Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4022 and BT4023 are separated by 150 nucleotides BT4023 and BT4024 overlap by 4 nucleotides BT4024 and BT4025 are separated by 21 nucleotides BT4025 and BT4026 overlap by 4 nucleotides
BT4022: BT4022 - integrase (NCBI ptt file), at 5,238,754 to 5,239,983
BT4022
BT4023: BT4023 - transposase (NCBI ptt file), at 5,240,134 to 5,240,490
BT4023
BT4024: BT4024 - hypothetical protein (NCBI ptt file), at 5,240,487 to 5,240,747
BT4024
BT4025: BT4025 - hypothetical protein (NCBI ptt file), at 5,240,769 to 5,241,065
BT4025
BT4026: BT4026 - conserved hypothetical protein (NCBI ptt file), at 5,241,062 to 5,242,060
BT4026
Position (kb) 5240
5241 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5239.159 kb on - strand, within BT4022 at 5239.162 kb on + strand, within BT4022 at 5239.163 kb on - strand, within BT4022 at 5239.163 kb on - strand, within BT4022 at 5239.196 kb on + strand, within BT4022 at 5239.211 kb on + strand, within BT4022 at 5239.243 kb on + strand, within BT4022 at 5239.344 kb on + strand, within BT4022 at 5239.373 kb on + strand, within BT4022 at 5239.386 kb on - strand, within BT4022 at 5239.386 kb on - strand, within BT4022 at 5239.386 kb on - strand, within BT4022 at 5239.529 kb on + strand, within BT4022 at 5239.529 kb on + strand, within BT4022 at 5239.568 kb on + strand, within BT4022 at 5239.569 kb on - strand at 5239.569 kb on - strand, within BT4022 at 5239.705 kb on - strand, within BT4022 at 5239.961 kb on - strand at 5240.051 kb on - strand at 5240.194 kb on - strand, within BT4023 at 5240.202 kb on - strand, within BT4023 at 5240.223 kb on - strand, within BT4023 at 5240.236 kb on - strand, within BT4023 at 5240.266 kb on - strand, within BT4023 at 5240.266 kb on - strand, within BT4023 at 5240.268 kb on - strand, within BT4023 at 5240.356 kb on - strand, within BT4023 at 5240.554 kb on - strand, within BT4024 at 5240.619 kb on - strand, within BT4024 at 5240.666 kb on - strand, within BT4024 at 5240.666 kb on - strand, within BT4024 at 5240.717 kb on - strand, within BT4024 at 5240.728 kb on - strand at 5240.743 kb on - strand at 5240.753 kb on - strand at 5240.763 kb on - strand at 5240.922 kb on - strand, within BT4025 at 5240.986 kb on - strand, within BT4025 at 5241.034 kb on - strand, within BT4025 at 5241.067 kb on + strand at 5241.068 kb on - strand at 5241.086 kb on + strand at 5241.123 kb on + strand at 5241.124 kb on - strand at 5241.158 kb on + strand at 5241.167 kb on + strand, within BT4026 at 5241.209 kb on + strand, within BT4026 at 5241.210 kb on - strand, within BT4026 at 5241.345 kb on + strand, within BT4026 at 5241.345 kb on + strand, within BT4026 at 5241.415 kb on - strand, within BT4026 at 5241.436 kb on - strand, within BT4026
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 5,239,159 - BT4022 0.33 +1.0 5,239,162 + BT4022 0.33 -3.2 5,239,163 - BT4022 0.33 -1.0 5,239,163 - BT4022 0.33 -0.0 5,239,196 + BT4022 0.36 -0.0 5,239,211 + BT4022 0.37 -2.0 5,239,243 + BT4022 0.40 +0.3 5,239,344 + BT4022 0.48 -1.0 5,239,373 + BT4022 0.50 -0.0 5,239,386 - BT4022 0.51 -2.8 5,239,386 - BT4022 0.51 -2.3 5,239,386 - BT4022 0.51 -3.4 5,239,529 + BT4022 0.63 -1.0 5,239,529 + BT4022 0.63 -0.0 5,239,568 + BT4022 0.66 -4.1 5,239,569 - +1.6 5,239,569 - BT4022 0.66 -0.7 5,239,705 - BT4022 0.77 -4.3 5,239,961 - -0.9 5,240,051 - -1.3 5,240,194 - BT4023 0.17 -1.6 5,240,202 - BT4023 0.19 -1.2 5,240,223 - BT4023 0.25 +0.7 5,240,236 - BT4023 0.29 -0.0 5,240,266 - BT4023 0.37 -0.0 5,240,266 - BT4023 0.37 -3.9 5,240,268 - BT4023 0.38 +0.6 5,240,356 - BT4023 0.62 +1.6 5,240,554 - BT4024 0.26 -1.6 5,240,619 - BT4024 0.51 +1.5 5,240,666 - BT4024 0.69 -3.6 5,240,666 - BT4024 0.69 -2.3 5,240,717 - BT4024 0.88 -0.2 5,240,728 - -2.6 5,240,743 - -1.1 5,240,753 - -1.0 5,240,763 - -1.1 5,240,922 - BT4025 0.52 -0.0 5,240,986 - BT4025 0.73 -1.6 5,241,034 - BT4025 0.89 -2.1 5,241,067 + -0.0 5,241,068 - -0.4 5,241,086 + -0.7 5,241,123 + -4.3 5,241,124 - +0.1 5,241,158 + +0.3 5,241,167 + BT4026 0.11 -1.2 5,241,209 + BT4026 0.15 -1.0 5,241,210 - BT4026 0.15 +0.8 5,241,345 + BT4026 0.28 -1.0 5,241,345 + BT4026 0.28 -1.0 5,241,415 - BT4026 0.35 -0.1 5,241,436 - BT4026 0.37 -1.0
Or see this region's nucleotide sequence