Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4141 and BT4142 are separated by 819 nucleotides BT4142 and BT4143 overlap by 4 nucleotides BT4143 and BT4144 are separated by 138 nucleotides
BT4141: BT4141 - hypothetical protein (NCBI ptt file), at 5,436,642 to 5,436,974
BT4141
BT4142: BT4142 - conserved hypothetical protein (NCBI ptt file), at 5,437,794 to 5,438,921
BT4142
BT4143: BT4143 - putative iron-sulfur cluster binding protein (NCBI ptt file), at 5,438,918 to 5,439,598
BT4143
BT4144: BT4144 - transposase (NCBI ptt file), at 5,439,737 to 5,440,846
BT4144
Position (kb) 5437
5438
5439 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5436.801 kb on - strand, within BT4141 at 5436.845 kb on - strand, within BT4141 at 5436.855 kb on + strand, within BT4141 at 5436.856 kb on - strand, within BT4141 at 5436.876 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.904 kb on - strand, within BT4141 at 5436.955 kb on + strand at 5436.955 kb on + strand at 5437.077 kb on - strand at 5437.143 kb on + strand at 5437.183 kb on + strand at 5437.203 kb on - strand at 5437.249 kb on + strand at 5437.252 kb on + strand at 5437.269 kb on - strand at 5437.269 kb on - strand at 5437.282 kb on - strand at 5437.296 kb on + strand at 5437.546 kb on - strand at 5437.546 kb on - strand at 5437.546 kb on - strand at 5437.546 kb on - strand at 5437.549 kb on + strand at 5437.549 kb on + strand at 5437.550 kb on - strand at 5437.591 kb on + strand at 5437.696 kb on + strand at 5437.697 kb on - strand at 5437.697 kb on - strand at 5437.706 kb on - strand at 5437.706 kb on - strand at 5437.759 kb on + strand at 5437.760 kb on - strand at 5437.766 kb on + strand at 5437.819 kb on - strand at 5437.888 kb on - strand at 5437.923 kb on + strand, within BT4142 at 5437.950 kb on + strand, within BT4142 at 5437.980 kb on + strand, within BT4142 at 5437.997 kb on - strand, within BT4142 at 5438.028 kb on - strand, within BT4142 at 5438.092 kb on - strand, within BT4142 at 5438.188 kb on - strand, within BT4142 at 5438.238 kb on + strand, within BT4142 at 5438.281 kb on + strand, within BT4142 at 5438.294 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.340 kb on - strand, within BT4142 at 5438.379 kb on - strand, within BT4142 at 5438.451 kb on - strand, within BT4142 at 5438.482 kb on - strand, within BT4142 at 5438.482 kb on - strand, within BT4142 at 5438.546 kb on - strand, within BT4142 at 5438.562 kb on + strand, within BT4142 at 5438.586 kb on - strand, within BT4142 at 5438.586 kb on - strand, within BT4142 at 5438.589 kb on + strand, within BT4142 at 5438.594 kb on - strand, within BT4142 at 5438.610 kb on + strand, within BT4142 at 5438.611 kb on - strand, within BT4142 at 5438.611 kb on - strand, within BT4142 at 5438.646 kb on - strand, within BT4142 at 5438.656 kb on + strand, within BT4142 at 5438.656 kb on + strand, within BT4142 at 5438.657 kb on - strand, within BT4142 at 5438.663 kb on - strand, within BT4142 at 5438.700 kb on + strand, within BT4142 at 5438.700 kb on + strand, within BT4142 at 5438.721 kb on + strand, within BT4142 at 5438.722 kb on - strand, within BT4142 at 5438.726 kb on + strand, within BT4142 at 5438.728 kb on + strand, within BT4142 at 5438.728 kb on + strand, within BT4142 at 5438.746 kb on + strand, within BT4142 at 5438.747 kb on - strand, within BT4142 at 5438.748 kb on + strand, within BT4142 at 5438.749 kb on - strand, within BT4142 at 5438.749 kb on - strand, within BT4142 at 5438.762 kb on + strand, within BT4142 at 5438.767 kb on + strand, within BT4142 at 5438.769 kb on + strand, within BT4142 at 5438.777 kb on + strand, within BT4142 at 5438.782 kb on - strand, within BT4142 at 5438.841 kb on + strand at 5438.849 kb on + strand at 5438.849 kb on + strand at 5438.917 kb on + strand at 5438.923 kb on + strand at 5438.974 kb on + strand at 5438.976 kb on + strand at 5438.980 kb on + strand at 5438.981 kb on - strand at 5438.998 kb on + strand, within BT4143 at 5439.007 kb on + strand, within BT4143 at 5439.025 kb on + strand, within BT4143 at 5439.026 kb on - strand, within BT4143 at 5439.028 kb on - strand, within BT4143 at 5439.030 kb on + strand, within BT4143 at 5439.031 kb on - strand, within BT4143 at 5439.089 kb on - strand, within BT4143 at 5439.110 kb on + strand, within BT4143 at 5439.114 kb on + strand, within BT4143 at 5439.128 kb on - strand, within BT4143 at 5439.141 kb on + strand, within BT4143 at 5439.142 kb on - strand, within BT4143 at 5439.153 kb on + strand, within BT4143 at 5439.181 kb on + strand, within BT4143 at 5439.181 kb on + strand, within BT4143 at 5439.181 kb on + strand, within BT4143 at 5439.181 kb on + strand, within BT4143 at 5439.280 kb on + strand, within BT4143 at 5439.389 kb on + strand, within BT4143 at 5439.422 kb on - strand, within BT4143 at 5439.425 kb on - strand, within BT4143 at 5439.439 kb on - strand, within BT4143 at 5439.477 kb on + strand, within BT4143 at 5439.497 kb on - strand, within BT4143 at 5439.507 kb on + strand, within BT4143 at 5439.507 kb on + strand, within BT4143 at 5439.507 kb on + strand, within BT4143 at 5439.508 kb on - strand, within BT4143 at 5439.508 kb on - strand, within BT4143 at 5439.508 kb on - strand, within BT4143 at 5439.508 kb on - strand, within BT4143 at 5439.509 kb on + strand, within BT4143 at 5439.510 kb on - strand, within BT4143 at 5439.520 kb on + strand, within BT4143 at 5439.563 kb on + strand at 5439.563 kb on + strand at 5439.563 kb on + strand at 5439.564 kb on - strand at 5439.589 kb on - strand at 5439.596 kb on + strand at 5439.606 kb on - strand at 5439.636 kb on - strand at 5439.643 kb on - strand at 5439.650 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 5,436,801 - BT4141 0.48 +0.4 5,436,845 - BT4141 0.61 +0.3 5,436,855 + BT4141 0.64 -0.0 5,436,856 - BT4141 0.64 -1.6 5,436,876 - BT4141 0.70 -1.0 5,436,902 - BT4141 0.78 -3.0 5,436,902 - BT4141 0.78 -0.7 5,436,902 - BT4141 0.78 -2.7 5,436,902 - BT4141 0.78 -0.0 5,436,904 - BT4141 0.79 -1.0 5,436,955 + -1.8 5,436,955 + -1.0 5,437,077 - +0.4 5,437,143 + +2.3 5,437,183 + -3.3 5,437,203 - +1.0 5,437,249 + -0.4 5,437,252 + +0.5 5,437,269 - -1.5 5,437,269 - -0.2 5,437,282 - -0.4 5,437,296 + -2.0 5,437,546 - -2.4 5,437,546 - +0.8 5,437,546 - -0.0 5,437,546 - +0.6 5,437,549 + -2.0 5,437,549 + -0.5 5,437,550 - -0.1 5,437,591 + -2.4 5,437,696 + -1.0 5,437,697 - -0.4 5,437,697 - -1.0 5,437,706 - +0.1 5,437,706 - -3.4 5,437,759 + -2.0 5,437,760 - -0.0 5,437,766 + +0.7 5,437,819 - +0.1 5,437,888 - -3.2 5,437,923 + BT4142 0.11 -2.0 5,437,950 + BT4142 0.14 +0.2 5,437,980 + BT4142 0.16 -0.4 5,437,997 - BT4142 0.18 -0.4 5,438,028 - BT4142 0.21 -2.2 5,438,092 - BT4142 0.26 -0.0 5,438,188 - BT4142 0.35 -1.6 5,438,238 + BT4142 0.39 -1.6 5,438,281 + BT4142 0.43 -1.6 5,438,294 - BT4142 0.44 +0.8 5,438,340 - BT4142 0.48 +0.2 5,438,340 - BT4142 0.48 -0.0 5,438,340 - BT4142 0.48 -1.0 5,438,340 - BT4142 0.48 +0.4 5,438,340 - BT4142 0.48 +0.4 5,438,379 - BT4142 0.52 -0.0 5,438,451 - BT4142 0.58 -2.4 5,438,482 - BT4142 0.61 -3.4 5,438,482 - BT4142 0.61 +1.0 5,438,546 - BT4142 0.67 -0.7 5,438,562 + BT4142 0.68 -0.0 5,438,586 - BT4142 0.70 +1.4 5,438,586 - BT4142 0.70 -0.2 5,438,589 + BT4142 0.70 -1.6 5,438,594 - BT4142 0.71 -2.4 5,438,610 + BT4142 0.72 -2.0 5,438,611 - BT4142 0.72 -1.3 5,438,611 - BT4142 0.72 -0.6 5,438,646 - BT4142 0.76 -2.0 5,438,656 + BT4142 0.76 -1.6 5,438,656 + BT4142 0.76 -1.5 5,438,657 - BT4142 0.77 -3.4 5,438,663 - BT4142 0.77 -3.2 5,438,700 + BT4142 0.80 +0.2 5,438,700 + BT4142 0.80 -0.0 5,438,721 + BT4142 0.82 +0.4 5,438,722 - BT4142 0.82 +1.0 5,438,726 + BT4142 0.83 +0.4 5,438,728 + BT4142 0.83 -1.0 5,438,728 + BT4142 0.83 -1.6 5,438,746 + BT4142 0.84 -0.7 5,438,747 - BT4142 0.84 -1.4 5,438,748 + BT4142 0.85 -1.6 5,438,749 - BT4142 0.85 -0.2 5,438,749 - BT4142 0.85 -1.0 5,438,762 + BT4142 0.86 -0.0 5,438,767 + BT4142 0.86 +0.7 5,438,769 + BT4142 0.86 +2.8 5,438,777 + BT4142 0.87 -1.0 5,438,782 - BT4142 0.88 -1.3 5,438,841 + +0.2 5,438,849 + +1.3 5,438,849 + -1.2 5,438,917 + -0.9 5,438,923 + -0.1 5,438,974 + -0.1 5,438,976 + -0.7 5,438,980 + -0.6 5,438,981 - -3.5 5,438,998 + BT4143 0.12 -1.1 5,439,007 + BT4143 0.13 +0.4 5,439,025 + BT4143 0.16 -2.1 5,439,026 - BT4143 0.16 -1.6 5,439,028 - BT4143 0.16 +0.4 5,439,030 + BT4143 0.16 -2.1 5,439,031 - BT4143 0.17 -1.1 5,439,089 - BT4143 0.25 -0.7 5,439,110 + BT4143 0.28 -3.5 5,439,114 + BT4143 0.29 -1.6 5,439,128 - BT4143 0.31 +0.9 5,439,141 + BT4143 0.33 +1.6 5,439,142 - BT4143 0.33 -0.1 5,439,153 + BT4143 0.35 -1.2 5,439,181 + BT4143 0.39 -0.1 5,439,181 + BT4143 0.39 -1.1 5,439,181 + BT4143 0.39 +1.7 5,439,181 + BT4143 0.39 -2.4 5,439,280 + BT4143 0.53 -0.5 5,439,389 + BT4143 0.69 -2.1 5,439,422 - BT4143 0.74 -0.1 5,439,425 - BT4143 0.74 -0.1 5,439,439 - BT4143 0.77 -0.3 5,439,477 + BT4143 0.82 +0.5 5,439,497 - BT4143 0.85 -2.9 5,439,507 + BT4143 0.86 -2.1 5,439,507 + BT4143 0.86 -1.6 5,439,507 + BT4143 0.86 -1.1 5,439,508 - BT4143 0.87 -0.7 5,439,508 - BT4143 0.87 -0.1 5,439,508 - BT4143 0.87 -0.8 5,439,508 - BT4143 0.87 -0.6 5,439,509 + BT4143 0.87 +0.0 5,439,510 - BT4143 0.87 -0.9 5,439,520 + BT4143 0.88 -1.9 5,439,563 + +0.3 5,439,563 + -0.6 5,439,563 + -1.6 5,439,564 - +0.7 5,439,589 - -2.6 5,439,596 + -2.9 5,439,606 - -1.6 5,439,636 - -0.6 5,439,643 - +0.9 5,439,650 - -2.4
Or see this region's nucleotide sequence