Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2207 and BT2208 overlap by 4 nucleotides BT2208 and BT2209 are separated by 75 nucleotides BT2209 and BT2210 overlap by 17 nucleotides BT2210 and BT2211 overlap by 6 nucleotides BT2211 and BT2212 are separated by 59 nucleotides
BT2207: BT2207 - putative ATPase/GTPase (NCBI ptt file), at 2,777,429 to 2,777,842
BT2207
BT2208: BT2208 - hypothetical protein (NCBI ptt file), at 2,777,839 to 2,778,063
BT2208
BT2209: BT2209 - putative nucleotidyltransferase (NCBI ptt file), at 2,778,139 to 2,778,432
BT2209
BT2210: BT2210 - conserved hypothetical protein (NCBI ptt file), at 2,778,416 to 2,778,793
BT2210
BT2211: BT2211 - conserved hypothetical protein (NCBI ptt file), at 2,778,788 to 2,780,098
BT2211
BT2212: BT2212 - magnesium chelatase, subunit I, putative ATPase (NCBI ptt file), at 2,780,158 to 2,781,132
BT2212
Position (kb)
2778
2779
2780
2781 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2777.838 kb on + strand at 2777.858 kb on + strand at 2778.009 kb on + strand, within BT2208 at 2778.010 kb on - strand, within BT2208 at 2778.026 kb on + strand, within BT2208 at 2778.039 kb on - strand, within BT2208 at 2778.039 kb on - strand, within BT2208 at 2778.065 kb on - strand at 2778.065 kb on - strand at 2778.134 kb on + strand at 2778.223 kb on - strand, within BT2209 at 2778.340 kb on - strand, within BT2209 at 2778.424 kb on + strand at 2778.425 kb on - strand at 2778.448 kb on + strand at 2778.479 kb on - strand, within BT2210 at 2778.502 kb on + strand, within BT2210 at 2778.502 kb on + strand, within BT2210 at 2778.505 kb on - strand, within BT2210 at 2778.595 kb on + strand, within BT2210 at 2778.596 kb on - strand, within BT2210 at 2778.720 kb on + strand, within BT2210 at 2778.726 kb on + strand, within BT2210 at 2778.775 kb on + strand at 2778.786 kb on + strand at 2778.821 kb on + strand at 2778.823 kb on + strand at 2778.823 kb on + strand at 2778.839 kb on - strand at 2778.892 kb on + strand at 2778.949 kb on - strand, within BT2211 at 2779.096 kb on - strand, within BT2211 at 2779.096 kb on - strand, within BT2211 at 2779.174 kb on + strand, within BT2211 at 2779.188 kb on + strand, within BT2211 at 2779.213 kb on + strand, within BT2211 at 2779.214 kb on - strand, within BT2211 at 2779.328 kb on - strand, within BT2211 at 2779.389 kb on - strand, within BT2211 at 2779.433 kb on + strand, within BT2211 at 2779.433 kb on + strand, within BT2211 at 2779.496 kb on + strand, within BT2211 at 2779.501 kb on - strand, within BT2211 at 2779.547 kb on - strand, within BT2211 at 2779.573 kb on + strand, within BT2211 at 2779.574 kb on - strand, within BT2211 at 2779.623 kb on + strand, within BT2211 at 2779.746 kb on + strand, within BT2211 at 2779.746 kb on + strand, within BT2211 at 2779.813 kb on + strand, within BT2211 at 2779.821 kb on - strand, within BT2211 at 2779.957 kb on + strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.958 kb on - strand, within BT2211 at 2779.959 kb on + strand, within BT2211 at 2779.960 kb on - strand, within BT2211 at 2779.960 kb on - strand, within BT2211 at 2780.048 kb on + strand at 2780.076 kb on + strand at 2780.077 kb on - strand at 2780.379 kb on - strand, within BT2212 at 2780.380 kb on + strand, within BT2212 at 2780.424 kb on - strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.436 kb on + strand, within BT2212 at 2780.443 kb on + strand, within BT2212 at 2780.524 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.538 kb on + strand, within BT2212 at 2780.582 kb on + strand, within BT2212 at 2780.676 kb on + strand, within BT2212 at 2780.681 kb on + strand, within BT2212 at 2780.682 kb on - strand, within BT2212 at 2780.698 kb on - strand, within BT2212 at 2780.719 kb on + strand, within BT2212 at 2780.720 kb on - strand, within BT2212 at 2780.795 kb on - strand, within BT2212 at 2780.848 kb on - strand, within BT2212 at 2780.987 kb on - strand, within BT2212
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 2,777,838 + -3.5 2,777,858 + -0.2 2,778,009 + BT2208 0.76 -1.5 2,778,010 - BT2208 0.76 -0.0 2,778,026 + BT2208 0.83 -2.3 2,778,039 - BT2208 0.89 -2.3 2,778,039 - BT2208 0.89 -3.2 2,778,065 - -2.3 2,778,065 - -1.6 2,778,134 + -0.7 2,778,223 - BT2209 0.29 -0.0 2,778,340 - BT2209 0.68 -1.0 2,778,424 + +1.4 2,778,425 - -1.2 2,778,448 + -2.9 2,778,479 - BT2210 0.17 -1.5 2,778,502 + BT2210 0.23 +0.7 2,778,502 + BT2210 0.23 -2.0 2,778,505 - BT2210 0.24 +0.6 2,778,595 + BT2210 0.47 -1.6 2,778,596 - BT2210 0.48 +0.6 2,778,720 + BT2210 0.80 -0.7 2,778,726 + BT2210 0.82 -0.4 2,778,775 + -0.2 2,778,786 + -1.0 2,778,821 + -0.5 2,778,823 + -2.6 2,778,823 + -0.0 2,778,839 - -0.7 2,778,892 + -1.6 2,778,949 - BT2211 0.12 -1.6 2,779,096 - BT2211 0.23 -0.0 2,779,096 - BT2211 0.23 -0.1 2,779,174 + BT2211 0.29 -2.3 2,779,188 + BT2211 0.31 +0.1 2,779,213 + BT2211 0.32 -1.6 2,779,214 - BT2211 0.32 -1.6 2,779,328 - BT2211 0.41 +0.4 2,779,389 - BT2211 0.46 -0.0 2,779,433 + BT2211 0.49 +1.8 2,779,433 + BT2211 0.49 -3.0 2,779,496 + BT2211 0.54 +1.6 2,779,501 - BT2211 0.54 -3.0 2,779,547 - BT2211 0.58 -2.0 2,779,573 + BT2211 0.60 -2.8 2,779,574 - BT2211 0.60 -4.8 2,779,623 + BT2211 0.64 -1.7 2,779,746 + BT2211 0.73 +1.2 2,779,746 + BT2211 0.73 -0.0 2,779,813 + BT2211 0.78 -1.0 2,779,821 - BT2211 0.79 -0.4 2,779,957 + BT2211 0.89 -2.6 2,779,958 - BT2211 0.89 -3.2 2,779,958 - BT2211 0.89 -1.6 2,779,958 - BT2211 0.89 -0.4 2,779,959 + BT2211 0.89 -0.5 2,779,960 - BT2211 0.89 +3.0 2,779,960 - BT2211 0.89 -1.0 2,780,048 + -2.6 2,780,076 + -0.0 2,780,077 - -0.0 2,780,379 - BT2212 0.23 -3.3 2,780,380 + BT2212 0.23 -2.8 2,780,424 - BT2212 0.27 -0.0 2,780,436 + BT2212 0.29 -1.0 2,780,436 + BT2212 0.29 +0.1 2,780,443 + BT2212 0.29 -3.2 2,780,524 + BT2212 0.38 -1.0 2,780,538 + BT2212 0.39 +0.3 2,780,538 + BT2212 0.39 -0.0 2,780,582 + BT2212 0.43 -2.8 2,780,676 + BT2212 0.53 -3.2 2,780,681 + BT2212 0.54 +0.1 2,780,682 - BT2212 0.54 -1.6 2,780,698 - BT2212 0.55 +0.4 2,780,719 + BT2212 0.58 -0.0 2,780,720 - BT2212 0.58 -0.7 2,780,795 - BT2212 0.65 -1.3 2,780,848 - BT2212 0.71 -1.6 2,780,987 - BT2212 0.85 -0.2
Or see this region's nucleotide sequence