kb on + strand at 607.559 kb on - strand at 607.560 kb on + strand at 607.560 kb on + strand at 607.569 kb on + strand at 607.927 kb on + strand, within MMP_RS03220 at 607.927 kb on + strand, within MMP_RS03220 at 608.230 kb on - strand at 608.245 kb on + strand at 608.262 kb on + strand at 608.331 kb on - strand at 608.462 kb on - strand, within MMP_RS03225 at 608.483 kb on - strand, within MMP_RS03225 at 608.536 kb on - strand, within MMP_RS03225 at 608.536 kb on - strand, within MMP_RS03225 at 608.808 kb on + strand, within MMP_RS03225
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 606,665 - MMP_RS03210 0.87 -0.3 606,698 - MMP_RS03210 0.89 -0.0 606,848 - -0.3 606,851 - +0.4 606,851 - +0.6 606,934 + +0.7 606,944 - +0.4 606,990 + +0.7 607,054 - MMP_RS03215 0.15 +0.8 607,055 + MMP_RS03215 0.15 +0.9 607,059 + MMP_RS03215 0.16 +0.2 607,170 + MMP_RS03215 0.36 +0.4 607,172 + MMP_RS03215 0.36 -0.9 607,172 + MMP_RS03215 0.36 +0.7 607,174 - MMP_RS03215 0.37 +1.3 607,175 + MMP_RS03215 0.37 +0.7 607,177 + MMP_RS03215 0.37 +0.8 607,177 + MMP_RS03215 0.37 +1.2 607,177 + MMP_RS03215 0.37 +0.2 607,177 + MMP_RS03215 0.37 +0.7 607,241 + MMP_RS03215 0.49 +0.2 607,330 + MMP_RS03215 0.66 +1.1 607,343 - MMP_RS03215 0.68 +1.7 607,405 - MMP_RS03215 0.79 +0.6 607,414 + MMP_RS03215 0.81 +1.0 607,414 + MMP_RS03215 0.81 +1.2 607,453 + MMP_RS03215 0.88 +0.8 607,474 + +0.1 607,474 + +0.8 607,520 + +0.5 607,559 - +0.7 607,560 + +0.9 607,560 + -0.4 607,569 + +0.6 607,927 + MMP_RS03220 0.78 +0.3 607,927 + MMP_RS03220 0.78 +0.5 608,230 - -0.0 608,245 + +0.4 608,262 + +0.7 608,331 - +0.3 608,462 - MMP_RS03225 0.23 +0.5 608,483 - MMP_RS03225 0.26 -0.2 608,536 - MMP_RS03225 0.32 -0.4 608,536 - MMP_RS03225 0.32 -0.8 608,808 + MMP_RS03225 0.66 +0.2
Or see this region's nucleotide sequence