Experiment: H2 McCas with 10 mM polysulfide
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01215 and MMP_RS01220 are separated by 78 nucleotides MMP_RS01220 and MMP_RS01225 are separated by 9 nucleotides MMP_RS01225 and MMP_RS01230 are separated by 9 nucleotides
MMP_RS01215: MMP_RS01215 - sodium/proline symporter PutP, at 229,031 to 230,509
_RS01215
MMP_RS01220: MMP_RS01220 - hypothetical protein, at 230,588 to 230,827
_RS01220
MMP_RS01225: MMP_RS01225 - DUF5379 domain-containing protein, at 230,837 to 231,115
_RS01225
MMP_RS01230: MMP_RS01230 - glutamate-1-semialdehyde 2 1-aminomutase, at 231,125 to 232,408
_RS01230
Position (kb) 230
231
232 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 229.965 kb on - strand, within MMP_RS01215 at 229.966 kb on + strand, within MMP_RS01215 at 230.437 kb on + strand at 230.544 kb on + strand at 230.544 kb on + strand at 230.544 kb on + strand at 230.547 kb on - strand at 230.548 kb on + strand at 230.557 kb on + strand at 230.557 kb on + strand at 230.588 kb on + strand at 230.637 kb on - strand, within MMP_RS01220 at 230.687 kb on + strand, within MMP_RS01220 at 230.705 kb on + strand, within MMP_RS01220 at 230.720 kb on - strand, within MMP_RS01220 at 230.724 kb on - strand, within MMP_RS01220 at 230.724 kb on - strand, within MMP_RS01220 at 230.743 kb on + strand, within MMP_RS01220 at 230.755 kb on - strand, within MMP_RS01220 at 230.756 kb on + strand, within MMP_RS01220 at 230.756 kb on + strand, within MMP_RS01220 at 230.756 kb on + strand, within MMP_RS01220 at 230.759 kb on - strand, within MMP_RS01220 at 230.760 kb on + strand, within MMP_RS01220 at 230.762 kb on + strand, within MMP_RS01220 at 230.762 kb on + strand, within MMP_RS01220 at 230.792 kb on + strand, within MMP_RS01220 at 230.844 kb on - strand at 230.844 kb on - strand at 230.906 kb on - strand, within MMP_RS01225 at 230.906 kb on - strand, within MMP_RS01225 at 230.912 kb on - strand, within MMP_RS01225 at 230.912 kb on - strand, within MMP_RS01225 at 230.912 kb on - strand, within MMP_RS01225 at 230.955 kb on - strand, within MMP_RS01225 at 230.974 kb on + strand, within MMP_RS01225 at 230.977 kb on - strand, within MMP_RS01225 at 230.977 kb on - strand, within MMP_RS01225 at 231.095 kb on - strand at 231.162 kb on - strand at 231.357 kb on - strand at 231.734 kb on + strand at 232.022 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM polysulfide remove 229,965 - MMP_RS01215 0.63 +0.5 229,966 + MMP_RS01215 0.63 +0.5 230,437 + +0.6 230,544 + -0.3 230,544 + -0.1 230,544 + +0.4 230,547 - -0.4 230,548 + -0.0 230,557 + -0.5 230,557 + +0.0 230,588 + -0.8 230,637 - MMP_RS01220 0.20 -0.9 230,687 + MMP_RS01220 0.41 -1.6 230,705 + MMP_RS01220 0.49 -2.1 230,720 - MMP_RS01220 0.55 -1.0 230,724 - MMP_RS01220 0.57 -1.3 230,724 - MMP_RS01220 0.57 -3.1 230,743 + MMP_RS01220 0.65 -4.3 230,755 - MMP_RS01220 0.70 -2.6 230,756 + MMP_RS01220 0.70 -1.8 230,756 + MMP_RS01220 0.70 -2.6 230,756 + MMP_RS01220 0.70 -2.6 230,759 - MMP_RS01220 0.71 -3.0 230,760 + MMP_RS01220 0.72 -1.7 230,762 + MMP_RS01220 0.72 -2.4 230,762 + MMP_RS01220 0.72 -0.6 230,792 + MMP_RS01220 0.85 -2.0 230,844 - -3.6 230,844 - -1.7 230,906 - MMP_RS01225 0.25 -2.6 230,906 - MMP_RS01225 0.25 -2.0 230,912 - MMP_RS01225 0.27 -3.1 230,912 - MMP_RS01225 0.27 -2.8 230,912 - MMP_RS01225 0.27 -5.0 230,955 - MMP_RS01225 0.42 -2.5 230,974 + MMP_RS01225 0.49 -4.3 230,977 - MMP_RS01225 0.50 -3.0 230,977 - MMP_RS01225 0.50 -2.9 231,095 - -2.8 231,162 - -3.0 231,357 - -2.8 231,734 + -1.9 232,022 + -3.1
Or see this region's nucleotide sequence