Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3401 and BT3402 are separated by 154 nucleotides BT3402 and BT3403 are separated by 2 nucleotides BT3403 and BT3404 are separated by 52 nucleotides
BT3401: BT3401 - conserved hypothetical protein (NCBI ptt file), at 4,380,147 to 4,381,292
BT3401
BT3402: BT3402 - conserved hypothetical protein (NCBI ptt file), at 4,381,447 to 4,381,914
BT3402
BT3403: BT3403 - putative nitrogen utilization substance protein (NCBI ptt file), at 4,381,917 to 4,383,236
BT3403
BT3404: BT3404 - translation initiation factor IF-2 (NCBI ptt file), at 4,383,289 to 4,386,411
BT3404
Position (kb)
4381
4382
4383
4384 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4380.917 kb on + strand, within BT3401 at 4380.940 kb on - strand, within BT3401 at 4380.958 kb on - strand, within BT3401 at 4380.987 kb on + strand, within BT3401 at 4381.009 kb on - strand, within BT3401 at 4381.066 kb on - strand, within BT3401 at 4381.167 kb on - strand, within BT3401 at 4381.291 kb on - strand at 4381.291 kb on - strand at 4381.294 kb on + strand at 4381.294 kb on + strand at 4381.295 kb on - strand at 4381.313 kb on + strand at 4381.314 kb on - strand at 4381.315 kb on + strand at 4381.316 kb on - strand at 4381.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 4,380,917 + BT3401 0.67 -1.0 4,380,940 - BT3401 0.69 -3.3 4,380,958 - BT3401 0.71 -1.6 4,380,987 + BT3401 0.73 -1.4 4,381,009 - BT3401 0.75 -1.2 4,381,066 - BT3401 0.80 -0.0 4,381,167 - BT3401 0.89 +0.6 4,381,291 - -2.3 4,381,291 - -2.0 4,381,294 + -0.1 4,381,294 + -0.8 4,381,295 - +0.2 4,381,313 + -1.0 4,381,314 - +1.3 4,381,315 + -2.3 4,381,316 - -0.0 4,381,332 - -1.6
Or see this region's nucleotide sequence