Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2211

Experiment: BHIS with Doxycycline hyclate 0.000390625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2207 and BT2208 overlap by 4 nucleotidesBT2208 and BT2209 are separated by 75 nucleotidesBT2209 and BT2210 overlap by 17 nucleotidesBT2210 and BT2211 overlap by 6 nucleotidesBT2211 and BT2212 are separated by 59 nucleotides BT2207: BT2207 - putative ATPase/GTPase (NCBI ptt file), at 2,777,429 to 2,777,842 BT2207 BT2208: BT2208 - hypothetical protein (NCBI ptt file), at 2,777,839 to 2,778,063 BT2208 BT2209: BT2209 - putative nucleotidyltransferase (NCBI ptt file), at 2,778,139 to 2,778,432 BT2209 BT2210: BT2210 - conserved hypothetical protein (NCBI ptt file), at 2,778,416 to 2,778,793 BT2210 BT2211: BT2211 - conserved hypothetical protein (NCBI ptt file), at 2,778,788 to 2,780,098 BT2211 BT2212: BT2212 - magnesium chelatase, subunit I, putative ATPase (NCBI ptt file), at 2,780,158 to 2,781,132 BT2212 Position (kb) 2778 2779 2780 2781Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 2777.838 kb on + strandat 2777.858 kb on + strandat 2778.009 kb on + strand, within BT2208at 2778.010 kb on - strand, within BT2208at 2778.026 kb on + strand, within BT2208at 2778.039 kb on - strand, within BT2208at 2778.039 kb on - strand, within BT2208at 2778.065 kb on - strandat 2778.065 kb on - strandat 2778.134 kb on + strandat 2778.223 kb on - strand, within BT2209at 2778.340 kb on - strand, within BT2209at 2778.424 kb on + strandat 2778.425 kb on - strandat 2778.448 kb on + strandat 2778.479 kb on - strand, within BT2210at 2778.502 kb on + strand, within BT2210at 2778.502 kb on + strand, within BT2210at 2778.505 kb on - strand, within BT2210at 2778.595 kb on + strand, within BT2210at 2778.596 kb on - strand, within BT2210at 2778.720 kb on + strand, within BT2210at 2778.726 kb on + strand, within BT2210at 2778.775 kb on + strandat 2778.786 kb on + strandat 2778.821 kb on + strandat 2778.823 kb on + strandat 2778.823 kb on + strandat 2778.839 kb on - strandat 2778.892 kb on + strandat 2778.949 kb on - strand, within BT2211at 2779.096 kb on - strand, within BT2211at 2779.096 kb on - strand, within BT2211at 2779.174 kb on + strand, within BT2211at 2779.188 kb on + strand, within BT2211at 2779.213 kb on + strand, within BT2211at 2779.214 kb on - strand, within BT2211at 2779.328 kb on - strand, within BT2211at 2779.389 kb on - strand, within BT2211at 2779.433 kb on + strand, within BT2211at 2779.433 kb on + strand, within BT2211at 2779.496 kb on + strand, within BT2211at 2779.501 kb on - strand, within BT2211at 2779.547 kb on - strand, within BT2211at 2779.573 kb on + strand, within BT2211at 2779.574 kb on - strand, within BT2211at 2779.623 kb on + strand, within BT2211at 2779.746 kb on + strand, within BT2211at 2779.746 kb on + strand, within BT2211at 2779.813 kb on + strand, within BT2211at 2779.821 kb on - strand, within BT2211at 2779.957 kb on + strand, within BT2211at 2779.958 kb on - strand, within BT2211at 2779.958 kb on - strand, within BT2211at 2779.958 kb on - strand, within BT2211at 2779.959 kb on + strand, within BT2211at 2779.960 kb on - strand, within BT2211at 2779.960 kb on - strand, within BT2211at 2780.048 kb on + strandat 2780.076 kb on + strandat 2780.077 kb on - strandat 2780.379 kb on - strand, within BT2212at 2780.380 kb on + strand, within BT2212at 2780.424 kb on - strand, within BT2212at 2780.436 kb on + strand, within BT2212at 2780.436 kb on + strand, within BT2212at 2780.443 kb on + strand, within BT2212at 2780.524 kb on + strand, within BT2212at 2780.538 kb on + strand, within BT2212at 2780.538 kb on + strand, within BT2212at 2780.582 kb on + strand, within BT2212at 2780.676 kb on + strand, within BT2212at 2780.681 kb on + strand, within BT2212at 2780.682 kb on - strand, within BT2212at 2780.698 kb on - strand, within BT2212at 2780.719 kb on + strand, within BT2212at 2780.720 kb on - strand, within BT2212at 2780.795 kb on - strand, within BT2212at 2780.848 kb on - strand, within BT2212at 2780.987 kb on - strand, within BT2212

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM
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2,777,838 + -3.5
2,777,858 + -0.2
2,778,009 + BT2208 0.76 -1.5
2,778,010 - BT2208 0.76 -0.0
2,778,026 + BT2208 0.83 -2.3
2,778,039 - BT2208 0.89 -2.3
2,778,039 - BT2208 0.89 -3.2
2,778,065 - -2.3
2,778,065 - -1.6
2,778,134 + -0.7
2,778,223 - BT2209 0.29 -0.0
2,778,340 - BT2209 0.68 -1.0
2,778,424 + +1.4
2,778,425 - -1.2
2,778,448 + -2.9
2,778,479 - BT2210 0.17 -1.5
2,778,502 + BT2210 0.23 +0.7
2,778,502 + BT2210 0.23 -2.0
2,778,505 - BT2210 0.24 +0.6
2,778,595 + BT2210 0.47 -1.6
2,778,596 - BT2210 0.48 +0.6
2,778,720 + BT2210 0.80 -0.7
2,778,726 + BT2210 0.82 -0.4
2,778,775 + -0.2
2,778,786 + -1.0
2,778,821 + -0.5
2,778,823 + -2.6
2,778,823 + -0.0
2,778,839 - -0.7
2,778,892 + -1.6
2,778,949 - BT2211 0.12 -1.6
2,779,096 - BT2211 0.23 -0.0
2,779,096 - BT2211 0.23 -0.1
2,779,174 + BT2211 0.29 -2.3
2,779,188 + BT2211 0.31 +0.1
2,779,213 + BT2211 0.32 -1.6
2,779,214 - BT2211 0.32 -1.6
2,779,328 - BT2211 0.41 +0.4
2,779,389 - BT2211 0.46 -0.0
2,779,433 + BT2211 0.49 +1.8
2,779,433 + BT2211 0.49 -3.0
2,779,496 + BT2211 0.54 +1.6
2,779,501 - BT2211 0.54 -3.0
2,779,547 - BT2211 0.58 -2.0
2,779,573 + BT2211 0.60 -2.8
2,779,574 - BT2211 0.60 -4.8
2,779,623 + BT2211 0.64 -1.7
2,779,746 + BT2211 0.73 +1.2
2,779,746 + BT2211 0.73 -0.0
2,779,813 + BT2211 0.78 -1.0
2,779,821 - BT2211 0.79 -0.4
2,779,957 + BT2211 0.89 -2.6
2,779,958 - BT2211 0.89 -3.2
2,779,958 - BT2211 0.89 -1.6
2,779,958 - BT2211 0.89 -0.4
2,779,959 + BT2211 0.89 -0.5
2,779,960 - BT2211 0.89 +3.0
2,779,960 - BT2211 0.89 -1.0
2,780,048 + -2.6
2,780,076 + -0.0
2,780,077 - -0.0
2,780,379 - BT2212 0.23 -3.3
2,780,380 + BT2212 0.23 -2.8
2,780,424 - BT2212 0.27 -0.0
2,780,436 + BT2212 0.29 -1.0
2,780,436 + BT2212 0.29 +0.1
2,780,443 + BT2212 0.29 -3.2
2,780,524 + BT2212 0.38 -1.0
2,780,538 + BT2212 0.39 +0.3
2,780,538 + BT2212 0.39 -0.0
2,780,582 + BT2212 0.43 -2.8
2,780,676 + BT2212 0.53 -3.2
2,780,681 + BT2212 0.54 +0.1
2,780,682 - BT2212 0.54 -1.6
2,780,698 - BT2212 0.55 +0.4
2,780,719 + BT2212 0.58 -0.0
2,780,720 - BT2212 0.58 -0.7
2,780,795 - BT2212 0.65 -1.3
2,780,848 - BT2212 0.71 -1.6
2,780,987 - BT2212 0.85 -0.2

Or see this region's nucleotide sequence