Experiment: BHIS with Doxycycline hyclate 0.000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2143 and BT2144 are separated by 85 nucleotides BT2144 and BT2145 are separated by 431 nucleotides
BT2143: BT2143 - chromosomal replication initiator protein dnaA (NCBI ptt file), at 2,697,457 to 2,698,869
BT2143
BT2144: BT2144 - putative oxidoreductase (NCBI ptt file), at 2,698,955 to 2,699,692
BT2144
BT2145: BT2145 - ribonucleoside-diphosphate reductase alpha chain (NCBI ptt file), at 2,700,124 to 2,702,514
BT2145
Position (kb)
2698
2699
2700 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2698.868 kb on - strand at 2698.868 kb on - strand at 2698.883 kb on + strand at 2698.887 kb on - strand at 2698.887 kb on - strand at 2698.887 kb on - strand at 2698.893 kb on + strand at 2698.933 kb on - strand at 2698.936 kb on + strand at 2698.937 kb on - strand at 2698.939 kb on + strand at 2698.943 kb on + strand at 2698.946 kb on + strand at 2698.947 kb on - strand at 2698.947 kb on - strand at 2698.950 kb on + strand at 2698.951 kb on - strand at 2698.956 kb on + strand at 2698.957 kb on - strand at 2699.000 kb on - strand at 2699.021 kb on - strand at 2699.028 kb on - strand at 2699.072 kb on + strand, within BT2144 at 2699.105 kb on - strand, within BT2144 at 2699.106 kb on + strand, within BT2144 at 2699.112 kb on + strand, within BT2144 at 2699.140 kb on - strand, within BT2144 at 2699.287 kb on + strand, within BT2144 at 2699.289 kb on + strand, within BT2144 at 2699.289 kb on + strand, within BT2144 at 2699.294 kb on - strand, within BT2144 at 2699.369 kb on - strand, within BT2144 at 2699.369 kb on - strand, within BT2144 at 2699.555 kb on + strand, within BT2144 at 2699.557 kb on + strand, within BT2144 at 2699.557 kb on + strand, within BT2144 at 2699.557 kb on + strand, within BT2144 at 2699.558 kb on - strand, within BT2144 at 2699.558 kb on - strand, within BT2144 at 2699.558 kb on - strand, within BT2144 at 2699.563 kb on + strand, within BT2144 at 2700.104 kb on - strand at 2700.124 kb on - strand at 2700.223 kb on + strand at 2700.268 kb on + strand at 2700.363 kb on - strand, within BT2145 at 2700.482 kb on - strand, within BT2145 at 2700.670 kb on + strand, within BT2145 at 2700.670 kb on + strand, within BT2145 at 2700.675 kb on - strand, within BT2145
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Doxycycline hyclate 0.000390625 mM remove 2,698,868 - -2.1 2,698,868 - -2.9 2,698,883 + -1.1 2,698,887 - -1.1 2,698,887 - -4.9 2,698,887 - -3.4 2,698,893 + -1.1 2,698,933 - -5.5 2,698,936 + -4.1 2,698,937 - -2.4 2,698,939 + -2.8 2,698,943 + -1.1 2,698,946 + -0.4 2,698,947 - -3.6 2,698,947 - -2.6 2,698,950 + -2.9 2,698,951 - -4.1 2,698,956 + -2.6 2,698,957 - -2.6 2,699,000 - -2.4 2,699,021 - -1.1 2,699,028 - -5.7 2,699,072 + BT2144 0.16 -2.3 2,699,105 - BT2144 0.20 -3.4 2,699,106 + BT2144 0.20 -0.3 2,699,112 + BT2144 0.21 -1.7 2,699,140 - BT2144 0.25 -0.1 2,699,287 + BT2144 0.45 -1.7 2,699,289 + BT2144 0.45 -2.3 2,699,289 + BT2144 0.45 -1.6 2,699,294 - BT2144 0.46 -0.1 2,699,369 - BT2144 0.56 -1.1 2,699,369 - BT2144 0.56 -3.2 2,699,555 + BT2144 0.81 -1.1 2,699,557 + BT2144 0.82 -2.1 2,699,557 + BT2144 0.82 -2.9 2,699,557 + BT2144 0.82 -1.8 2,699,558 - BT2144 0.82 -3.8 2,699,558 - BT2144 0.82 -2.4 2,699,558 - BT2144 0.82 -3.2 2,699,563 + BT2144 0.82 -3.1 2,700,104 - -2.9 2,700,124 - -0.5 2,700,223 + -1.6 2,700,268 + -0.7 2,700,363 - BT2145 0.10 -1.0 2,700,482 - BT2145 0.15 -2.4 2,700,670 + BT2145 0.23 -2.0 2,700,670 + BT2145 0.23 -2.0 2,700,675 - BT2145 0.23 -1.6
Or see this region's nucleotide sequence