Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4074 and BT4075 are separated by 765 nucleotides BT4075 and BT4076 are separated by 70 nucleotides
BT4074: BT4074 - beta-mannosidase precursor (NCBI ptt file), at 5,309,556 to 5,312,639
BT4074
BT4075: BT4075 - conserved hypothetical protein (NCBI ptt file), at 5,313,405 to 5,314,808
BT4075
BT4076: BT4076 - alpha-rhamnosidase (NCBI ptt file), at 5,314,879 to 5,317,557
BT4076
Position (kb)
5313
5314
5315 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5312.466 kb on - strand at 5312.511 kb on + strand at 5312.512 kb on - strand at 5312.515 kb on + strand at 5312.516 kb on - strand at 5312.543 kb on + strand at 5312.604 kb on + strand at 5312.610 kb on - strand at 5312.633 kb on + strand at 5312.635 kb on + strand at 5312.641 kb on - strand at 5312.642 kb on + strand at 5312.642 kb on + strand at 5312.643 kb on - strand at 5312.654 kb on + strand at 5312.729 kb on + strand at 5312.730 kb on - strand at 5312.781 kb on + strand at 5312.898 kb on - strand at 5312.898 kb on - strand at 5312.920 kb on - strand at 5312.920 kb on - strand at 5312.951 kb on + strand at 5312.962 kb on - strand at 5312.974 kb on + strand at 5312.999 kb on + strand at 5313.021 kb on - strand at 5313.035 kb on - strand at 5313.038 kb on + strand at 5313.045 kb on - strand at 5313.086 kb on + strand at 5313.086 kb on + strand at 5313.101 kb on - strand at 5313.132 kb on - strand at 5313.132 kb on - strand at 5313.152 kb on + strand at 5313.161 kb on + strand at 5313.187 kb on - strand at 5313.443 kb on - strand at 5313.448 kb on + strand at 5313.465 kb on - strand at 5313.474 kb on + strand at 5313.474 kb on + strand at 5313.536 kb on - strand at 5313.578 kb on + strand, within BT4075 at 5313.610 kb on + strand, within BT4075 at 5313.693 kb on + strand, within BT4075 at 5313.735 kb on - strand, within BT4075 at 5313.755 kb on + strand, within BT4075 at 5313.834 kb on - strand, within BT4075 at 5313.834 kb on - strand, within BT4075 at 5313.853 kb on + strand, within BT4075 at 5313.868 kb on - strand, within BT4075 at 5313.868 kb on - strand, within BT4075 at 5313.879 kb on - strand, within BT4075 at 5313.921 kb on - strand, within BT4075 at 5313.921 kb on - strand, within BT4075 at 5313.926 kb on - strand, within BT4075 at 5313.932 kb on - strand, within BT4075 at 5314.050 kb on - strand, within BT4075 at 5314.055 kb on - strand, within BT4075 at 5314.058 kb on + strand, within BT4075 at 5314.185 kb on - strand, within BT4075 at 5314.229 kb on - strand, within BT4075 at 5314.261 kb on - strand, within BT4075 at 5314.320 kb on - strand, within BT4075 at 5314.337 kb on + strand, within BT4075 at 5314.405 kb on + strand, within BT4075 at 5314.406 kb on - strand, within BT4075 at 5314.406 kb on - strand, within BT4075 at 5314.622 kb on + strand, within BT4075 at 5314.642 kb on - strand, within BT4075 at 5314.684 kb on - strand at 5314.724 kb on + strand at 5314.725 kb on - strand at 5314.767 kb on - strand at 5314.810 kb on + strand at 5314.852 kb on - strand at 5314.908 kb on - strand at 5314.914 kb on - strand at 5314.914 kb on - strand at 5314.914 kb on - strand at 5314.947 kb on - strand at 5314.947 kb on - strand at 5315.112 kb on + strand at 5315.223 kb on - strand, within BT4076 at 5315.334 kb on + strand, within BT4076 at 5315.352 kb on + strand, within BT4076 at 5315.423 kb on - strand, within BT4076 at 5315.441 kb on - strand, within BT4076 at 5315.451 kb on - strand, within BT4076 at 5315.451 kb on - strand, within BT4076 at 5315.452 kb on + strand, within BT4076 at 5315.452 kb on + strand, within BT4076 at 5315.487 kb on + strand, within BT4076 at 5315.512 kb on - strand, within BT4076 at 5315.534 kb on - strand, within BT4076 at 5315.534 kb on - strand, within BT4076 at 5315.540 kb on + strand, within BT4076 at 5315.541 kb on - strand, within BT4076 at 5315.541 kb on - strand, within BT4076 at 5315.546 kb on - strand, within BT4076 at 5315.553 kb on + strand, within BT4076 at 5315.659 kb on - strand, within BT4076 at 5315.685 kb on - strand, within BT4076 at 5315.742 kb on + strand, within BT4076 at 5315.789 kb on + strand, within BT4076
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 5,312,466 - -3.5 5,312,511 + -1.0 5,312,512 - +0.2 5,312,515 + -1.6 5,312,516 - -1.6 5,312,543 + -1.6 5,312,604 + -0.7 5,312,610 - -0.7 5,312,633 + -0.8 5,312,635 + -2.4 5,312,641 - -1.0 5,312,642 + -0.0 5,312,642 + -0.0 5,312,643 - -0.0 5,312,654 + -1.6 5,312,729 + -0.0 5,312,730 - -0.0 5,312,781 + -0.0 5,312,898 - -3.9 5,312,898 - -3.2 5,312,920 - -0.4 5,312,920 - -1.0 5,312,951 + -0.9 5,312,962 - +0.4 5,312,974 + -2.4 5,312,999 + -2.5 5,313,021 - -1.4 5,313,035 - -0.4 5,313,038 + -3.4 5,313,045 - -0.0 5,313,086 + -0.0 5,313,086 + -2.0 5,313,101 - -1.6 5,313,132 - -1.0 5,313,132 - -1.6 5,313,152 + -0.0 5,313,161 + -2.6 5,313,187 - -1.2 5,313,443 - -1.3 5,313,448 + -0.0 5,313,465 - -1.6 5,313,474 + -3.4 5,313,474 + -1.2 5,313,536 - -2.6 5,313,578 + BT4075 0.12 -1.6 5,313,610 + BT4075 0.15 -2.9 5,313,693 + BT4075 0.21 -3.0 5,313,735 - BT4075 0.24 -0.0 5,313,755 + BT4075 0.25 -3.2 5,313,834 - BT4075 0.31 -0.7 5,313,834 - BT4075 0.31 -2.4 5,313,853 + BT4075 0.32 -1.6 5,313,868 - BT4075 0.33 -1.8 5,313,868 - BT4075 0.33 -2.5 5,313,879 - BT4075 0.34 -1.0 5,313,921 - BT4075 0.37 -2.0 5,313,921 - BT4075 0.37 +0.2 5,313,926 - BT4075 0.37 +1.1 5,313,932 - BT4075 0.38 -0.0 5,314,050 - BT4075 0.46 -3.0 5,314,055 - BT4075 0.46 -2.6 5,314,058 + BT4075 0.47 -1.6 5,314,185 - BT4075 0.56 -1.0 5,314,229 - BT4075 0.59 -0.6 5,314,261 - BT4075 0.61 -3.2 5,314,320 - BT4075 0.65 -1.1 5,314,337 + BT4075 0.66 -3.0 5,314,405 + BT4075 0.71 -2.3 5,314,406 - BT4075 0.71 -2.4 5,314,406 - BT4075 0.71 -2.2 5,314,622 + BT4075 0.87 -0.0 5,314,642 - BT4075 0.88 -0.0 5,314,684 - -0.1 5,314,724 + -0.0 5,314,725 - -1.4 5,314,767 - -2.4 5,314,810 + -1.5 5,314,852 - +1.5 5,314,908 - -1.6 5,314,914 - -0.2 5,314,914 - +0.4 5,314,914 - -1.0 5,314,947 - -2.4 5,314,947 - -4.1 5,315,112 + -1.0 5,315,223 - BT4076 0.13 -0.5 5,315,334 + BT4076 0.17 -2.8 5,315,352 + BT4076 0.18 -4.0 5,315,423 - BT4076 0.20 -2.6 5,315,441 - BT4076 0.21 +0.5 5,315,451 - BT4076 0.21 +0.7 5,315,451 - BT4076 0.21 -1.0 5,315,452 + BT4076 0.21 +1.0 5,315,452 + BT4076 0.21 -3.0 5,315,487 + BT4076 0.23 +0.5 5,315,512 - BT4076 0.24 -3.9 5,315,534 - BT4076 0.24 -2.6 5,315,534 - BT4076 0.24 +0.7 5,315,540 + BT4076 0.25 -0.0 5,315,541 - BT4076 0.25 -0.2 5,315,541 - BT4076 0.25 -2.0 5,315,546 - BT4076 0.25 -1.6 5,315,553 + BT4076 0.25 -0.0 5,315,659 - BT4076 0.29 -1.0 5,315,685 - BT4076 0.30 -2.0 5,315,742 + BT4076 0.32 +1.0 5,315,789 + BT4076 0.34 -2.4
Or see this region's nucleotide sequence